Spectrophotometric determination of amoxicillin by reaction with N,N-dimethyl-p-phenylenediamine and potassium hexacyanoferrate(III)

Al-Abachi, Mouayed Q.; Haddi, Hind; Al-Abachi, Anas M.

doi:10.1016/j.aca.2005.08.030

Cited by 78 publications

(33 citation statements)

References 10 publications

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“…Spectrophotometric determination of AMO at 660 nm was achieved by reaction in batch or FIA with N,Ndimethyl-p-phenylenediamine in the presence of potassium hexacyanoferrate (III) in alkaline medium [127]. [128].…”

Section: Nonchromatographic Techniquesmentioning

confidence: 99%

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

Samanidou

Evaggelopoulou

Papadoyannis³

2006

J of Separation Science

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Natural penicillin (benzylpenicillin) is the oldest antibiotic observed by Alexander Fleming in 1928. To broaden its spectrum of activity, natural penicillin was modified, giving rise to a group of antibiotics under the name 'penicillins'. Although an increasing number of bacteria appear to be resistant to them, penicillins are used to treat a variety of bacterial infections including Gram-positive, Gram-negative aerobic and anaerobic bacteria. Consequently, they are widely used in human and veterinary medicine to prevent and treat diseases. This review covers the analytical methodologies, mainly chromatographic, employed to the penicillins determination in pharmaceutical formulations, biological fluids and in production-scale fermentations reported in the literature. Results of published assays are comparatively presented focusing on sample preparation regarding isolation and purification, chromatographic conditions and method validation. Information on chemical structure, spectrum of activity and action mechanism of common penicillins has also been given.

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Section: Nonchromatographic Techniquesmentioning

confidence: 99%

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

Samanidou

Evaggelopoulou

Papadoyannis³

2006

J of Separation Science

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“…Side effects are similar to those for other betalactam antibiotics, including nausea, vomiting, rashes, and antibiotic-associated colitis [2,[11][12]. To date various analytical methods have been developed for the determination of AMX including high-performance liquid chromatography (HPLC) [13][14][15], UV-Vis spectroscopy [16][17][18], liquid chromatography coupled to mass spectrometry [19], capillary electrophoresis with mass spectrometry [20], and electrochemical methods [2,14,[21][22][23][24] are also reported for AMX detection. Among all these methods, chromatographic and capillary electrophoresis methods are most commonly used to exploit various sensors for determination of AMX.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of highly-ordered Zinc Oxide nanorods on gold/glass electrode, and its application as a voltammetric sensor

Hatamie

Echresh

Zargar

et al. 2015

Electrochimica Acta

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“…Other techniques for AMO analysis in pharmaceuticals involve CE [10] and FIA by reaction with N,N-dimethyl-pphenylenediamine and potassium hexacyanoferrate (III) where the blue color product was measured at 660 nm [14].…”

Section: Introductionmentioning

confidence: 99%

Development of a validated HPLC method for the determination of four penicillin antibiotics in pharmaceuticals and human biological fluids

Samanidou¹,

Evaggelopoulou

Papadoyannis

2006

J. Sep. Sci.

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A quantitative method for the determination of four penicillin antibiotics, amoxicillin (AMO), oxacillin (OXA), cloxacillin (CLO), and dicloxacillin (DICLO), has been developed. Separation was achieved on an Inertsil ODS-3 (250 x 4 mm, 5 microm) column after selective extraction of penicillin drugs from biological matrices by means of SPE. Gradient elution with a mobile phase consisting of 0.1% TFA (pH 1) and ACN, and PDA detection with monitoring at 240 nm was applied. Salicylic acid (5 ng/microL) was used as the internal standard. RP-8 Adsorbex Merck cartridges provided high absolute recoveries (98-101%). The developed method was fully validated in terms of selectivity, linearity, accuracy, precision, stability, and sensitivity. Repeatability (n = 8) and between-day precision (n = 8) revealed RSD <10%. Recoveries from biological samples ranged from 91 to 103%. The detection limits were estimated as 3.3 ng for AMO, OXA, and CLO, and 6.6 for DICLO in blood plasma. LOD in whole blood and urine was 6.6 ng. Injection volume was 20 microL. The method was applied to commercially available AMO containing pharmaceuticals and spiked biological matrices. The method was also applied to biological samples after AMO oral administration, where the drug was successfully identified and quantified.

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Spectrophotometric determination of amoxicillin by reaction with N,N-dimethyl-p-phenylenediamine and potassium hexacyanoferrate(III)

Cited by 78 publications

References 10 publications

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

Fabrication and characterization of highly-ordered Zinc Oxide nanorods on gold/glass electrode, and its application as a voltammetric sensor

Development of a validated HPLC method for the determination of four penicillin antibiotics in pharmaceuticals and human biological fluids

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