Spectrophotometric analyses of ribose and 2-deoxy-d-ribose alone and in mixtures

Garrett, Edward R.; Blanch, Jaime.; Seydel, Joachim K.

doi:10.1002/jps.2600561206

Cited by 7 publications

(5 citation statements)

References 14 publications

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“…1) is well demonstrated. The klcomDt, value for ribose was the same as that previously obtained experimentally (5).…”

Section: % 3)supporting

confidence: 72%

“…The UV spectrophotometric absorbance of furfural (5) or of the colored compounds formed on its reaction with other reagents (6) have served as bases for analysis of these aldopentoses as well as compounds that contain them.…”

mentioning

confidence: 99%

“…A sensitive analytical technique for the determination of ribose has recently been developed in these laboratories (5). This method depends on the U V absorbance of furfural produced from degrading solutions of ribose under well-defined conditions of acidity, temperature, and time.…”

mentioning

confidence: 99%

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Kinetics and Mechanisms of the Acid Degradation of the Aldopentoses to Furfural

Garrett

Dvorchik

1969

Journal of Pharmaceutical Sciences

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“…1) is well demonstrated. The klcomDt, value for ribose was the same as that previously obtained experimentally (5).…”

Section: % 3)supporting

confidence: 72%

mentioning

confidence: 99%

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Kinetics and Mechanisms of the Acid Degradation of the Aldopentoses to Furfural

Garrett

Dvorchik

1969

Journal of Pharmaceutical Sciences

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“…The relative levels of 2DDR in culture medium and mouse serum were measured as described previously (33). Briefly, the samples were degraded in 1.0 M HCl at 80°C, and the absorbance at 261 nm and at 277 nm was taken.…”

Section: Methodsmentioning

confidence: 99%

Thymidine phosphorylase exerts complex effects on bone resorption and formation in myeloma

Liu

et al. 2016

Sci. Transl. Med.

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Myelomatous bone disease is characterized by the development of lytic bone lesions and a concomitant reduction in bone formation, leading to chronic bone pain and fractures. To understand the underlying mechanism, we investigated the contribution of myeloma-expressed thymidine phosphorylase (TP) to bone lesions. In osteoblast progenitors, TP upregulated the methylation of RUNX2 and osterix, leading to decreased bone formation. In osteoclast progenitors, TP upregulated the methylation of IRF8, thereby enhanced expression of NFATc1, leading to increased bone resorption. TP reversibly catalyzes thymidine into thymine and 2DDR. Myeloma-secreted 2DDR bound to integrin αVβ3/α5β1 in the progenitors, activated PI3K/Akt signaling, and increased DNMT3A expression, resulting in hypermethylation of RUNX2, osterix, and IRF8. This study elucidates an important mechanism for myeloma-induced bone lesions, suggesting that targeting TP may be a viable approach to healing resorbed bone in patients. As TP overexpression is common in bone-metastatic tumors, our findings could have additional mechanistic implications.

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“…Many applications of ultraviolet spectrophotometry to biological analysis continue to be reported. Among these are a method for elution and ultraviolet spectrophotometric measurement of phenylthiohydantoin derivatives of amino acids (355), determination of protein concentration without interference from nucleic acid by using wavelengths between 224 and 240 mg (160), determination of peptides after reaction with glyoxal or methylglyoxal (307), determination of peroxidized polyenoic fatty acids in rat lung lipids after exposure to trace levels of nitrogen dioxide by measurement at 230 to 235 mg (369), measurement of the rate of return of monoamine oxidase activity after inhibition by adding a benzylamine hydrochloride and measuring the appearance of the corresponding benzaldehyde (112), assay of leucine aminotransferase by measuring the 2,4-dinitrophenylhydrazones of the keto acids produced by transamination (322), and the determination of ribose and 2-deoxy-nribose by measuring the chromophore produced by heating with acid (140).…”

Section: Biological and Pharmaceutical Analysismentioning

confidence: 99%