Specific mutations in the genes of MC1R and TYR have an important influence on the determination of pheomelanin pigmentation in Korean native chickens

Nam, In Sik; Oh, Min-Gee; Nam, Myoung Soo; Kim, Woan Sub

doi:10.5455/javar.2021.h511

Cited by 4 publications

(4 citation statements)

References 17 publications

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“…In our prior experiment, the expression level of MC1R mRNA in skin tissue was higher in black quails than in Beijing white quails (Zhang et al, 2013). The same phenomenon was found in Korean native chickens (Nam et al, 2021) and Silky Fowl chickens (Li et al, 2011). In this study, the expression of MC1R mRNA had no significant difference between individuals, which indicated that the differences of total melanin and pheomelanin in feathers were not caused by the expression of the MC1R gene, but by the mutation in the MC1R gene.…”

Section: Discussionsupporting

confidence: 73%

“…Variation in the E locus was an important factor in determining plumage color in chickens (Yang et al, 2019) and some mammalian species (Gebreselassie et al, 2020, Jiang et al, 2021. The mutations in the MC1R gene could affect various feather colors in Korean native chicken breeds (Nam et al, 2021). The same phenomena have been reported in Chinese native chicken breeds (Yang et al, 2019, Zhang et al, 2020, native Japanese chicken (Kabir et al, 2020), blackbone chicken (Khumpeerawat et al, 2021), and so on.…”

Section: Discussionmentioning

confidence: 68%

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Identification of Polymorphism in the MC1R Gene and Its Association with the Melanin Content in Feathers of Chinese Yellow Quails

Zhang

Pang

et al. 2023

Braz. J. Poult. Sci.

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MC1R plays a crucial role in controlling the type of melanin synthesized in the melanocytes, which greatly affects plumage color in birds. One g.16796362G/T SNP was found in the MC1R gene coding region, which caused a Met120Ile mutation in the amino acid sequence. The Met120Ile mutation was located in the third transmembrane domain of the MC1R protein and decreased protein stability. The g.16796362G/T locus achieved medium polymorphism and had significant association with feather melanin content in Chinese yellow quails. The contents of total melanin and pheomelanin with AA genotype were significant lower than those with AB or BB genotypes in skin tissues, while the expression levels of MC1R mRNA had no significant difference in feathers with different genotypes. This experiment indicated that the Met120Ile mutation could affect the function of the MC1R protein and change the biosynthesis of melanin in Chinese yellow quails.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 68%

Identification of Polymorphism in the MC1R Gene and Its Association with the Melanin Content in Feathers of Chinese Yellow Quails

Zhang

Pang

et al. 2023

Braz. J. Poult. Sci.

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“…The acquired bioactive compounds in SCE were subjected to in silico molecular docking against melanogenesis-related proteins tyrosinase (TYR, PDB ID: 2Y9X) and melanin-concentrating hormone receptor 1 (MC1R, PDB ID: 7F41) [ 67 ]. From 33 acquired bioactive compounds from LCMS UHPLC-HR-ESI-MS analyses, 27 compounds were selected as ligands based on molecular weight (<500 Da).…”

Section: Methodsmentioning

confidence: 99%

Brown Macroalgae Sargassum cristaefolium Extract Inhibits Melanin Production and Cellular Oxygen Stress in B16F10 Melanoma Cells

et al. 2022

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The brown macroalgae Sargassum has been reported for its anti-UV and photoprotective potential for industrial applications. This study evaluated the melanin inhibition activity of Sargassum cristaefolium (SCE) ethanol extract. Melanogenesis inhibition by SCE was assessed in vitro with B16-F10 melanoma cell models and in silico against melanin regulatory proteins Tyrosinase (TYR) and Melanocortin 1 Receptor (MC1R). The regulatory properties evaluated were the melanin content, intracellular tyrosinase activity and cellular antioxidant activities. In addition, the bioactive compounds detected in SCE were subjected to molecular docking against TYR and MC1R. Based on the results, 150 µg/mL SCE effectively inhibited the production of melanin content and intracellular tyrosinase activity. Cellular tyrosinase activity was reduced by SCE-treated cells in a concentration-dependent manner. The results were comparable to the standard tyrosinase inhibitor kojic acid. In addition, SCE effectively decreased the intracellular reactive oxygen species (ROS) levels in B16-F10 cells. The antioxidant properties may also contribute to the inhibition of melanogenesis. In addition, LCMS UHPLC-HR-ESI-MS profiling detected 33 major compounds. The results based on in silico study revealed that the bioactive compound putative kaurenoic acid showed a strong binding affinity against TYR (−6.5 kcal/mol) and MC1R (−8.6 kcal/mol). However, further molecular analyses are needed to confirm the mechanism of SCE on melanin inhibition. Nevertheless, SCE is proposed as an anti-melanogenic and antioxidant agent, which could be further developed into cosmetic skin care products.

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“…Melanin is divided into eumelanin and pheomelanin. As the main component of avian plumage color formation, melanin deposition and degradation will have an important impact on the formation of plumage color (Nam et al, 2021;Li et al, 2012). Melanin synthesis is mainly by amino acid synthesis of tyrosine in the body, through the tyrosinase catalytic body tyrosine hydroxylation and a series of biochemical reactions (Raghunath et al, 2015;Galeb et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Association analysis of melanophilin (MLPH) gene expression and polymorphism with plumage color in quail

et al. 2023

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Abstract. We explore the relationship between the melanophilin (MLPH) gene and quail plumage color and provide a reference for subsequent quail plumage color breeding. In this experiment, real-time quantitative PCR (RT-qPCR) technology was used to analyze the relative mRNA expression levels of Korean quail (maroon) and Beijing white quail embryos at different developmental stages. Two single-nucleotide polymorphisms (SNPs) in the MLPH gene were screened based on the RNA-sequencing (RNA-Seq) data of skin tissues of Korean quail and Beijing white quail during the embryonic stage. Kompetitive allele-specific PCR (KASP) technology was used for genotyping in the resource population, and correlation analysis was carried out with the plumage color traits of quail. Finally, bioinformatics was used to predict the effects of these two SNPs on the structure and function of the encoded protein. The results showed that the expression level of the MLPH gene during embryonic development of Beijing white quail was significantly higher than that of Korean quail (P<0.01). The frequency distribution of the three genotypes (CC, CA and AA) of the Beijing white quail at the c.1807C > A mutation site was significantly different from that of the Korean quail (P<0.01). The frequency distribution of the three genotypes (GG, GA and AA) of the Beijing white quail at the c.2129G > A mutation site was significantly different from that of the Korean quail (P<0.01). And there was a significant correlation between the c.1807C > A mutation site and the white plumage phenotype. Bioinformatics showed that SNP1 (c.1807C > A) was a neutral mutation and that SNP2 (c.2129G > A) was a deleterious mutation. The prediction of protein conservation showed that the mutation sites of coding proteins R603S and G710D caused by SNP1 (c.1807C > A) and SNP2 (c.2129G > A) were highly conserved.

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Specific mutations in the genes of MC1R and TYR have an important influence on the determination of pheomelanin pigmentation in Korean native chickens

Cited by 4 publications

References 17 publications

Identification of Polymorphism in the MC1R Gene and Its Association with the Melanin Content in Feathers of Chinese Yellow Quails

Identification of Polymorphism in the MC1R Gene and Its Association with the Melanin Content in Feathers of Chinese Yellow Quails

Brown Macroalgae Sargassum cristaefolium Extract Inhibits Melanin Production and Cellular Oxygen Stress in B16F10 Melanoma Cells

Association analysis of melanophilin (MLPH) gene expression and polymorphism with plumage color in quail

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