Soy isoflavone genistein inhibits hsa_circ_0031250/miR‐873‐5p/FOXM1 axis to suppress non‐small‐cell lung cancer progression

Abstract

The foods of plants provide the rich nutrition and have protective function in human diseases, including cancers. Genistein is a major isoflavone constituent in soybeans, which has an anti-cancer role in non-small-cell lung cancer (NSCLC). Nevertheless, the mechanism underlying the anti-cancer function of genistein in NSCLC remains largely unknown. NSCLC cells (H292 and A549) were exposed to genistein. Circular RNA hsa_circ_0031250 (circ_0031250), microRNA (miR)-873-5p and forkhead box M1 (FOXM1) abundances were examined via quantitative reverse transcription polymerase chain reaction and Western blotting. The function of genistein, circ_0031250, miR-873-5p, and FOXM1 on NSCLC progression was investigated via Cell Counting Kit-8, colony formation, transwell well, wound healing, flow cytometry, Western blotting and xenograft model. The target relationship was analyzed by dualluciferase reporter analysis and RNA immunoprecipitation. Results showed that genistein inhibited NSCLC cell viability in dose-time-dependent patterns. circ_0031250 abundance was elevated in NSCLC samples and cell lines, and it was reduced via genistein exposure. circ_0031250 knockdown aggravated genistein-caused suppression of cell proliferation, migration and invasion and elevation of apoptosis. miR-873-5p expression was decreased in NSCLC samples and cells. miR-873-5p was targeted via circ_0031250, and miR-873-5p knockdown attenuated the influence of circ_0031250 silence on NSCLC progression in the presence of genistein. FOXM1 was regulated via circ_0031250/ miR-873-5p axis. miR-873-5p constrained cell proliferation, migration and

Long noncodingRNA MCF2L‐AS1promotes the cancer stem cell‐like traits in non‐small cell lung cancer cells through regulatingmiR‐873‐5p level

“…Notably, miR-873-5p has been shown to be a critical suppressor for several cancer types, such as colon cancer, 15 cervical cancer, 16 papillary thyroid cancer, 17 and NSCLC. 18 These results strongly hint that miR-873-5p is involved in MCF2L-AS1-mediated effects on the CSC-like traits of NSCLC cells. Indeed, our results showed that miR-873-5p negatively regulates the CSC-like traits of NSCLC cells.…”

“…RNA‐pull down combined with luciferase reporter analysis confirmed that lncRNA MCF2L‐AS1 indeed can bind to miR‐873‐5p. Notably, miR‐873‐5p has been shown to be a critical suppressor for several cancer types, such as colon cancer, 15 cervical cancer, 16 papillary thyroid cancer, 17 and NSCLC 18 . These results strongly hint that miR‐873‐5p is involved in MCF2L‐AS1‐mediated effects on the CSC‐like traits of NSCLC cells.…”

Soybean Seed Compounds as Natural Health Protectors

“…It is known that nutrition is very important in lung health and defense [46][47][48]. Protection against lung cancer and the radioprotective effect of soy isoflavones are well described in the bibliography [49,50]. Soy isoflavones seem to increase chemotherapy induced cell death in different types of lung cancer [51,52].…”

MiR-873-5p: A Potential Molecular Marker for Cancer Diagnosis and Prognosis

“…In addition, genistein is a soy-derived isoflavone that can play a beneficial role in cancer treatment ( 130 ). Genistein can inhibit the progression of NSCLC by regulating the circ_0031250/miR-873-5p/FOXM1 axis ( 131 ).…”

“…Knockout of circ_0004507 can up-regulate the expression of miR-873-5p and inhibit the progression of laryngeal cancer ( 137 ). circ_0031250 can promote the proliferation, migration, and invasion of NSCLC cells by inhibiting the miR-873-5p/FOXM1 axis ( 131 ). circZKSCAN1 can inhibit the progression, proliferation, migration, and invasion of HCC by down-regulating the miR-873-5p/DLC1 axis, thereby hindering the occurrence and development of HCC ( 138 ).…”