Species Differences in in vitro and Estimated in vivo Kinetics for Intestinal Microbiota Mediated Metabolism of Acetyl‐deoxynivalenols

Jin, Jing; Spenkelink, A.; Beekmann, Karsten; Baccaro, Marta; Xing, Fuguo; Rietjens, Ivonne M.C.M.

doi:10.1002/mnfr.202001085

Cited by 5 publications

(12 citation statements)

References 26 publications

(45 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Under the conditions applied, the conversion of 13-Ac-DON and 15-Ac-DON by human fecal samples had already been shown previously to be linear in time and with the amount of fecal sample [ 11 ], enabling in vivo estimation of the corresponding conversion rates. A time period of 4 h was chosen to quantify potential inter-human differences in gut microbial metabolism of 3-Ac-DON and 15-Ac-DON.…”

Section: Resultsmentioning

confidence: 99%

“…The aim of this study was to assess the interindividual differences in the intestinal microbial transformation of 3-Ac-DON and 15-Ac-DON to DON using in vitro anaerobic fecal incubations. Acetylated DONs are major modified forms of DON present in food and feed, and due to their deacetylation by human intestinal microbiota [ 11 ], the exposure of acetylated DONs adds to the overall DON exposure. Assuming even distribution of a dose level over the large intestinal content of 2.06 L [ 16 ], an overall concentration of 200 µM would result from a dose level of 1991 µg kg −1 b.w.…”

Section: Discussionmentioning

confidence: 99%

“…The observation of conversion of acetylated DONs to DON by intestinal microbiota is in accordance with previous reports. In our previous study [ 11 ], deacetylation of 3-Ac-DON and 15-Ac-DON by a pooled human fecal sample from 10 healthy volunteers was reported. Based on deconjugation rates obtained with this pooled human fecal sample it was concluded that 3-Ac-DON and 15-Ac-DON can be fully deconjugated upon intestinal transit.…”

Section: Discussionmentioning

confidence: 99%

Section: Estimation Of Microbial Transformation Of 3-ac-don and 15-ac...mentioning

confidence: 90%

“…Previous studies have shown that the gut microbiota of different host species, such as cattle, pig, chicken, and humans, can uncouple modified DON from DON, resulting in increased exposure of DON [ 2 , 11 ]. Young et al [ 12 ] proved that microbial bacteria and pure cultures from chicken bowel can degrade two acetylated DONs and DON by the way of deacetylation and/or oxidation.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Interindividual Differences in In Vitro Human Intestinal Microbial Conversion of 3-Acetyl-DON and 15-Acetyl-DON

Jin

Rietjens

et al. 2022

Toxins

Self Cite

View full text Add to dashboard Cite

In order to evaluate the potential differences between 3-Ac-DON and 15-Ac-DON in the human intestinal microbial metabolism, human fecal samples were anaerobically cultured in vitro. Quantitative fecal microbiota characteristics were obtained by 16S rRNA sequencing, and the data revealed several genera that may be relevant for the transformation of the acetylated DONs. Significant differences in the level of 3-Ac-DON and 15-Ac-DON conversion were observed among microbiota from different human individuals. 3-Ac-DON could be rapidly hydrolyzed; a ten-fold difference was observed between the highest and lowest in vitro conversion after 4 h. However, 15-Ac-DON was not fully transformed in the 4 h culture of all the individual samples. In all cases, the conversion rate of 3-Ac-DON was higher than that of 15-Ac-DON, and the conversion rate of 3-Ac-DON into DON varied from 1.3- to 8.4-fold that of 15-Ac-DON. Based on in vitro conversion rates, it was estimated that 45–452 min is required to convert all 3-Ac-DON to DON, implying that deacetylation of 3-Ac-DON is likely to occur completely in all human individuals during intestinal transit. However, for conversion of 15-Ac-DON, DON formation was undetectable at 4 h incubation in 8 out of the 25 human samples, while for 7 of these 8 samples conversion to DON was detected at 24 h incubation. The conversion rates obtained for these seven samples indicated that it would take 1925–4805 min to convert all 15-Ac-DON to DON, while the other 17 samples required 173–734 min. From these results it followed that for eight of the 25 individuals, conversion of 15-Ac-DON to DON was estimated to be incomplete during the 1848 min intestinal transit time. The results thus indicate substantial interindividual as well as compound specific differences in the deconjugation of acetylated DONs. A spearman correlation analysis showed a statistically significant relationship between deconjugation of both acetyl-DONs at 4 h and 24 h incubation. Based on the in vitro kinetic parameters and their scaling to the in vivo situation, it was concluded that for a substantial number of human individuals the deconjugation of 15-Ac-DON may not be complete upon intestinal transit.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Estimation Of Microbial Transformation Of 3-ac-don and 15-ac...mentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Interindividual Differences in In Vitro Human Intestinal Microbial Conversion of 3-Acetyl-DON and 15-Acetyl-DON

Jin

Rietjens

et al. 2022

Toxins

Self Cite

View full text Add to dashboard Cite

show abstract

Recent advances on formation, transformation, occurrence, and analytical strategy of modified mycotoxins in cereals and their products

et al. 2023

View full text Add to dashboard Cite

Comparative Microbial Conversion of Deoxynivalenol and Acetylated Deoxynivalenol in Different Parts of the Chicken Intestine as Detected In Vitro and Translated to the In Vivo Situation

Jin

Fall

Liu

et al. 2021

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

To assess potential differences in the intestinal microbial metabolism of deoxynivalenol (DON) and its acetylated forms 3-Ac-DON and 15-Ac-DON, in vitro anaerobic incubations with intestinal contents from chickens were conducted. Quantitative microbiota characterization was obtained by 16S rRNA sequencing. The data showed substantial differences in the level of different toxin conversions by the microbiota from the different intestinal segments. The transformation rate of DON to its metabolite DOM-1 decreased in the order of cecum > ileum > jejunum, and caecum contents could completely transform DON to DOM-1 within 24 h. However, no transformation appeared in the duodenum. For 3-Ac-DON, the deacetylation rate decreased as follows: duodenum > caecum > ileum > jejunum, and 100% deacetylation was observed in the duodenum within 24 h. The deacetylation of 15-Ac-DON decreased in the order of cecum > duodenum > ileum with no transformation in the jejunum. Some genera may contribute to the transformation of the toxins. Based on the in vitro kinetic parameters and their scaling to the in vivo situation, it was concluded that in the chicken small intestine, the deconjugation of both 3-Ac-DON and 15-Ac-DON will not likely be completed upon full transit. Whether this also holds for humans remains to be established.

show abstract

Species Differences in in vitro and Estimated in vivo Kinetics for Intestinal Microbiota Mediated Metabolism of Acetyl‐deoxynivalenols

Cited by 5 publications

References 26 publications

Interindividual Differences in In Vitro Human Intestinal Microbial Conversion of 3-Acetyl-DON and 15-Acetyl-DON

Interindividual Differences in In Vitro Human Intestinal Microbial Conversion of 3-Acetyl-DON and 15-Acetyl-DON

Recent advances on formation, transformation, occurrence, and analytical strategy of modified mycotoxins in cereals and their products

Comparative Microbial Conversion of Deoxynivalenol and Acetylated Deoxynivalenol in Different Parts of the Chicken Intestine as Detected In Vitro and Translated to the In Vivo Situation

Contact Info

Product

Resources

About