1,8-Anilinonaphthalene sulfonate binds to central cavity of human hemoglobin

Syakhovich, V.; Parul, D.A; Ruta, Ekaterina Ya.; Bushuk, B. A.; Bokut, S.B.

doi:10.1016/j.bbrc.2004.03.111

Cited by 20 publications

(12 citation statements)

References 29 publications

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“…Glutaraldehyde is often used as a crosslinking agent in the aldehyde-type adhesive and is known to cause serious side effects [12]. Formaldehyde is a degradation product of the cyanoacrylate-type tissue adhesive and its cytotoxicity is reported [13]. Although cytotoxicity of formaldehyde could not be evaluated in this experiment due to its volatile, cytotoxicity of glutaraldehyde might provide an indication of that of formaldehyde.…”

Section: Cytotoxicity Of the Amphiphilic Mpc Polymersmentioning

confidence: 96%

“…To evaluate the polarity of the hydrophobic domains formed by the polymer aggregates, the fluorescence spectra of ANS in the amphiphilic MPC polymers were analyzed. When an ANS molecule exists under hydrophobic condition, an increase in the fluorescence intensity and a blue shift of the luminescence maximum are observed [13]. A PlC hydrogel can be prepared from 5 wt<J/o aqueous solutions of the cationic and anionic polymers, but an aggregate or a microgel can be formed at lower concentrations.…”

Section: Adhesive Strength Measurement To Tissue In Vitromentioning

confidence: 99%

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Bioadhesion of Polyion Complex (PlC) Hydrogels Composed of Amphiphilic Phospholipid Polymers

Kimura¹,

Takai²,

Ishihara³

2007

Trans. Mat. Res. Soc. Japan

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To investigate the tissue adhesive function of a hydrogel composed ofbiocompatible amphiphilic polymers, random and graft polymers were prepared from 2-methacryloyloxyethyl phosphorylcholine (MPC), electrolyte monomers and hydrophobic n-butyl methacrylate (BMA). The results of the fluorescence study suggested enhanced electrostatic interaction in the graft polymers compared to the random polymers. This is due to the strategically designed architectures and the hydrophobic BMA units. The results of the cytotoxicity test showed that the cytotoxicity of the MPC polymers was lower than that of glutaraldehyde, which is a crosslinking agent in the aldehyde-type tissue adhesives and is known to cause serious side effects. The cationic MPC polymers demonstrated higher cytotoxicity compared to the anionic ones, which demonstrated no significant cytotoxicity. The tissue adhesion of the PlC hydrogels was lower than that of a commercially available fibrin glue. However, the tissue adhesive strength increased with an increase in the polymer concentration and could be controlled by the water content of the hydrogel. In addition, balance of the electrolyte units for the PIC formation and the tissue adhesion is crucial for expression of adhesion. Although further investigation of the biocompatibility is required, it can be concluded that the PlC hydrogels fonned by the amphiphilic MPC polymers can be a promising tissue adhesive which demonstrate tissue adhesion and biocompatibility according to the architectures and chemical structures.

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Section: Cytotoxicity Of the Amphiphilic Mpc Polymersmentioning

confidence: 96%

Section: Adhesive Strength Measurement To Tissue In Vitromentioning

confidence: 99%

Bioadhesion of Polyion Complex (PlC) Hydrogels Composed of Amphiphilic Phospholipid Polymers

Kimura¹,

Takai²,

Ishihara³

2007

Trans. Mat. Res. Soc. Japan

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“…In addition to the importance of the development and standardization of HbA 1C quantification methods used in clinical assays, it should be noted that this minor haemoglobin form is an interesting object in itself. For example, it has been shown that the carbohydrate modification of haemoglobin is the reason for the steric barrier increase to the interaction of allosteric effectors with HbA 1C (DeRosa et al, 1998;Syakhovich et al, 2004) and affects its transport function through redistribution of long-lived and short-lived components in photo-dissociated oxygen recombination kinetics (Okenchi et al, 2001;Bokut et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Crystallization and preliminary crystallographic analysis of human glycosylated haemoglobin

Syakhovich¹,

Saraswathi²,

Ruff³

et al. 2006

Acta Cryst Sect F

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Human glycosylated haemoglobin A1C is a stable minor variant formed in vivo by post-translational modification of the main form of haemoglobin by glucose. Crystals of oxyHbA1C were obtained using the hanging-drop vapour-diffusion method and PEG as precipitant. The diffraction pattern of the crystal extends to a resolution of 2.3 A at 120 K. The crystals belong to space group C2, with unit-cell parameters a = 237.98, b = 59.27, c = 137.02 A, alpha = 90.00, beta = 125.40, gamma = 90.00 degrees. The presence of two and a half molecules per asymmetric unit gives a crystal volume per protein weight (VM) of 9.70 A3 Da(-1) and a solvent content of 49%.

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“…In particular, three lifetimes were also reported for 1,8-ANS association with oxyhemoglobin with values of 0.85 ns, 3.78 ns, and 11.74 ns (Syakhovich et al, 2004). In ITC, the binding of the dye to the protein is determined by measuring the heat released upon ligand association to protein, which is independent of the fluorescence properties of the dye or the dye-protein complexes (Pierce et al, 1999).…”

Section: Time-resolved Fluorescence Spectroscopymentioning

confidence: 99%

“…In the presence of Cygb, two additional lifetimes were determined with the time constants of 2.4 ± 0.1 ns and 7.7 ± 0. (Syakhovich et al, 2004;Uversky et al, 1996).…”

Section: Time-resolved Fluorescence Spectroscopymentioning

confidence: 99%

Conformational Dynamics Associated with Ligand Binding to Vertebrate Hexa-coordinate Hemoglobins

Astudillo¹

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1,8-Anilinonaphthalene sulfonate binds to central cavity of human hemoglobin

Cited by 20 publications

References 29 publications

Bioadhesion of Polyion Complex (PlC) Hydrogels Composed of Amphiphilic Phospholipid Polymers

Bioadhesion of Polyion Complex (PlC) Hydrogels Composed of Amphiphilic Phospholipid Polymers

Crystallization and preliminary crystallographic analysis of human glycosylated haemoglobin

Conformational Dynamics Associated with Ligand Binding to Vertebrate Hexa-coordinate Hemoglobins

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