Advances in the Combined Use of Adult Cell Therapy and Scaffolds for Brain Tissue Engineering

Garbayo, Elisa; Delcroix, Gaëtan J.-R.; Schiller, Paul C.; Montero‐Menei, Claudia N.

doi:10.5772/24250

Cited by 3 publications

(8 citation statements)

References 189 publications

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“…First of all, findings in a number of experimental models showed that neuronal precursor cell survival within the host tissue after transplantation was too weak (10-20%) and that cell death occurred within the first 3 weeks (Delcroix et al, 2010b;Liu and Huang, 2007;Olanow et al, 2003). In situ differentiation of stem cells was insufficient (at best 3-20%) (Delcroix et al, 2010b;Garbayo et al, 2011a). Moreover, cell survival does not depend only on the type of graft or immunosuppressive treatment but also on the site and method of injection of the cells (Modo et al, 2002).…”

Section: Cell Therapy Unanswered Questionsmentioning

confidence: 99%

Organotypic cultures as tools for optimizing central nervous system cell therapies

Daviaud

Garbayo

Schiller

et al. 2013

Experimental Neurology

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Stem cell therapy is a promising treatment for neurological disorders such as cerebral ischemia, Parkinson's disease and Huntington's disease. In recent years, many clinical trials with various cell types have been performed often showing mixed results. Major problems with cell therapies are the limited cell availability and engraftment and the reduced integration of grafted cells into the host tissue. Stem cell-based therapies can provide a limitless source of cells but survival and differentiation remain a drawback. An improved understanding of the behaviour of stem cells and their interaction with the host tissue, upon implantation, is needed to maximize the therapeutic potential of stem cells in neurological disorders. Organotypic cultures made from brain slices from specific brain regions that can be kept in culture for several weeks after injecting molecules or cells represent a remarkable tool to address these issues. This model allows the researcher to monitor/assess the behaviour and responses of both the endogenous as well as the implanted cells and their interaction with the microenvironment leading to cell engraftment. Moreover, organotypic cultures could be useful to partially model the pathological state of a disease in the brain and to study graft-host interactions prior to testing such grafts for pre-clinical applications. Finally, they can be used to test the therapeutic potential of stem cells when combined with scaffolds, or other therapeutic enhancers, among other aspects, needed to develop novel successful therapeutic strategies or improve on existing ones.

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Section: Cell Therapy Unanswered Questionsmentioning

confidence: 99%

Organotypic cultures as tools for optimizing central nervous system cell therapies

Daviaud

Garbayo

Schiller

et al. 2013

Experimental Neurology

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“…When the mode of action is cellular replacement, longevity of transplanted cells in vivo is desired [134][135][136][137][138][139][140][141]. With the majority of replacement cells being adherent, the use of a scaffold as substrate onto which donor cells can attach seems appropriate.…”

Section: Strategies For Long-term Cell Survivalmentioning

confidence: 99%

“…Scaffolds can be made from resorbable and nonresorbable synthetic polymers (e.g., polylactic acid (PLA), polyglycolic acid (PGA), polyethylene glycol (PEG)) or physiological materials (e.g., collagen, gelatin, hyaluronic acid, alginate, fibrin, decellularized extracellular matrix) [135,136]. Molecularly designed biomaterials for use as cell scaffolds can control many of the factors that guide differentiation and function of stem or progenitor cells.…”

Section: Strategies For Long-term Cell Survivalmentioning

confidence: 99%

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Development of Stem Cell Therapy for Medical Uses

Hong¹,

Fung²

2014

Therapeutic Delivery Solutions

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“…To overcome the drawbacks of surgical implantation of 3D scaffolds, minimally invasive implantation methods such as in‐situ gelling systems and scaffold implantation using needle were explored (Garbayo, Montero‐Menei, Delcroix, & Schiller, ). However, these methods were unable to form a spatially organized tissue structure as they required proper gelation condition, pH, temperature, gelling time, and mechanical strength to protect the cells.…”

Section: Introductionmentioning

confidence: 99%

A minimally‐invasive cryogel based approach for the development of human ectopic liver in a mouse model

et al. 2019

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Human liver tissue is preferable over nonhuman liver tissue for preclinical drug screening, as the former can better predict side effects specific to humans. However, due to limited supply and ethical issues with human liver tissue, it is desirable to develop an animal model having functional human liver tissue. In this study, we have established an ectopic functional human liver tissue in a mouse model, using a minimally‐invasive method. Firstly, a human liver tissue mass using HepG2 cells and poly(N‐isopropylacrylamide) (PNIPAAm) incorporated poly(ethylene glycol)‐alginate‐gelatin (PAG) cryogel matrix was developed in vitro. It was later implanted in mouse peritoneal cavity using a 16 G needle. Viscoelastic nature along with low Young's modulus provided injectable properties to the cryogel. We confirmed minimal cell loss/death while injecting. Further, by in vivo study efficacy of both injectable and surgical implantation approaches were compared. No significant difference in terms of cell infiltration, human serum albumin (HSA) secretion and enzyme activity confirmed efficacy. This model developed using a minimally‐invasive approach can overcome the limitations of surgical implantation due to its cost effective and user friendly nature.

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Advances in the Combined Use of Adult Cell Therapy and Scaffolds for Brain Tissue Engineering

Cited by 3 publications

References 189 publications

Organotypic cultures as tools for optimizing central nervous system cell therapies

Organotypic cultures as tools for optimizing central nervous system cell therapies

Development of Stem Cell Therapy for Medical Uses

A minimally‐invasive cryogel based approach for the development of human ectopic liver in a mouse model

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