Special Problems with the Extraction of Plants

Silva, Gloria L.; Lee, Ik‐Soo; Kinghorn, A. Douglas

doi:10.1007/978-1-59259-256-2_12

Cited by 131 publications

(133 citation statements)

References 32 publications

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“…The ethyl acetate extract was screened for the presence of secondary metabolites using standard methods, (Silva et al, 1998).…”

Section: Preliminary Phytochemical Screening Of the Extractmentioning

confidence: 99%

Evaluataion of the Phytochemical and Antimicrobial Properties of Ethyl acetate Leaf Extract of Palicourea croceiodes

2017

International Journal of Advanced Research in Chemical Science

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“…The ethyl acetate extract was screened for the presence of secondary metabolites using standard methods, (Silva et al, 1998).…”

Section: Preliminary Phytochemical Screening Of the Extractmentioning

confidence: 99%

Evaluataion of the Phytochemical and Antimicrobial Properties of Ethyl acetate Leaf Extract of Palicourea croceiodes

2017

International Journal of Advanced Research in Chemical Science

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“…The yield of powder was 48% from water extracts, 34% from acetone and 21% from ethanol extracts for the root while 51%, 32% and 19% (w/w) were obtained for the stem bark, respectively. Farnsworth (1966), Eaton (1989), Silva et al (1993), Harborne (1998) and Houghton and Raman (1998).…”

Section: Preparation Of Extractsmentioning

confidence: 99%

“…Steroidal and triterpenes saponins were distinguished using the Liebermann-Burchard test (Farnsworth, 1966). The presence of flavonoids was determined using magnesium powder and a few drops of HCl test (Silva et al, 1993).…”

Section: Source Of Test Organismsmentioning

confidence: 99%

Antimicrobial Potency of Hydro, acetone and Ethanolic extracts of <i>Treculia africana</i> Decne Root and Stem Bark

Oyeyipo¹,

Onasoga²

2016

Journal of Applied Sciences and Environmental Management

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ABSTRACT:Treculia africana is a plant that is used in traditional medicine for the treatment of diverse ailments. To investigate the scientific basis for the use of the plant, the antimicrobial activities/potency of the root and stem bark extracts were evaluated against common gram negative and gram positive bacteria as well as yeast. The chemical constituents of the plant in addition to the effect of temperature and pH on its antimicrobial potencies were investigated. The phytochemical constituents of the dried powdered plant parts were extracted using aqueous and organic solvents (acetone and ethanol). The antimicrobial activity of the concentrated extracts was evaluated by determining the diameter of zone of inhibition against both gram negative and gram positive bacteria and yeast, using the disc diffusion technique. Phytochemical studies revealed the presence of glycosides, anthraquinone, free aglycone, saponin, steroids and polyphenols. The extracts were active against both gram positive and gram negative bacteria. The activities of the plant extracts were not affected at varied temperatures (4 o C, 30 o C, 60 o C and 100 o C), but decreased at alkaline pH. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts on the test organisms revealed lowest MIC and MBC against Salmonella paratyphi, Salmonella typhi and Bacillus subtilis with highest MIC and MBC exhibited against Staphylococcus aureus. Generally, there was significant difference (p<0.05) in the antimicrobial activities of the test extracts and control antibiotics, with the control antibiotics exerting broader antimicrobial activity. Treculia africana demonstrated broad spectrum antibacterial activity and a potential source of new classes of antibiotics that could be useful for infectious disease chemotherapy and control. ©JASEM

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“…Pelarut yang digunakan diganti setiap 24 jam sekali (Silva et al, 1998). Proses maserasi dilakukan secara berulangulang sampai diperoleh larutan jernih.…”

Section: Pembuatan Ekstrak Daun Sernaiunclassified

“…Larutan hasil maserasi disaring dengan menggunakan kapas dan kertas saring. Kemudian ekstrak diuapkan dengan menggunakan rotary evaporator pada suhu berkisar 30-40 C (Silva et al, 1998) sampai diperoleh ekstrak kasar/kental.…”

Section: Pembuatan Ekstrak Daun Sernaiunclassified

UJI AKTIVITAS EKSTRAK DAUN SERNAI (Wedelia biflora) sebagai ANTITRIPANOSOMA PADA TIKUS PUTIH (Rattus norvegicus)

Eliawardani¹

2015

J.Med.Vet.

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ABSTRAKPenelitian ini bertujuan mengetahui penurunan persentase parasitemia darah tikus yang diinfeksi Trypanosoma evansi (T. evansi) dan diberi ekstrak daun sernai (Wedelia biflora). Penelitian ini menggunakan rancangan acak lengkap (RAL). Dua puluh ekor tikus jantan dibagi secara acak ke dalam 4 kelompok perlakuan yang masing-masing terdiri atas 5 ekor tikus. Kelompok K1 (kontrol positif) adalah tikus yang diinfeksikan dengan 10 3 T. evansi dan tidak diberikan ekstrak daun sernai, kelompok K2, K3, dan K4 adalah tikus yang diinfeksikan dengan 10 3 T. evansi dan diberikan ekstrak daun sernai secara oral dengan dosis masing-masing 30, 45, dan 60 mg/kg bobot badan selama 3 hari berturut-turut. Infeksi T. evansi dilakukan secara intra peritoneum sedangkan ekstrak diberikan secara oral selama 3 hari berturut-turut. Hasil penelitian menunjukkan bahwa rata-rata ± SD persentase parasitemia tikus dari K2, K3, dan K4 lebih rendah dari K1. Dari penelitian ini dapat disimpulkan bahwa infeksi T. evansi meningkatkan persentase parasitemia dan pemberian ekstrak daun sernai berbagai dosis mampu menurunkan persentase parasitemia pada tikus. ____________________________________________________________________________________________________________________ Kata kunci: parasitemia, Trypanosoma evansi, Wedelia biflora ABSTRACT This research was aimed at investigating decrease percentage of parasitemia as an indicator of anti trypanosomal activity of sernai leaves (Wedelia biflora) extracts on rat (Rattus novergicus) infected with Trypanosoma evansi. This research was using completely randomized design. Subjects were 20 male rats randomly assigned to 4 groups consisting of 5 rats. Group K1 (positive control) were rats infected with 10 3 T. evansi, group K2, K3 and K4 were rats infected with 10 3 T. evansi and treated with 30, 45 and 60 mg/kg body of sernai leaves extract. Infection was performed by intra peritoneal injection and sernai leaves extract was given orally for 3 consecutive days. Result of this research showed that percentage of parasitemia from KII, KIII, and KIV rats were lower than those of control rats (KI). It can be concluded that infection of T. evansi increase percentage of parasitemia of rats and administration various doses of sernai leaves extract decrease percentage of parasitemia in rats.____________________________________________________________________________________________________________________

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Special Problems with the Extraction of Plants

Cited by 131 publications

References 32 publications

Evaluataion of the Phytochemical and Antimicrobial Properties of Ethyl acetate Leaf Extract of Palicourea croceiodes

Evaluataion of the Phytochemical and Antimicrobial Properties of Ethyl acetate Leaf Extract of Palicourea croceiodes

Antimicrobial Potency of Hydro, acetone and Ethanolic extracts of <i>Treculia africana</i> Decne Root and Stem Bark

UJI AKTIVITAS EKSTRAK DAUN SERNAI (Wedelia biflora) sebagai ANTITRIPANOSOMA PADA TIKUS PUTIH (Rattus norvegicus)

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