Specificity of hemoglobin A1c measurement by cation exchange liquid chromatography. Evaluation of a Mono S column method

Koskinen, Leena

doi:10.1016/0009-8981(96)06349-8

Cited by 23 publications

(14 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Swedish DCM generates the lowest DCM HbA 1c values. The Mono S system, developed in 1983, shows an almost homogeneous HbA 1c peak in the chromatogram, but it contains carbamylated Hb as well as free ␣-globulin chains (25,31,32 ), so that the values are higher than those measured with the IFCC Reference Method. In contrast to the DCM methods, new dedicated HPLC systems are today eliminating many of these interfering adducts such as carbamylated Hb by use of more modern chromatographic material and improved gradients (33 ).…”

Section: Discussionmentioning

confidence: 99%

IFCC Reference System for Measurement of Hemoglobin A1c in Human Blood and the National Standardization Schemes in the United States, Japan, and Sweden: A Method-Comparison Study

et al. 2004

View full text Add to dashboard Cite

Background: The national programs for the harmonization of hemoglobin (Hb)A1c measurements in the US [National Glycohemoglobin Standardization Program (NGSP)], Japan [Japanese Diabetes Society (JDS)/Japanese Society of Clinical Chemistry (JSCC)], and Sweden are based on different designated comparison methods (DCMs). The future basis for international standardization will be the reference system developed by the IFCC Working Group on HbA1c Standardization. The aim of the present study was to determine the relationships between the IFCC Reference Method (RM) and the DCMs. Methods: Four method-comparison studies were performed in 2001–2003. In each study five to eight pooled blood samples were measured by 11 reference laboratories of the IFCC Network of Reference Laboratories, 9 Secondary Reference Laboratories of the NGSP, 3 reference laboratories of the JDS/JSCC program, and a Swedish reference laboratory. Regression equations were determined for the relationship between the IFCC RM and each of the DCMs. Results: Significant differences were observed between the HbA1c results of the IFCC RM and those of the DCMs. Significant differences were also demonstrated between the three DCMs. However, in all cases the relationship of the DCMs with the RM were linear. There were no statistically significant differences between the regression equations calculated for each of the four studies; therefore, the results could be combined. The relationship is described by the following regression equations: NGSP-HbA1c = 0.915(IFCC-HbA1c) + 2.15% (r2 = 0.998); JDS/JSCC-HbA1c = 0.927(IFCC-HbA1c) + 1.73% (r2 = 0.997); Swedish-HbA1c = 0.989(IFCC-HbA1c) + 0.88% (r2 = 0.996). Conclusion: There is a firm and reproducible link between the IFCC RM and DCM HbA1c values.

show abstract

Section: Discussionmentioning

confidence: 99%

IFCC Reference System for Measurement of Hemoglobin A1c in Human Blood and the National Standardization Schemes in the United States, Japan, and Sweden: A Method-Comparison Study

et al. 2004

View full text Add to dashboard Cite

show abstract

“…HbA1c was measured using the Mono-S method [12]. For conversion to measurements with the DCCT/NGSP method, the following conversion formula may be used: HbA1c (NGSP) (%) = 0.956 × HbA1c (Mono S) (%) + 1.182 (see e.g., http://www.hba1c.nu/).…”

Section: Methodsmentioning

confidence: 99%

Annual Medical Costs of Swedish Patients with Type 2 Diabetes Before and After Insulin Initiation

Bexelius¹,

Lundberg

Wang³

et al. 2013

Diabetes Ther

View full text Add to dashboard Cite

IntroductionAlthough insulin is one of the most effective interventions for the treatment of type 2 diabetes, its disadvantages incur substantial medical cost. This study was designed to evaluate the medical costs of Swedish type 2 diabetic patients initiating insulin on top of metformin and/or sulfonylurea (SU), and to evaluate if costs before and after insulin initiation differ for patients where insulin is initiated above or below the recommended glycosylated hemoglobin (HbA1c) level (7.5%).MethodsThis was a register-based retrospective cohort study in which patients were identified from the Sörmland county council diabetes register. Patients being prescribed at least one prescription of metformin and/or SU from 2003 to 2010, and later prescribed insulin, were included.ResultsOne hundred patients fulfilled the inclusion criteria and had at least 1 year of follow-up. The mean age was 61 years and 59% of patients were male. Mean time since diagnosis was 4.1 years, and since initiation of insulin was 2.2 years. The mean HbA1c level at index date was 8.0%. Total mean costs for the whole cohort were SEK 17,230 [standard deviation (SD) 17,228] the year before insulin initiation, and SEK 31,656 (SD 24,331) the year after insulin initiation (p < 0.0001). When stratifying by HbA1c level, patients with HbA1c <7.5% had total healthcare costs of SEK 17,678 (SD 12,946) the year before the index date and SEK 35,747 (SD 30,411) the year after (p < 0.0001). Patients with HbA1c levels ≥7.5% had total healthcare costs of SEK 16,918 (SD 19,769) the year before the index date and SEK 28,813 (SD 18,779) the year after (p < 0.0001).ConclusionDespite the small sample size, this study demonstrates that mean annual medical costs almost double the year after patients are initiated on insulin. The costs increased the year after insulin initiation, regardless of the HbA1c level at initiation of insulin, and the largest increase in costs were due to increased filled prescriptions.

show abstract

“…HbA 1C was isolated and purified to homogeneity by cation-exchange chromatography on a column with Bio-Rex 70 pre-equilibrated with 50 mM potassium-phosphate buffer, pH 6.6 [26]. Before purification the labile glycohemoglobin fraction (pre-HbA 1C ), presented by Schiff base, was eliminated by addition of 20 volumes of sodium-acetate buffer, pH 5.5, with 0.1 mM NaCl to HbA 1C and incubation for 45 min at 37°C [27]. After that hemoglobin was dialyzed against 300 volumes of 50 mM potassium-phosphate buffer, pH 6.6, for 36 h with double buffer changing.…”

Section: Methodsmentioning

confidence: 99%

1,8-Anilinonaphthalene sulfonate binds to central cavity of human hemoglobin

Syakhovich

Parul

Ruta

et al. 2004

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

Specificity of hemoglobin A1c measurement by cation exchange liquid chromatography. Evaluation of a Mono S column method

Cited by 23 publications

References 24 publications

IFCC Reference System for Measurement of Hemoglobin A1c in Human Blood and the National Standardization Schemes in the United States, Japan, and Sweden: A Method-Comparison Study

IFCC Reference System for Measurement of Hemoglobin A1c in Human Blood and the National Standardization Schemes in the United States, Japan, and Sweden: A Method-Comparison Study

Annual Medical Costs of Swedish Patients with Type 2 Diabetes Before and After Insulin Initiation

1,8-Anilinonaphthalene sulfonate binds to central cavity of human hemoglobin

Contact Info

Product

Resources

About