Specificities of oxidative stress in the blood and synovial fluid in knee osteoarthritis

Vnukov, V. V.; Panina, Svetlana; Krolevets, I. V.; Milutina, N. P.; Ananyan, A. A.; Zabrodin, M. A.; Plotnikov, Andrey A.

doi:10.1134/s2079057015040220

Cited by 4 publications

(5 citation statements)

References 14 publications

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“…Another antioxidant enzyme, glutathione peroxidase catalyzes the reaction between reduced glutathione and hydrogen peroxide [ 7 – 9 ]. In turn, catalase also breaks down hydrogen peroxide to water [ 7 – 10 ]. The sulfhydryl group of thiol compounds, which is involved in detoxification and antioxidant reactions, is recreated with the participation of the enzyme glutathione reductase [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant System and Ions Concentrations After Femoral Neck and Resurfacing Hip Arthroplasty

et al. 2022

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Background Oxidative stress is a disruption of the pro-oxidative-antioxidant balance, caused by excessive production or ineffective removal of reactive oxygen species. Material/Methods The study included 42 male patients aged 38 to 69 years. The first group consisted of 21 men with osteoarthritis after primary hip arthroplasty using the Smith & Nephew Birmingham Hip Resurfacing implant. The second group included 21 men after hip arthroplasty using the femoral neck SPIRON K-implant. In both groups, concentrations of ions, the antioxidant system in the blood, and parameters of oxidative stress were evaluated twice. Clinical assessment using the Western Ontario and McMaster Universities Osteoarthritis Index, Harris Hip Score, and Short Form (12) Health Survey (SF-12) scales was performed. Results Concentrations of metal ions in the blood and the level of oxidative stress were significantly higher in the resurfacing group than in the femoral neck arthroplasty group. The response of the antioxidant system was significantly greater in the femoral neck arthroplasty group. During clinical evaluation, groups did not show significant differences, with the exception of greater shortening of the operated limb and a lower score in the mental-sphere of the SF-12 scale in the resurfacing arthroplasty group. Conclusions Resurfacing hip arthroplasty increased oxidative stress, increased the concentration of metal ions, and did not affect alignment of the abbreviation of the operated limb. A significant improvement in the quality of life of patients in the mental sphere according to the SF-12 occurred after the application of resurfacing arthroplasty, in the first month after the procedure.

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Section: Introductionmentioning

confidence: 99%

Antioxidant System and Ions Concentrations After Femoral Neck and Resurfacing Hip Arthroplasty

et al. 2022

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“…The fruit of T. chebula has been utilized widely in a variety of traditional oriental medicine for the therapy of different diseases. It exhibits significant bioactive effects such as antioxidant and anti-inflammatory activities [18][19][20][21][22][23][24][25][26][27][28][29]. In many studies, T. chebula has been revealed to exhibit antioxidant effects by inhibiting ROS and NO production [21][22][23][24].…”

Section: Discussionmentioning

confidence: 99%

“…Clinical research has also proven that oxidative stress and inflammation contribute to OA, low back pain (LBP), and motor-related joint discomfort. The T. chebula fruit exhibits antioxidant efficacy and downregulates inflammatory cytokines; however, its therapeutic effects warrant further investigation [25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Anti-Osteoarthritic Effects of Terminalia Chebula Fruit Extract (AyuFlex®) in Interleukin-1β-Induced Human Chondrocytes and in Rat Models of Monosodium Iodoacetate (MIA)-Induced Osteoarthritis

Kim

Lee

Lee³

et al. 2020

Applied Sciences

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Osteoarthritis (OA) is a general joint illness caused by the destruction of joint cartilage, and is common in the population of old people. Its occurrence is related to inflammatory reactions and cartilage degradation. AyuFlex® is an aqueous extract of Terminalia chebula fruit, and T. chebula has been utilized extensively in several traditional oriental medications for the management of diverse diseases. Pre-clinical and clinical research has shown its antioxidant and anti-inflammatory effectiveness. Nevertheless, the mechanism underlying the anti-arthritic effects of AyuFlex® remains unclear. In the current research, we proposed the ameliorating effects of AyuFlex® with respect to the incidence of OA and described the latent signalization in interleukin (IL)-1β-treated chondrocytes and MIA-incurred OA in a rat model. In vitro, AyuFlex® decreased oxidative stress and induction of pro-inflammatory cytokines and mediators as well as matrix metalloproteinases (MMPs), while also increasing the levels of collagen synthesis-related proteins. Mechanistically, we identified that AyuFlex® disrupted nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) activation via the inhibition of NF-κB p65 and extracellular regulated protein kinase (ERK) phosphorylation. The ameliorating effects of AyuFlex® were also observed in vivo. AyuFlex® significantly inhibited the MIA-incurred increase in OA symptoms such as oxidative stress, cartilage damage, and changes in cytokines and MMPs revelation in arthrodial cartilage. Therefore, our results suggest that AyuFlex® attenuates OA progression in vivo, indicating that AyuFlex® can be suggested as an excellent therapeutic remedy for the care of OA.

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“…The study shows the importance of oxidative stress and metabolic disturbances in the pathogenesis of osteoarthritis [ 4 ]. The features of regulation of free radical oxidation in the blood and the synovial fluid have been studied in patients with knee osteoarthritis [ 5 ]. Over 90% of diabetic patients are classified as type 2 diabetes cases (T2DM), and one of the primary factors underlying the etiology of T2DM is insulin resistance caused by oxidative stress [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Ginkgo Biloba L. Extract Reduces H2O2-Induced Bone Marrow Mesenchymal Stem Cells Cytotoxicity by Regulating Mitogen-Activated Protein Kinase (MAPK) Signaling Pathways and Oxidative Stress

Wang

Yang

et al. 2018

Med Sci Monit

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BackgroundThe oxidative stress environment of pathological tissue has an adverse effect on the survival of bone marrow mesenchymal stem cells (BMSCs) transplantation. Ginkgo biloba L. extract (EGB) has a potent antioxidant effect. In this research, we assessed the protective effects of EGB and EGB-Containing Serum (EGB CS) on BMSCs against injury induced by hydrogen peroxide (H2O2).Material/MethodsBMSCs were pretreated with EGB or EGB CS and treated with H2O2. The cell counting kit-8 (CCK-8) method was utilized to detect cell viability. The DCFH-DA Fluorescent Kit method was used to detect intracellular ROS level. Malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and (CAT) were determined. The Hoechst staining assay and qRT-PCR assay were utilized to evaluate the effect of EGB on cell apoptosis. Mitogen-activated protein kinases (MAPKs) signaling pathway were detected by western blot analysis.ResultsCompared to the H2O2 group, the number of apoptotic cells in the EGB and EGB CS pretreated groups significantly decreased. The mRNA expression ratio of Bax/Bcl-2 was also decreased. EGB and EGB CS can reduce the production of ROS in BMSCs exposed to H2O2. SOD, GSH-Px and CAT activities were significantly higher compared with those with H2O2 group. Furthermore, EGB or EGB CS pretreatment decreased the protein levels of p-p38MAPK and p-JNK in BMSCs compared to the H2O2 group.ConclusionsOur findings suggested that EGB and EGB CS have protective effect on BMSCs against oxidative stress injury and increase the survival rate of BMSCs transplantation by regulating p38MAPK and JNK signaling.

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Specificities of oxidative stress in the blood and synovial fluid in knee osteoarthritis

Cited by 4 publications

References 14 publications

Antioxidant System and Ions Concentrations After Femoral Neck and Resurfacing Hip Arthroplasty

Antioxidant System and Ions Concentrations After Femoral Neck and Resurfacing Hip Arthroplasty

Anti-Osteoarthritic Effects of Terminalia Chebula Fruit Extract (AyuFlex®) in Interleukin-1β-Induced Human Chondrocytes and in Rat Models of Monosodium Iodoacetate (MIA)-Induced Osteoarthritis

Ginkgo Biloba L. Extract Reduces H2O2-Induced Bone Marrow Mesenchymal Stem Cells Cytotoxicity by Regulating Mitogen-Activated Protein Kinase (MAPK) Signaling Pathways and Oxidative Stress

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