Specific and heritable gene editing in
            <i>Arabidopsis</i>

Gao, Yangbin; Zhao, Yunde

doi:10.1073/pnas.1402295111

Cited by 31 publications

(20 citation statements)

References 13 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results, along with those of others Gao and Zhao, 2014;Zhang et al, 2014), suggest that the CRISPR/Cas9 system is poised to rapidly become the technology of choice to generate gene knockouts for reverse genetics studies. As an example, we recently discovered using forward genetics that the gene Solyc11g064850 controls multiple aspects of tomato reproductive development (K. Liberatore, C. MacAlister, and Z.B.…”

supporting

confidence: 67%

“…In the animal field, the CRISPR/ Cas9 system was reported to have a high off-target rate (Carroll, 2013). In contrast, several recent studies have shown high specificity of CRISPR/Cas9 in plants Gao and Zhao, 2014;Zhang et al, 2014). The issue of off targeting in plants still needs to be addressed systematically, but several approaches can minimize possible impacts, such as using a recently developed algorithm for plant genomes that selects CRISPR/Cas9 sgRNAs with the least predicted off targets (Xie et al, 2014).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System

et al. 2014

View full text Add to dashboard Cite

supporting

confidence: 67%

mentioning

confidence: 99%

Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System

et al. 2014

View full text Add to dashboard Cite

“…In many species, it has therefore been possible to segregate the transgene locus and the target locus in the progeny (or T1) generation of at least some transgenic events, resulting in transgene-free plants with mutations at the desired target. This has been demonstrated in many species, for example Arabidopsis thaliana (Arabidopsis) [11], Oryza sativa (rice) [12], Hordeum vulgare (barley) [13] and Brassica oleracea [13].…”

Section: Introductionmentioning

confidence: 92%

CRISPR-based tools for plant genome engineering

Silva

Patron

2017

Emerging Topics in Life Sciences

View full text Add to dashboard Cite

Molecular tools adapted from bacterial CRISPR (clustered regulatory interspaced short palindromic repeat) adaptive immune systems have been demonstrated in an increasingly wide range of plant species. They have been applied for the induction of targeted mutations in one or more genes as well as for directing the integration of new DNA to specific genomic loci. The construction of molecular tools for multiplexed CRISPR-mediated editing in plants has been facilitated by cloning techniques that allow multiple sequences to be assembled together in a single cloning reaction. Modifications of the canonical Cas9 protein from Streptococcus pyogenes and the use of nucleases from other bacteria have increased the diversity of genomic sequences that can be targeted and allow the delivery of protein cargos such as transcriptional activators and repressors. Furthermore, the direct delivery of protein-RNA complexes to plant cells and tissues has enabled the production of engineered plants without the delivery or genomic integration of foreign DNA. Here, we review toolkits derived from bacterial CRISPR systems for targeted mutagenesis, gene delivery and modulation of gene expression in plants, focusing on their composition and the strategies employed to reprogramme them for the recognition of specific genomic targets.

show abstract

“…This allows creation of nontransgenic plants and improved crop varieties [22,[91][92][93]. These technologies are faster compared to traditional breeding methods and help to obtain the null segregant lines that have lost the transgene insertion [94][95][96][97].…”

Section: Regulation Of Plants Created By Genome Editingmentioning

confidence: 99%

Genome Editing in Plants: An Overview of Tools and Applications

Kamburova

Никитина

Shermatov

et al. 2017

International Journal of Agronomy

View full text Add to dashboard Cite

The emergence of genome manipulation methods promises a real revolution in biotechnology and genetic engineering. Targeted editing of the genomes of living organisms not only permits investigations into the understanding of the fundamental basis of biological systems but also allows addressing a wide range of goals towards improving productivity and quality of crops. This includes the creation of plants with valuable compositional properties and with traits that confer resistance to various biotic and abiotic stresses. During the past few years, several novel genome editing systems have been developed; these include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9). These exciting new methods, briefly reviewed herein, have proved themselves as effective and reliable tools for the genetic improvement of plants.

show abstract

Specific and heritable gene editing in Arabidopsis

Cited by 31 publications

References 13 publications

Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System

Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System

CRISPR-based tools for plant genome engineering

Genome Editing in Plants: An Overview of Tools and Applications

Contact Info

Product

Resources

About