Spectrophotometric and spectrofluorimetric methods for analysis of acyclovir and acebutolol hydrochloride

Abdellatef, Hisham E.; Elhenawee, Magda; El‐Sayed, Heba M.; Ayad, Magda M.

doi:10.1016/j.saa.2006.01.001

Cited by 22 publications

(12 citation statements)

References 15 publications

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“…CAS because of its high oxidation potential and excellent solution stability has been widely used as an effective analytical reagent in spectrophotometric methods for the determination of many pharmaceutical compounds [27][28][29][30][31]. The analytical reactions involved two steps; the first one was concerned with oxidation of TOL with a known excess of CAS in acidic medium at room temperature (25°C±2°C).…”

Section: Resultsmentioning

confidence: 99%

Sensitive Spectrophotometric Assay of Muscarinic Receptor Antagonist Tolterodine Tartrate in Bulk Drug and Pharmaceutical Formulations

El-Sheikh

Hаssаn

Gouda

et al. 2017

Asian J Pharm Clin Res

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Objective: Simple, sensitive, and accurate spectrophotometric methods have been developed for the assay of tolterodine tartrate (TOL) in bulk drug and pharmaceutical formulations. Methods:The proposed methods are based on oxidation reaction of TOL with a known excess of cerium(IV) ammonium sulfate as an oxidizing agent in acid medium followed by determination of unreacted oxidant by adding a fixed amount of dye, e.g., amaranth (AM), rhodamine 6G (Rh6G), and indigo carmine (IC) followed by measuring the absorbance at 520, 530, and 610 nm, respectively. The effect of experimental conditions was studied and optimized.Results: The Beer's law was obeyed in the concentration ranges of 1.0-10, 1.0-12, and 0.5-9.0 μg/mL using AM, Rh6G, and IC dyes, respectively, with a correlation coefficient ≥0.9995. The calculated molar absorptivity values are 1.868×10 4 , 1.008×10 4 , and 1.623×104 L/mol/cm using AM, Rh6G, and IC dyes, respectively. The limits of detection and quantification were reported. Intraday and interday accuracy and precision of the methods have been evaluated. No interference was observed from the additives. Conclusion:The proposed methods were successfully applied to the assay of TOL in tablets preparations, and the results were statistically compared with those of the reported method by applying Student's t-test and F-test. The reliability of the methods was further ascertained by performing recovery studies using the standard addition method.

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Section: Resultsmentioning

confidence: 99%

Sensitive Spectrophotometric Assay of Muscarinic Receptor Antagonist Tolterodine Tartrate in Bulk Drug and Pharmaceutical Formulations

El-Sheikh

Hаssаn

Gouda

et al. 2017

Asian J Pharm Clin Res

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“…Ce IV has been used as an oxidizing agent in the determination of many pharmaceutical compounds by spectrophotometric 23,24 and spectrofluorimetric 25-27 methods or by both of them 28,29 and chemiluminescence. 30 The proposed method involves the addition of Ce IV to cephalexin in acidic medium, followed by the determination of residual Ce IV by reacting with a fixed amount of methyl orange, and the absorbance was measured at 510 nm ( Figure 2).…”

Section: Resultsmentioning

confidence: 99%

Development and Validation of a New Spectrophotometric Method for the Determination of Cephalexin Monohydrate in Pure Form and Pharmaceutical Formulations

Khan¹,

Jalal²,

Jan³

et al. 2015

Journal of the Brazilian Chemical Society

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A simple, fast, sensitive and accurate spectrophotometric method has been developed for the quantitative determination of cephalexin monohydrate in dosage form and in commercial formulations. The method involves the addition of Ce IV to cephalexin in acidic medium, followed by the determination of residual Ce IV by reacting with a fixed amount of methyl orange, and the absorbance was measured at 510 nm. Beer's law was obeyed in the concentration range of 0.6-20 µg mL -1 with correlation coefficient of 0.992. The molar absorptivity was calculated and was found to be 9.8 × 10 4 L mol -1 cm -1. Different variables affecting the reaction conditions such as concentration and volume of Ce IV , type and concentration of acids used, reaction time and temperature were carefully studied and optimized. The limit of detection (LOD) and limit of quantification (LOQ) were calculated and found to be 0.221 and 0.736 µg mL -1 , respectively. The proposed method was found to have good reproducibility with a relative standard deviation of 4.4% (n = 9). The interference effects of common excipients found in pharmaceutical preparations were studied. The developed method was validated statistically by performing recoveries studies and successfully applied for the determination of cephalexin in bulk powder and pharmaceutical preparations. Percent recoveries were calculated and found to range from 90.6 to 102.9% for bulk powder and from 98.7 to 104.8% for pharmaceutical preparations.

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“…Oxidation-reduction reactions have been used as the basis for the development of simple and sensitive spectrophotometric methods for the determination of many pharmaceutical compounds [95][96][97][98][99] . Cerric (IV) ammonium sulphate, because of its high oxidation potential and excellent solution stability, has been widely used as an effective analytical reagent in these methods 95 .…”

Section: Resultsmentioning

confidence: 99%

Cerimetric Determination of Four Antihypertensive Drugs in Pharmaceutical Preparations

El‐Didamony

Erfan

2011

J. Chil. Chem. Soc.

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A sensitive spectrophotometric method is described for the determination of atenolol (ATE), timolol maleate (TIM), captopril (CPL) and diltiazem hydrochloride (DIL.HCl) in bulk drugs and in pharmaceutical preparations. The method is based on the oxidation of the studied drugs by a known excess of cerric (IV) in acid medium followed by determination of unreacted oxidant by adding a fixed amount of methyl orange dye (MO) and the increasing in absorbance is measured at 510 nm. In this method the amount of cerium (IV) reacted corresponds to drugs concentration. The experimental conditions were optimized. Regression analysis of a Beer's plot showed good correlation in the concentration ranges of 3.2-6.4, 8.0-18, 3.4-5.2 and 3.6-5.2 µg/ml for ATE, TIM, CPL and DIL.HCl, respectively. The calculated molar absorptivity values are 5.28×10 4 , 3.27×10 4 , 6.43×10 4 and 1.12×10 5 L /mol cm, respectively and the corresponding Sandell's sensitivity values are 5.043, 13.2, 3.381 and 4.024 ng/cm 2 , respectively. The limit of detection (LOD) and quantification (LOQ) are reported. No interference was observed from the additives and the applicability of the method was tested by analyzing the pharmaceutical preparations containing the investigated drugs. Statistical comparison of the results with those of official methods shows excellent agreement and indicates no significant difference in precision.

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Spectrophotometric and spectrofluorimetric methods for analysis of acyclovir and acebutolol hydrochloride

Cited by 22 publications

References 15 publications

Sensitive Spectrophotometric Assay of Muscarinic Receptor Antagonist Tolterodine Tartrate in Bulk Drug and Pharmaceutical Formulations

Sensitive Spectrophotometric Assay of Muscarinic Receptor Antagonist Tolterodine Tartrate in Bulk Drug and Pharmaceutical Formulations

Development and Validation of a New Spectrophotometric Method for the Determination of Cephalexin Monohydrate in Pure Form and Pharmaceutical Formulations

Cerimetric Determination of Four Antihypertensive Drugs in Pharmaceutical Preparations

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