Spatial Recombinant Human Bone Morphogenetic Protein 2 Delivery from Hydroxyapatite Scaffolds Sustains Bone Regeneration in Rabbit Radius

Cell-Instructive Mineralized Microenvironment Regulates Osteogenesis: A Growing SYMBIOSIS of Cell Biology and Biomaterials Engineering in Bone Tissue Regeneration

“…The scaffold designed to treat bone defects should mimic the native bone microenvironment to attract native stem cells at the defect site and instruct them to differentiate into osteoblasts. HAp, a significant component of native bone, is well known for its osteoconductive and osteoinductive abilities. , There are different ways to create HAp-rich three-dimensional (3D) microenvironments to improve osteogenesis, both in vitro and in vivo . − A recent paper revealed that the form in which HAp is applied differentially regulates cell morphology and cell binding receptors . Akin to in vivo situations, a 3D milieu promotes higher interactions between the cells and the osteoinductive molecules and increases the chances of early osteogenesis .…”

Preparation and characterization of 3D hydroxyapatite/collagen scaffolds and its application in bone regeneration with bone morphogenetic protein-2

“…[18][19][20] Even though there are currently some relevant studies on HA/Col scaffolds impregnated with recombinant human bone morphogenetic proteins 2 (rhBMP-2) or spatially-controlled delivery of rhBMP-2 from HA scaffolds coated with collagen I lm for bone regeneration, either of them lacking the chemical characterization and exploration between them, or the comprehensive and cell experiments in vitro. 21,22 In the present work, we assembled a 3D-type composite scaffold from different ratios of Col and nano-HA by optimized blending and freeze-drying methods, then the properties of HA/ Col were characterized and assessed for choosing the most suitable materials for bioremediation. To further improve their ability to induce bone formation and regeneration, the HA/Col was further with the rhBMP-2 with slow release during application.…”

“…Even though there are currently some relevant studies on HA/Col scaffolds impregnated with recombinant human bone morphogenetic proteins 2 (rhBMP-2) or spatially-controlled delivery of rhBMP-2 from HA scaffolds coated with collagen I film for bone regeneration, either of them lacking the chemical characterization and exploration between them, or the comprehensive and cell experiments in vitro . 21,22…”