Specific antibody detection in serum, urine and saliva samples for the diagnosis of cystic echinococcosis

Sunita, T.; Dubey, M L; Khurana, Sumeeta; Malla, Nancy

doi:10.1016/j.actatropica.2006.07.014

Cited by 25 publications

(25 citation statements)

References 12 publications

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“…There was no significant difference among other IgG isotypes (IgG1, IgG2 and IgG3). Previous studies have also reported higher diagnostic sensitivity of total IgG, IgE and IgG4 (Khabiri et al, 2006;Sunita et al, 2007). Recent studies on IgG subclass responses in human CE have demonstrated IgG1 and IgG4 to be the most immunodominant IgG isotypes (Hira et al, 1990;Aceti et al, 1993;Wen & Craig, 1994;Zhang et al, 2003).…”

Section: Discussionmentioning

confidence: 90%

Detection of hydatid-specific antibodies in the serum and urine for the diagnosis of cystic echinococcosis in patients from the Kashmir Valley, India

Chirag¹,

Fomda²,

Khan³

et al. 2014

J. Helminthol.

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Serological diagnosis of cystic echinococcosis (CE) is usually made by detecting specific antibodies in serum samples. However, collection of blood samples is difficult and may be hazardous and unsafe. Thus, it is important to assess alternative simple methods of sampling body fluids that give similar results. Saliva and urine have been suggested as possible alternatives to detect specific antibodies for the diagnosis of various diseases. To the best of our knowledge, there has been no previously published study regarding the detection of CE-specific immunoglobulin (Ig) G subclass antibodies (IgG1-4) in urine. Therefore, the present study was designed to assess the value of hydatid-specific antibodies of IgG, IgM, IgE and IgG subclass in urine and serum samples for the diagnosis of CE. Serum and urine samples of 41 surgically confirmed patients of CE, 40 patients with other diseases and 16 healthy subjects were included in the study. CE-specific total IgG, IgE and IgG4 in sera and total IgG, IgG4 and IgG1 in the urine of CE patients were the most important specific antibodies for the diagnosis of CE. However, total IgG usually persists for an extended period and has a very high cross-reactivity. The diagnostic sensitivity of hydatid-specific IgM in serum and urine samples was very low and therefore cannot be used as a diagnostic marker. There was no significant difference between IgG1 and IgG4 in serum and urine and both showed the best correlation for the diagnosis of CE. These considerations suggest that detection of antibodies in urine could provide a new approach in the diagnosis of CE.

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Section: Discussionmentioning

confidence: 90%

Detection of hydatid-specific antibodies in the serum and urine for the diagnosis of cystic echinococcosis in patients from the Kashmir Valley, India

Chirag¹,

Fomda²,

Khan³

et al. 2014

J. Helminthol.

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“…Saliva reflects serum immune response and salivary immunoglobulins are good candidates for helminthiasis diagnosis such as opisthorchiasis [17], schistosomiasis japonica [34], fascioliosis [35], cystic echinoccosis [16] and neurocysticercosis [14].…”

Section: Discussionmentioning

confidence: 99%

“…Thus, it is important to assess alternative, simple and painless methods of sampling body fluids that give results as accurate as those obtained with serum samples. Saliva has been suggested as a possible alternative and is increasingly being reported as a non-invasive means to detect specific antibodies to diagnose various diseases because salivary antibodies can reflect both secretory and systemic immune responses [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Detergent fraction of heterologous antigen to detect IgA and IgG in strongyloidiasis using saliva and serum paired samples

et al. 2010

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Strongyloides stercoralis causes chronic asymptomatic infections in immunocompetent human hosts and systemic invasion in immunocompromised patients, developing into a fatal hyperinfection syndrome. IgA and IgG detection in saliva and serum paired samples were tested using total saline extract from Strongyloides venezuelensis (SE(*)) and its detergent phase (D) extracted with Triton X-114. Saliva and serum paired samples were obtained from: 25 patients with confirmed strongyloidiasis; 25 patients with other parasitoses and 20 from apparently healthy individuals. Sensitivity, specificity, diagnostic efficiency, positive and negative predictive values and likelihood ratio were calculated at the optimum point of reaction. Using D phase sensitivity and specificity to detect IgA in saliva were 76.0% and 88.9% and in serum 80.0% and 86.7%, respectively. To detect IgG, D phase showed sensitivity and specificity of 88.0% and 88.9% in saliva and 88.0% and 84.4% in serum, respectively. D phase proved to be specific and efficient and could be utilized as an alternative antigen for IgA and IgG detection in saliva and serum samples for strongyloidiasis diagnosis.

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“…Routine laboratory diagnosis of CE is dependent on detection of specific antibody response. Serum is generally used for detection of specific antibody although some studies show the detection of antibody in urine might also be a good alternative [18]. The prevalence of CE in human in areas of extensive farming reaches up to 5% [19].…”

Section: Discussionmentioning

confidence: 99%

Field Investigation on Cystic Hydatid Infection in Man and Camel in Tambool Town and Khartoum State Hospitals, Sudan

Albadawi

Ahmed²,

Osman³

et al. 2016

J Bacteriol Parasitol

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Echinococcosis/hydatidosis is a zoonotic disease. The disease is caused by adult worms and larval (metacestode) stages of the taeniid cestode Echinococcus granulosus. The life cycle is completed into two hosts. The final host usually carnivore e.g. dog, and the intermediate host usually herbivorous and man. The aim of this study is to evaluate the fertility of cysts and viability of protoscoleces in man and camel hydatid cysts in order to determine the prevalence of their fertility and to study the association with variables typical of host and of the parasite. Material and methods:Across-sectional and observations study was done in biological material (the fluid from human and camel hepatic and lung hydatid cysts). The viability criteria used were ovoid form invaginated scolices and intact calcareous corpuscles. The presence of vibrating movements, and the absence of "vital" staining, the cysts were grouped in diameter cysts from small cysts to very large size of cysts. Descriptive statistics were for the calculation of the prevalence of fertility, analytical statistics for comparison of groups, and multivariate analysis for examination of the association between cyst fertility and clinical variables. Results:A total of 99 cysts with less than 2 cm 5 (7.8%), from 2-6 cm 52 (81.2%,) medium diameter 6-10 cm 37 (57.8%), and more than 10 cm 5 (7.8), and prevalence of general fertility 68%, sterial cysts was 6%, supporative was 10% and calcified was 15%. Most of camel fertile cysts had medium size (81.2%), while the lowest rate belongs to the small cysts (7.8%). There was significant relationship between fertility of the cyst, type of infected organs, type of animals and size of cyst. Association with location, type, and diameter of the cyst, the viability Protoscoleces was assessed by microscopic observation of biological feature represented by muscular movements, flame cell activity in 0.1% aqueous eosin stain. Conclusion:The differences between prevalence rates, the fertility of hydatid cysts and diversity sites localization observed in humans and camels. Fertility is associated with the type of cyst, location type, type of animal and size of cyst.

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Specific antibody detection in serum, urine and saliva samples for the diagnosis of cystic echinococcosis

Cited by 25 publications

References 12 publications

Detection of hydatid-specific antibodies in the serum and urine for the diagnosis of cystic echinococcosis in patients from the Kashmir Valley, India

Detection of hydatid-specific antibodies in the serum and urine for the diagnosis of cystic echinococcosis in patients from the Kashmir Valley, India

Detergent fraction of heterologous antigen to detect IgA and IgG in strongyloidiasis using saliva and serum paired samples

Field Investigation on Cystic Hydatid Infection in Man and Camel in Tambool Town and Khartoum State Hospitals, Sudan

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