Specific enzyme activities in bronchoalveolar lavage fluid as an aid to diagnosis of tracheobronchitis and bronchopneumonia in dogs

Maden, Mehmet; Altunok, Vahdettin; Birdane, Fatih Mehmet; Aslan, Veysi; Nizamlıoğlu, Mustafa

doi:10.1053/rvsc.2001.0503

Cited by 13 publications

(20 citation statements)

References 24 publications

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“…The present study demonstrated the effect of chrysin on ALP (toxic damage or proliferation of pneumocytes type II), LDH (increased membrane permeability and cell lysis) and also checked the TP level (altered respiratory membrane and vascular permeability) (Maden et al, 2001). Tissue concentration of LDH enzyme activity is about 500 times higher than those normally found in serum (Drent et al, 1996).…”

Section: Discussionsupporting

confidence: 57%

Anti-asthmatic potential of chrysin on ovalbumin-induced bronchoalveolar hyperresponsiveness in rats

Wadibhasme

Ghaisas

Thakurdesai

2011

Pharmaceutical Biology

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Section: Discussionsupporting

confidence: 57%

Anti-asthmatic potential of chrysin on ovalbumin-induced bronchoalveolar hyperresponsiveness in rats

Wadibhasme

Ghaisas

Thakurdesai

2011

Pharmaceutical Biology

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“…The BAL fluid samples were evaluated cytologically and enzymologically 21,22 . The cytological differences and differential cell numbers were defined using two different staining techniques (WrightGiemsa and Papanicolaou).…”

Section: Analysis Of Bal Fluidmentioning

confidence: 99%

“…The increase in the enzyme activities in the BAL fluid (LDH, AST and GGT) reflects cell injury 11,21,22 . In this study, there were significant differences in the LDH and GGT enzyme activities of the BAL fluid between groups (Table 5).…”

mentioning

confidence: 99%

“…Many studies 12,22,31 have shown that the BAL fluid protein and urea measurements in lung diseases can show vascular and respiratory permeability defects. In this study, when the increases in the enzyme activities were considered, the changes found in the MP and urea levels confirm the existence of respiratory and vascular permeability deformities and pulmonary damage.…”

mentioning

confidence: 99%

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Cytologic-Enzymologic Diagnosis of Experimental Pneumonia Induced by Klebsiella pneumoniae Serotype II in Rats and Its Treatment with Free and Liposomal Enrofloxacin

Baş¹,

Üney²,

Hadimli³

et al. 2013

Kafkas Univ Vet Fak Derg

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SummaryEnrofloxacin (ENR) rapidly localizes in eukaryotic cells in vitro but does not remain for prolonged periods, thereby reducing the ENR efficacy of defense against intracellular pathogens. Delivery of ENR in a liposome-encapsulated form may enhance its intracellular residence time. In this study, experimental pneumonia was induced in healthy and dexamethasone-treated rats using Klebsiella pneumoniae serotype II. Free and liposome-encapsulated ENR were injected intravenously into the infected animals at a dose of 7.5 mg/kg/day for 5 days. Samples of tissue, plasma and bronchoalveolar lavage (BAL) fluid were obtained at 1, 2, 3 and 4 days and 1, 2, 3 and 4 weeks after the first antibiotic treatment. All of the samples were evaluated cytologically, enzymologically, microbiologically and pathologically. It was determined that cytologic and enzymologic diagnoses of BAL fluid are not meaningful for evaluating the treatment of the experimental pneumonia in rats. However, it was established that the use of ENR in liposomal form at a dose of 7.5 mg/kg for 5 days is more effective than the free form both in the treatment of K. pneumoniae infections and in the prevention of recurrent infections. Liposome-encapsulated antimicrobial agents should provide another choice for antimicrobial therapy in the future, but further investigation must be completed before clinical use. Keywords: Enrofloxacin, Experimental pneumonia, Klebsiella pneumoniae, Liposome, Rat, Treatment Ratlarda Klebsiella pneumoniae Serotip II İle Oluşturulan Deneysel Pnömonilerin Sitolojik-Enzimolojik Teşhisi ve Serbest ve Lipozomlanmış Enrofloksasin İle TedavisiÖzet Enrofloksasin (ENR) in vitro ortamlarda ökaryotik hücrelerde hızla lokalize olur, ancak uzun süre kalamadığı için hücre içi patojenlere karşı onun etkinliği azalır. Lipozomla kapsüle edilmiş formda ENR uygulanması hücre içi ortamlarda kalış zamanını artırabilir. Bu çalışmada, sağlıklı ve deksametazon uygulanmış ratlarda Klebsiella pneumoniae serotip II kullanılarak deneysel pnömoni oluşturuldu. Serbest ve lipozomla kapsüle edilmiş ENR enfekte hayvanlara intravenöz olarak beş gün boyunca 7.5 mg/kg/gün dozda enjekte edildi. İlk antibiyotik uygulamasından sonraki 1, 2, 3, 4. gün ve 1, 2, 3, 4. haftalarda plazma, doku ve bronko-alveolar lavaj (BAL) sıvısı örnekleri alındı. Tüm örnekler sitolojik, enzimolojik, mikrobiyolojik ve patolojik olarak değerlendirildi. Ratlarda deneysel pnömoninin tedavisinin değerlendirilmesinde BAL sıvısının sitolojik ve enzimolojik teşhisinin anlamsız olduğu belirlendi. Ancak, enrofloksasinin beş gün boyunca 7.5 mg/kg dozda lipozomal formda kullanımının hem K. pneumoniae enfeksiyonlarının tedavisinde hem de tekrarlayan enfeksiyonlarının önlenmesinde serbest formdan daha etkili olduğu tespit edildi. Lipozomla kapsüle edilmiş antimikrobiyal ajanlar gelecekte antimikrobiyal tedavide diğer bir seçenek sağlayacaklardır, ancak klinik kullanımdan önce çok sayıda araştırma yapılmalıdır.

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“…Essa atividade parece estar relacionada com a síntese de compostos fosfolipídicos e proteicos da substância surfactante pulmonar (KALINA et al, 1990). Pesquisadores já demonstraram a importância clínica da utilização da determinação da atividade da FAL no trato respiratório posterior como ferramenta diagnóstica em várias espécies (HENDERSON et al, 1995;CAPELLI et al, 1997;COBBEN et al, 1999;MADEN et al, 2001).…”

Section: Introductionunclassified

Atividade da fosfatase alcalina no lavado broncoalveolar de equinos de policiamento montado no Estado do Rio de Janeiro

et al. 2014

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Specific enzyme activities in bronchoalveolar lavage fluid as an aid to diagnosis of tracheobronchitis and bronchopneumonia in dogs

Cited by 13 publications

References 24 publications

Anti-asthmatic potential of chrysin on ovalbumin-induced bronchoalveolar hyperresponsiveness in rats

Anti-asthmatic potential of chrysin on ovalbumin-induced bronchoalveolar hyperresponsiveness in rats

Cytologic-Enzymologic Diagnosis of Experimental Pneumonia Induced by Klebsiella pneumoniae Serotype II in Rats and Its Treatment with Free and Liposomal Enrofloxacin

Atividade da fosfatase alcalina no lavado broncoalveolar de equinos de policiamento montado no Estado do Rio de Janeiro

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