Soybean Proteomics for Unraveling Abiotic Stress Response Mechanism

Abstract

Plant response to abiotic stresses depends upon the fast activation of molecular cascades involving stress perception, signal transduction, changes in gene and protein expression and post-translational modification of stress-induced proteins. Legumes are extremely sensitive to flooding, drought, salinity and heavy metal stresses, and soybean is not an exception of that. Invention of immobilized pH gradient strips followed by advancement in mass spectrometry has made proteomics a fast, sensitive and reliable technique for separation, identification and characterization of stress-induced proteins. As the functional translated portion of the genome plays an essential role in plant stress response, proteomic studies provide us a finer picture of protein networks and metabolic pathways primarily involved in stress tolerance mechanism. Identifying master regulator proteins that play key roles in the abiotic stress response pathway is fundamental in providing opportunities for developing genetically engineered stress-tolerant crop plants. This review highlights recent contributions in the field of soybean biology to comprehend the complex mechanism of abiotic stress acclimation. Furthermore, strengths and weaknesses of different proteomic methodologies of extracting complete proteome and challenges and future prospects of soybean proteome study both at organ and whole plant levels are discussed in detail to get new insights into the plant abiotic stress response mechanism.

Gel-free/label-free proteomic analysis of root tip of soybean over time under flooding and drought stresses

“…Comparisons of the protein abundance of these four organs indicated that the root tip was the most responsive organ to flooding, displaying the largest changes of protein abundance. Under drought stress, the root was the most sensitive organ, a finding that was also reported by Hossain et al [41]. The different sensitive organs under flooding and drought imply different mechanisms might be involved for soybeans to struggle against stresses.…”

“…Soybean is sensitive to many abiotic stresses, including cold, salinity, flooding, and drought [41]. However, flooding and drought have particularly adverse effects on soybean growth, especially at the seedling stage [17,23,24].…”

Functional and Integrative Analysis of the Proteomic Profile of Radish Root under Pb Exposure

“…Additionally, many thiol-containing antioxidants, peroxiredoxin (PRX33, unigene27310), glutaredoxin-like protein (GRX, CL10604.Contig2) and thioredoxin (TRX, CL13300.Contig1), were also found to be altered during the Pb-stressed condition of radish root. The Prx is a thiol peroxidase with multiple functions, which was found to be induced under various HM stresses such as Cd (Hossain et al, 2013) and As (Pandey et al, 2012). Glutaredoxin (GRX) and thioredoxin (TRX) could be oxidized by peroxides and regenerate peroxiredoxins, which were verified to play direct roles in the antioxidative system by regenerating peroxiredoxins oxidized by peroxides (Hossain and Komatsu, 2013).…”

Role of Proteomics in Crop Stress Tolerance

“…The accumulation of dehydrin and ferritin were identified in proteomic investigation of soybean roots under drought stress (Alam et al, 2010a,b; Nouri et al, 2011). Dehydrins are LEA proteins, can effectively improved plant growth under stress by reducing the harmful effect of ROS (Grelet et al, 2005; Nouri et al, 2011; Hossain et al, 2013). Grelet et al (2005) identified a LEA protein, PsLEAm localized within the matrix space of seed mitochondria of Pisum sativum .…”