Specification of a Dopaminergic Phenotype from Adult Human Mesenchymal Stem Cells

Trzaska, Katarzyna A.; Kuzhikandathil, Eldo V.; Rameshwar, Pranela

doi:10.1634/stemcells.2007-0212

Cited by 161 publications

(158 citation statements)

References 70 publications

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“…This growth factor cocktail is reported to specifically drive hMSCs to DA neurons. 36,37 A 23 G needle with a suitable plunger fitted to a stereotactic frame was used to deliver the payload of GFP-hMSCs mixed with amyloid hydrogel A5. After transplantation, the system was left undisturbed for 5 min, which assisted the gelation of the matrix.…”

Section: Stem Cell Transplantation In a Parkinsonian Mouse Modelmentioning

confidence: 99%

Implantable amyloid hydrogels for promoting stem cell differentiation to neurons

et al. 2016

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We report a new class of amyloid-inspired peptide hydrogels that was designed and based on α-synuclein protein for which hydrogel formation is triggered by various stimuli, such as heating/cooling or changes in pH. The peptides resemble a cross-β-sheet-rich amyloid, and they assemble into a nanofibrous meshwork that mimics the natural extracellular matrix. Our design principle allows easy manipulation of the gelator sequence to exploit the desirable properties of amyloids for use in cell replacement therapies for neurodegenerative diseases. The amyloid hydrogels facilitate the attachment and neuronal differentiation of mesenchymal stem cells (MSCs) and assist in the delivery and engraftment of MSCs in the substantia nigra and caudate putamen of a Parkinsonian mouse model.

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Section: Stem Cell Transplantation In a Parkinsonian Mouse Modelmentioning

confidence: 99%

Implantable amyloid hydrogels for promoting stem cell differentiation to neurons

et al. 2016

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“…The cells were cultured in a medium that contained osteogenic (10 À7 mol l À1 dexamethasone, 10 mM b-glycerophosphate and 50 mg l À1 ascorbate-phosphate), adipogenic (10 À6 mol l À1 dexamethasone and 10 mg l À1 Insulin) or neurogenic (DMEM/F12 with 0.5% B27, 1% fetal bovine serum, 5% horse serum, 0.5 mM retinoic acid, 20 ng per ml of epidermal growth factor, 50 ng per ml of basic fibroblast growth factor, 50 ng per ml of nerve growth factor) materials. [35][36][37] Regents were mostly ordered from Sigma Aldrich (St Louis, MO, USA), except B27 (Invitrogen), epidermal growth factor and nerve growth factor (Peprotech). Two weeks later, osteogenic differentiation was assessed by Alizarin red staining, and intracellular lipid accumulation was visualized using Oil-Red-O staining and inverted phase contrast microscopy.…”

Section: Isolation and Identification Of Humscsmentioning

confidence: 99%

VEGF-expressing human umbilical cord mesenchymal stem cells, an improved therapy strategy for Parkinson’s disease

et al. 2010

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The umbilical cord provides a rich source of primitive mesenchymal stem cells (human umbilical cord mesenchymal stem cells (HUMSCs)), which have the potential for transplantation-based treatments of Parkinson's Disease (PD). Our pervious study indicated that adenovirus-associated virus-mediated intrastriatal delivery of human vascular endothelial growth factor 165 (VEGF 165) conferred molecular protection to the dopaminergic system. As both VEGF and HUMSCs displayed limited neuroprotection, in this study we investigated whether HUMSCs combined with VEGF expression could offer enhanced neuroprotection. HUMSCs were modified by adenovirus-mediated VEGF gene transfer, and subsequently transplanted into rotenone-lesioned striatum of hemiparkinsonian rats. As a result, HUMSCs differentiated into dopaminergic neuron-like cells on the basis of neuron-specific enolase (NSE) (neuronal marker), glial fibrillary acidic protein (GFAP) (astrocyte marker), nestin (neural stem cell marker) and tyrosine hydroxylase (TH) (dopaminergic marker) expression. Further, VEGF expression significantly enhanced the dopaminergic differentiation of HUMSCs in vivo. HUMSC transplantation ameliorated apomorphine-evoked rotations and reduced the loss of dopaminergic neurons in the lesioned substantia nigra (SNc), which was enhanced significantly by VEGF expression in HUMSCs. These findings present the suitability of HUMSC as a vector for gene therapy and suggest that stem cell engineering with VEGF may improve the transplantation strategy for the treatment of PD.

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“…In this regard, another study demonstrated the importance of factors such as stem cell factor (SCF), produced in situ in the lesioned striatum, to promote the migration and engraftment of MSCs via SCF receptor c-kit (Bantubungi et al, 2008). In the context of PD, several teams, including ours, reported the neuronal differentiation of human MSCs toward a dopaminergic phenotype in vitro, indicating that these cells may constitute an alternative dopamine secreting source of cells (Trzaska & Rameshwar, 2011;Barzilay et al, 2008;Trzaska et al, 2007;). An interesting study also reported no major differences between MSCs from normal patients compared to MSCs isolated from parkinsonian patients, which may be induced to produce up to 30 % of dopaminergic neurons in vitro .…”

Section: Adult Cell Therapymentioning

confidence: 83%

Advances in the Combined Use of Adult Cell Therapy and Scaffolds for Brain Tissue Engineering

Garbayo¹,

Delcroix²,

Schiller³

et al. 2011

Tissue Engineering for Tissue and Organ Regeneration

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Specification of a Dopaminergic Phenotype from Adult Human Mesenchymal Stem Cells

Cited by 161 publications

References 70 publications

Implantable amyloid hydrogels for promoting stem cell differentiation to neurons

Implantable amyloid hydrogels for promoting stem cell differentiation to neurons

VEGF-expressing human umbilical cord mesenchymal stem cells, an improved therapy strategy for Parkinson’s disease

Advances in the Combined Use of Adult Cell Therapy and Scaffolds for Brain Tissue Engineering

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