Specific IgG subclass antibody in rubella virus infections

Thomas, H. I. J.; Morgan‐Capner, P.

doi:10.1017/s0950268800067182

Cited by 30 publications

(20 citation statements)

References 16 publications

(14 reference statements)

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“…7-DISCUSSION The adaptation to measure avidity of the antiglobulin ELISA for rubellaspecific IgG subclass antibody was straightforward, using the protein denaturant diethylamine as described by Devey et al (1988). We examined the avidity of specific IgG1 and IgG3 separately, as we had recently developed these assays and had demonstrated their validity (Thomas & Morgan-Capner, 1988). However, the method described here should be readily adaptable to an antiglobulin ELISA for total rubella-specific IgG or antibody, and we have confirmed this with a few sera.…”

Section: Specific Igg1 Shift Valuesmentioning

confidence: 54%

“…All contained rubella-specific IgG0 at a concentration > 30 arbitrary units (a.u.) (Thomas & Morgan-Capner, 1988).…”

Section: Seramentioning

confidence: 99%

“…Isotype-specific avidity ELISA Microtitre plates were coated with rubella antigen as described previously (Thomas & Morgan-Capner, 1988). The treatment of serum with the protein denaturant diethylamine (DEA) was an adaptation of the method described by Devey et al (1988).…”

Section: Seramentioning

confidence: 99%

“…The plates were incubated in a sealed moist box for 1 h at room temperature before washing six times with PBST. The remainder of the assay (monoclonal anti-IgG subclass antibodies, peroxidase-conjugated anti-mouse immunoglobulin and substrate) was carried out as described previously (Thomas & Morgan-Capner, 1988). The reaction was stopped by the addition of 4N-H2SO4before the maximum optical density (OD) reached 2'00.…”

Section: Seramentioning

confidence: 99%

“…However, the sera had unusually high concentrations of specific IgG3 (both 3 100 a.u.) for reinfection (Thomas & Morgan-Capner, 1988). No pre-contact or earlier serum was available, but the patient had been immunized against rubella 9 years previously.…”

Section: Specific Igg1 Shift Valuesmentioning

confidence: 99%

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Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

Thomas

Morgan‐Capner

1988

Epidemiol. Infect.

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SUMMARYAn antiglobulin enzyme-linked immunosorbent assay for rubella-specific IgG1 and IgG3 was adapted to measure antibody avidity by incorporating a mild protein denaturant, diethylamine (DEA), into the serum diluent. Sera were tested at varying dilutions, both with and without DEA, if they contained sufficient specific IgG, or IgG3. The optical density (OD) was measured and curves were plotted. The highest OD (V) was noted and halved (V/2). The distance between the OD curves at V/2 was measured as the DEA shift value.Sera were examined from people whose sera contained rubella-specific antibodies as a consequence of infection or vaccination in the distant past (24 sera), recent primary rubella (66 sera), symptomatic reinfection (11 sera) or asymptomatic reinfection (64 sera). For specific IgG1 the DEA shift value was < 0W6 for cases of rubella in the distant past, compared with > 0-8 for the first month after primary infection. The maximum DEA shift value for the sera from cases of reinfection was 0-65.No serum from cases of rubella in the distant past contained sufficient specific IgG3 to estimate avidity. The sera collected within 1 month of onset of primary rubella gave DEA shift values > 0 7 compared with sera from reinfections, which gave DEA shift values < 0 6, except for two sera from a case of symptomatic reinfection.Thus the assessment of specific IgG subclass avidity is of value in differentiating serologically primary rubella from reinfection.

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Section: Specific Igg1 Shift Valuesmentioning

confidence: 54%

“…All contained rubella-specific IgG0 at a concentration > 30 arbitrary units (a.u.) (Thomas & Morgan-Capner, 1988).…”

Section: Seramentioning

confidence: 99%

Section: Seramentioning

confidence: 99%

Section: Seramentioning

confidence: 99%

Section: Specific Igg1 Shift Valuesmentioning

confidence: 99%

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Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

Thomas

Morgan‐Capner

1988

Epidemiol. Infect.

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Persistent rubella‐specific IgM reactivity in the absence of recent primary rubella and rubella reinfection

Thomas

Morgan‐Capner

Roberts

et al. 1992

Journal of Medical Virology

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Between two and seven sera from cases of persistent detection of rubella-specific IgM for periods in excess of 2.5 months, but in the absence of recent primary rubella or rubella reinfection, were examined for rheumatoid factor, heterophile antibody, and IgM reactivity against toxoplasma and a number of viruses. The relative avidity of the rubella-specific IgG1 has been assessed in all the sera by two methods. None of the sera contained rheumatoid factor or heterophile antibody, nor did any contain detectable concentrations of IgM specific for any of the panel of antigens apart from five sera which contained low concentrations of IgM specific for some coxsackieviruses B. No sera were positive for low avidity specific IgG1 although three did give equivocal results with one avidity test and one gave equivocal results with the second avidity test.

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Low antibody avidity in elderly chickenpox patients

Schoub

Blackburn

Johnson

et al. 1992

Journal of Medical Virology

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A small outbreak of chickenpox confirmed serologically in 3 elderly patients from a geriatric home is described. Disease was probably due to exogenous reinfection, yet nevertheless the avidity of specific antibodies measured by the urea denaturation test was even lower than in primary chickenpox controls, which themselves were, as expected, significantly lower than zoster controls. In elderly individuals susceptibility to reinfection with varicella-zoster virus (VZV) with clinical manifestation such as chickenpox may well be associated with the decay of specific humoral immunity detectable by antibodies of particularly low avidity, in contrast to reactivation of latent VZV presenting clinically as zoster, which is related to deficiencies in specific cellular immunity.

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Specific IgG subclass antibody in rubella virus infections

Cited by 30 publications

References 16 publications

Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

Persistent rubella‐specific IgM reactivity in the absence of recent primary rubella and rubella reinfection

Low antibody avidity in elderly chickenpox patients

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