Spectrofluorimetric determination of thioridazine and flupentixol in dosage forms; application to content uniformity test

Abstract

A reliable, sensitive, cheap and non-extractive spectrofluorimetric method has been developed and validated for determination of thioridazine and flupentixol based on ternary complex formation with eosin and lead(II) in the presence of methylcellulose as surfactant at pH 3.2. Under the optimum conditions, the quantitative quenching effect of investigated drugs on the native fluorescence of eosin has been investigated. The quenching of the eosin fluorescence was measured at 517 nm after excitation at 462 nm. The different experimental parameters affecting the development and stability of the reaction products were carefully studied and optimized, and the results were satisfactory. The calibration plots were constructed over the range of 0.5-3.0 µg mL(-1) . The developed method was successfully applied for determination of investigated drugs in commercial tablets without interference from common excipients. It was further applied for content uniformity testing of flupentixol in its tablets. Statistical comparisons of the results with those of the reference methods revealed excellent agreement and indicated no significant difference in accuracy and precision. Copyright © 2015 John Wiley & Sons, Ltd.

Application of a xanthene dye, eosin Y, as spectroscopic probe in chemical and pharmaceutical analysis; a review

“…These chelates can induce the fluorescence quenching of eosin Y upon the formation of ternary complex (Figure 4). Table 5 summarizes the use of ternary complex formation for determination of some basic compound (Omar, 2010;Abdellatef, 2007;el Walily, Belal & Bakry, 1996;Al-Tamimi, 2013;Kelani, Bebawy & Abdel-Fattah, 1999;Attia & Omar, 2016;Wu et al, 2017;Shi et al, 2016) through fluorescence quenching of eosin Y. Stern-Volmer equation was applied in several studies (Liu et al, 2008;Gan et al, 2012;Kaur & Malik, 2013;Wahba, El-Enany & Belal, 2015;Azmi et al, 2017) to investigate the mechanism of the fluorescence quenching due to the complex formation. All the mentioned reports agreed that the quenching process followed static mechanism as the quenching constants were greater than the highest reported value for dynamic quenching.…”

Utilization of Eosin Dye as an Ion Pairing Agent for Determination of Pharmaceuticals: A Brief Review

“…Moreover, the high sensitivity and specificity offered by this method are also higher and the drug compounds can be analyzed up to nano levels. As data are shown in table 1, these methods were widely used in quantitation of many pharmaceuticals compounds using eosin Y due to its simplicity, low cost, high sensitivity and wide concentration range [52][53][54][55][56][57][58][59][60][61][62][63][64][65][66][67][68].…”

Benzofurazan‐based fluorophore for the selective determination of flupentixol dihydrochloride: Application to content uniformity testing

“…According to the literature review, there are few analytical techniques published in different journals for the estimation of flupentixol in bulk form, biological fluids, and tablet form. These methods include a few non‐sensitive spectrophotometric, non‐selective and complicated spectrofluorimetric, different chromatographic and potentiometric methods. It was seen that the revealed spectrophotometric strategies for the assurance of the drug in tablets have high limit of quantitation (low sensitivity).…”

“…It was seen that the revealed spectrophotometric strategies for the assurance of the drug in tablets have high limit of quantitation (low sensitivity). However, the spectrofluorimetric methods applied to tablets suffer from the lack of selectively. The first spectrofluorimetric method is based on measuring the fluorescence intensity which resulted from the reaction of the drug with orthophosphoric acid.…”

“…The three previous methods must be conducted under strict reaction conditions in order to bypass the side reactions and different reaction products. The last spectrofluorimetric method depended on the development of a ternary complex between the drug, eosin, and metal (lead) in the presence of methylcellulose as a surfactant. The exact mechanism of the ternary complex is unknown and not ascertained by the authors.…”