Development and Validation of a New Spectrophotometric Method for the Determination of Cephalexin Monohydrate in Pure Form and Pharmaceutical Formulations

Abstract

A simple, fast, sensitive and accurate spectrophotometric method has been developed for the quantitative determination of cephalexin monohydrate in dosage form and in commercial formulations. The method involves the addition of Ce IV to cephalexin in acidic medium, followed by the determination of residual Ce IV by reacting with a fixed amount of methyl orange, and the absorbance was measured at 510 nm. Beer's law was obeyed in the concentration range of 0.6-20 µg mL -1 with correlation coefficient of 0.992. The molar absorptivity was calculated and was found to be 9.8 × 10 4 L mol -1 cm -1. Different variables affecting the reaction conditions such as concentration and volume of Ce IV , type and concentration of acids used, reaction time and temperature were carefully studied and optimized. The limit of detection (LOD) and limit of quantification (LOQ) were calculated and found to be 0.221 and 0.736 µg mL -1 , respectively. The proposed method was found to have good reproducibility with a relative standard deviation of 4.4% (n = 9). The interference effects of common excipients found in pharmaceutical preparations were studied. The developed method was validated statistically by performing recoveries studies and successfully applied for the determination of cephalexin in bulk powder and pharmaceutical preparations. Percent recoveries were calculated and found to range from 90.6 to 102.9% for bulk powder and from 98.7 to 104.8% for pharmaceutical preparations.

Exploiting of Green Synthesized Metal Oxide Nanoparticles for Spectrophotometric Determination of Levofloxacin, Cephalexin, and Cefotaxime Sodium in Commercial Products

“…Whereas, the percentage recoveries were found to be 99.48 ± 0.4%, 99.67 ± 0.6%, and 99.18 ± 0.8% for the three drugs in the presence of MnO 2 NPs, respectively. All results were statistically assessed using Student’s t -test and variance ratio F-test [ 68 ] and compared with those obtained from previously reported methods [ 69 , 70 , 71 ]. It was observed that the high sensitivity of the proposed spectrophotometric method for the determination of LVX, CPX, and CTX in the presence of NiONPs and MnO 2 NPs can be attributed to the catalytic properties of these nanoparticles.…”

Condensation methodology for quantification of Polymyxin B fluorimetrically: application to pharmaceutical formulations and greenness assessment

“…Belongs to published papers, more than one chromatographic approach as HPLC [2][3][4][5][6][7] and LC-Mass [8][9][10][11][12][13][14][15][16][17][18][19] also microbiological articles were reported [20,21]. On the other hand, spectroscopic techniques were listed, including spectrophotometric [22][23][24][25] and spectrofluorimetric [26][27][28] approaches. Owing to its simplicity, sensitivity, and no need for a complex apparatus or sample preparation, the spectrofluorimetry technique is frequently employed in recent drug analyses.…”

Spectrophotometric and Multivariate Calibration Techniques for Simultaneous Determination of Different Drugs in Pharmaceutical Formulations and Human Urine: Evaluation of Greenness Profile

“…AT undergoes little or no metabolism in the liver and is mainly excreted in urine (over 85% of the absorbed drug is excreted in urine as unchanged drug) [1]. Different analytical techniques have been reported for the analysis of AT such as high performance liquid chromatography (HPLC) [2][3][4], spectrophotometric [2,5,6], capillary electrophoresis [7,8], gas chromatography (GC) [9], and spectrofluorimetric [10] methods. Paracetamol (PR) or acetaminophen (Figure 1(b)) is an over-the-counter (OTC) analgesic and antipyretic drug.…”