Speciation of Al in human serum by convective-interaction media fast-monolithic chromatography with inductively coupled plasma mass spectrometric detection

Murko, Simona; Milačić, Radmila; Ščančar, Janez

doi:10.1016/j.jinorgbio.2007.06.013

Cited by 32 publications

(38 citation statements)

References 32 publications

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“…Aluminum accumulates in the brain and bones of renal patients on dialysis and has been related to neurodegenerative processes [41]. In an attempt to understand aluminum intoxication, a fast protein LC method was developed to identify the aluminum carrier and/or storage in the human blood.…”

Section: Trace Compound Analysismentioning

confidence: 99%

“…In an attempt to understand aluminum intoxication, a fast protein LC method was developed to identify the aluminum carrier and/or storage in the human blood. Isolation of serum aluminum bound to high molecular weight proteins is achieved using a size-exclusion column on a fast protein LC system and applying the protein portion to a weak anion-exchange convective-interaction media (CIM) diethylamine (DEAE) fast-monolithic disk with an analytical run time of 10 min [41]. It was found that $90% of the aluminum is bound to proteins, of which 97% is tranferrin [41].…”

Section: Trace Compound Analysismentioning

confidence: 99%

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Applications of monolithic columns in liquid chromatography‐based clinical chemistry assays

Bunch

Wang

2011

J of Separation Science

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Monolithic columns have slowly been applied to HPLC methods for clinical chemistry testing in the last 10 years. The application areas include therapeutic drug monitoring, drugs of abuse, vitamins, porphyrins, and steroids. In comparison with conventional particulate columns, the monolithic columns may offer shorter chromatography time, more robustness, and better resolution for certain analytes. The potential drawback of large mobile phase consumption may be improved with smaller id columns, which are currently on the market. Methods covered in this review are those searchable in PubMed up to December 2010. This review highlights the emergence of monolithic column technology in HPLC methods used for clinical chemistry testing. The goals of this review are threefold: (i) To identify the areas of clinical chemistry that analytical monolithic columns have been used in HPLC methods. (ii) To demonstrate the application of analytical monolithic columns in HPLC methods using different detection systems. (iii) To discuss the advantages and limitations of the monolithic columns compared with particulate columns in the clinical chemistry applications.

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Section: Trace Compound Analysismentioning

confidence: 99%

Section: Trace Compound Analysismentioning

confidence: 99%

Applications of monolithic columns in liquid chromatography‐based clinical chemistry assays

Bunch

Wang

2011

J of Separation Science

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“…Murko et al [25] developed a new analytical procedure using a CIM DEAE disk with ICP -MS detection for the speciation of high molecular mass (HMM) Al species in spiked human serum. Special problems in speciation of Al in biological samples are low serum Al concentrations and contamination by extraneous Al.…”

Section: Speciation Of Al In Human Serummentioning

confidence: 99%

“…In addition, special attention should be devoted to cleaning the disk support. The robustness and chemical stability of methacrylate-based monoliths [27] allowed rigorous cleaning with 1 mol/L NaOH and 2 mol/L citric acid [25], which efficiently remove Al from the chromatographic support. Considering also the efficient cleaning of reagents used in the chromatographic procedure the overall blank was lowered to below 0.5 ng Al/mL, and was similar to that obtained for speciation of Al in spiked human serum by applying anion-exchange FPLC -ETAAS [29].…”

Section: Speciation Of Al In Human Serummentioning

confidence: 99%

“…Cation-and anion-exchange methacrylate-based monolithic supports also represent potential for fast separation of charged chemical species of elements. Complementary to anion-and cation-exchange fast protein liquid chromatography (FPLC), CIM monolithic disks were used for speciation of zinc (Zn) in environmental [23], chromium (Cr) in occupational health [24], and aluminium (Al) [25] in serum samples. In these studies the possibilities of the use of methacrylate-based monolithic chromatography were investigated, regarding critical parameters in speciation analysis, such as selectivity, sensitivity, and time of analysis.…”

Section: Introductionmentioning

confidence: 99%

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Applications of methacrylate‐based monolithic supports for speciation analysis

Ščančar

Milačić

2009

J of Separation Science

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Liquid chromatography combined with element specific detection is commonly applied in speciation analysis. In these analyses, to obtain reliable data chemical species should not be transformed. To preserve chemical species during the separation step, fast chromatographic procedures and mild separation conditions are required. Monolithic supports that enable rapid chromatographic separations have rarely been used in speciation analysis. Methacrylate-based anion- and cation-exchange monolithic supports offer separation of charged chemical species of elements and can be used as a complementary tool to particle-packed liquid chromatographic columns. The present paper presents an overview of successful applications of methacrylate-based monolithic supports in speciation of zinc (Zn), chromium (Cr), and aluminium (Al) in environmental, occupational health, and biological samples. Measures of analytical performance of convective interaction media (CIM) monolithic chromatographic supports, namely selectivity, sensitivity, and time of analysis, are compared to those of particle-packed columns. The potential of CIM monolithic chromatography in speciation analysis is critically discussed. Direct comparison of the experimental data in speciation of elements by ion-exchange monolithic and fast protein liquid chromatography is reported for the first time. Finally, some recommendations are given for further investigations of the potential of monolithic chromatography and its implementations in different fields of element speciation analysis.

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Analytical Procedures for Speciation of Chromium, Aluminum, and Tin in Environmental and Biological Samples

Michalke

2016

Metallomics

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Speciation of Al in human serum by convective-interaction media fast-monolithic chromatography with inductively coupled plasma mass spectrometric detection

Cited by 32 publications

References 32 publications

Applications of monolithic columns in liquid chromatography‐based clinical chemistry assays

Applications of monolithic columns in liquid chromatography‐based clinical chemistry assays

Applications of methacrylate‐based monolithic supports for speciation analysis

Analytical Procedures for Speciation of Chromium, Aluminum, and Tin in Environmental and Biological Samples

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