Preparation and characterization of 3D hydroxyapatite/collagen scaffolds and its application in bone regeneration with bone morphogenetic protein-2

Xie, Haiyang; Ruan, Sijie; Zhao, Ming; Long, Jindong; Ma, Xiao‐Jun; Guo, Jinhong; Lin, Xiaofei

doi:10.1039/d3ra03034b

Cited by 7 publications

(2 citation statements)

References 40 publications

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“…Since biocomposites of CaPO 4 with collagen were found to be able to form bioconjugates (bioconjugation is a chemical strategy to form a stable covalent link between two molecules, at least one of which is a biomolecule [784]), they are beneficial for the delivery of drugs, growth factors, and other biomolecules [610,720,765,[785][786][787]. Namely, 5 weeks after implantation, HA/collagen-alginate (20 µL) supplemented with rhBMP-2 (100 µg/mL, 15 µL) demonstrated osteogenesis along the implant without the material deforming much [610].…”

Section: Collagen-containing Biocompositesmentioning

confidence: 99%

Calcium Orthophosphate (CaPO4) Containing Composites for Biomedical Applications: Formulations, Properties, and Applications

Dorozhkin

2024

J. Compos. Sci.

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The goal of this review is to present a wide range of hybrid formulations and composites containing calcium orthophosphates (abbreviated as CaPO4) that are suitable for use in biomedical applications and currently on the market. The bioactive, biocompatible, and osteoconductive properties of various CaPO4-based formulations make them valuable in the rapidly developing field of biomedical research, both in vitro and in vivo. Due to the brittleness of CaPO4, it is essential to combine the desired osteologic properties of ceramic CaPO4 with those of other compounds to create novel, multifunctional bone graft biomaterials. Consequently, this analysis offers a thorough overview of the hybrid formulations and CaPO4-based composites that are currently known. To do this, a comprehensive search of the literature on the subject was carried out in all significant databases to extract pertinent papers. There have been many formulations found with different material compositions, production methods, structural and bioactive features, and in vitro and in vivo properties. When these formulations contain additional biofunctional ingredients, such as drugs, proteins, enzymes, or antibacterial agents, they offer improved biomedical applications. Moreover, a lot of these formulations allow cell loading and promote the development of smart formulations based on CaPO4. This evaluation also discusses basic problems and scientific difficulties that call for more investigation and advancements. It also indicates perspectives for the future.

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Section: Collagen-containing Biocompositesmentioning

confidence: 99%

Calcium Orthophosphate (CaPO4) Containing Composites for Biomedical Applications: Formulations, Properties, and Applications

Dorozhkin

2024

J. Compos. Sci.

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“…Notably, both groups displayed consistent scaffolds and stable cell growth as the culture time increased. 18,19 The core of bone regeneration applications lies in the effect of the sustained-release anti-tuberculosis drug PaMZ bioscaffold on the osteogenic differentiation ability of rBMSCs. Alkaline phosphatase (ALP), an essential component of hydrolyzed organophosphorus in bone matrix vesicles, plays a crucial role in the initial stage of bone matrix mineralization and serves as an early indicator of the osteogenic phenotype.…”

Section: B In Vitro Osteogenic Studies Of Drug-loaded Artificial Scaf...mentioning

confidence: 99%

Preparation and in vitro osteogenic evaluation of high-strength ceramic artificial bone loaded with anti-tuberculosis drug PaMZ

Li,

Fei,

Zhang

et al. 2023

AIP Advances

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The objective of this study was to prepare a high-strength ceramic artificial bone loaded with the anti-tuberculosis drug PaMZ (delamanid, moxifloxacin, and pyrazinamide) and evaluate its physical characteristics and osteogenic potential. We utilized 3D printing technology to fabricate artificial bones and then obtained a high-strength ceramic artificial bone by high-temperature firing. Then, a triple combination of anti-tuberculosis drugs, including delamanid (Pa), moxifloxacin (M), pyrazinamide (Z), and polylactic acid-co-glycolic acid mixed in a ratio of 3:12:45:140, was incorporated onto the surface of the ceramic artificial bone. Consequently, a high-strength ceramic artificial bone, loaded with anti-tuberculosis drugs, was successfully obtained. The physical characteristics of the drug-loaded artificial bone were assessed using an electronic universal testing machine and scanning electron microscopy. The osteogenic performance of the artificial bone was evaluated through rat bone marrow mesenchymal stem cell (rBMSCs) co-culture experiment, cell counting kit-8 (CCK-8) cell proliferation assay, alkaline phosphatase staining, and alizarin red staining. The drug-loaded ceramic artificial bone exhibited favorable physical characteristics, void interconnection, a porosity of 30.6% ± 0.7%, and a compressive strength of 17.65 ± 0.46 MPa. The rBMSCs co-culture experiment and CCK-8 cell proliferation experiment demonstrated excellent cell compatibility, while alkaline phosphatase and alizarin red staining indicated good in vitro osteogenic performance. In summary, the high-strength ceramic artificial bone loaded with the anti-tuberculosis drug PaMZ exhibited a favorable morphological structure and compressive strength. In addition, it demonstrated good biocompatibility and osteogenic properties.

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Applications of Natural Product-Based Scaffold in Tissue Engineering

Kaur

2024

Natural Product Inspired Scaffolds

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Preparation and characterization of 3D hydroxyapatite/collagen scaffolds and its application in bone regeneration with bone morphogenetic protein-2

Abstract: Desirable bone engineering materials should have a conducive three-dimensional (3D) structure and bioactive mediators for guided bone regeneration.

Cited by 7 publications

References 40 publications

Calcium Orthophosphate (CaPO4) Containing Composites for Biomedical Applications: Formulations, Properties, and Applications

Calcium Orthophosphate (CaPO4) Containing Composites for Biomedical Applications: Formulations, Properties, and Applications

Preparation and in vitro osteogenic evaluation of high-strength ceramic artificial bone loaded with anti-tuberculosis drug PaMZ

Applications of Natural Product-Based Scaffold in Tissue Engineering

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