Sorting algal cells by morphology in spiral microchannels using inertial microfluidics

Schaap, Allison; Dumon, Jéromine; Toonder, Jaap M.J. den

doi:10.1007/s10404-016-1787-1

Cited by 42 publications

(36 citation statements)

References 41 publications

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“…With regard to shape-based separation of candida cells using our device, it was confirmed that separation efficiency is affected by asymmetry due to the shape of the formed germ tube. In other studies, the device enables the separation of cells based on shape differences without curvature, which can be a useful biomarker indicating cell type, cell cycle, and differences based on environmental conditions (e.g., fungal pathogens, microalgae, and rod-shaped bacteria (e.g., bacilli)) [34,46,47,68]. In addition, additional numerical analyses and simulations are required to calculate the elastic force exerted on nonspherical particles/cells and to predict the flow characteristics of those in the viscoelastic fluid.…”

Section: Resultsmentioning

confidence: 99%

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Sheathless Shape-Based Separation of Candida Albicans Using a Viscoelastic Non-Newtonian Fluid

et al. 2019

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Rapid and accurate identification of Candida albicans from among other candida species is critical for cost-effective treatment and antifungal drug assays. Shape is a critical biomarker indicating cell type, cell cycle, and environmental conditions; however, most microfluidic techniques have been focused only on size-based particle/cell manipulation. This study demonstrates a sheathless shape-based separation of particles/cells using a viscoelastic non-Newtonian fluid. The size of C. albicans was measured at 37 °C depending on the incubation time (0 h, 1 h, and 2 h). The effects of flow rates on the flow patterns of candida cells with different shapes were examined. Finally, 2-h-incubated candida cells with germ tube formations (≥26 μm) were separated from spherical candida cells and shorter candida cells with a separation efficiency of 80.9% and a purity of 91.2% at 50 μL/min.

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Section: Resultsmentioning

confidence: 99%

“…In addition, a viscoelastic sheathless shape-based separation technique has been applied to drug-treated human fungal pathogens using differences in morphology [46]. However, there is still room for improvement in device throughput and applications to various biological particles with different shapes [34,47].…”

Section: Introductionmentioning

confidence: 99%

Sheathless Shape-Based Separation of Candida Albicans Using a Viscoelastic Non-Newtonian Fluid

et al. 2019

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“…Son et al employed inertial separation in the curved microchannel to purify sperm cells from RBCs and leukocytes [300,301]. Schaap et al first demonstrated the microfluidic separation of three specimens of algal cells of various sizes and shapes, including non-spherical ones [302]. Lee et al used inertial separation in the curved channel for mesenchymal stem cell enrichment from a tissue-digested mouse bone marrow cell mixture [303].…”

Section: Inertial Focusing In Microfluidic Channelsmentioning

confidence: 99%

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Voronin

Kozlova

Verkhovskii

et al. 2020

IJMS

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Flow cytometry nowadays is among the main working instruments in modern biology paving the way for clinics to provide early, quick, and reliable diagnostics of many blood-related diseases. The major problem for clinical applications is the detection of rare pathogenic objects in patient blood. These objects can be circulating tumor cells, very rare during the early stages of cancer development, various microorganisms and parasites in the blood during acute blood infections. All of these rare diagnostic objects can be detected and identified very rapidly to save a patient’s life. This review outlines the main techniques of visualization of rare objects in the blood flow, methods for extraction of such objects from the blood flow for further investigations and new approaches to identify the objects automatically with the modern deep learning methods.

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“…They can simulate small-scale fluid flow and chemical gradients and offer full manual control over the particles to study the desired details, e.g., for food market, clinical, pharmaceutical, and other applications [1][2][3][4][5][6][7]. Precise manipulations such as focusing, separation, and fractionation of cells is a vital capability of microfluidics which can be achieved by engineering hydrodynamics forces based on unique physical attributes of cells such as size [8,9], density [9,10], deformability [11][12][13], and morphology [14] using variety of methods such as crossflow filtration [15], electrode arrays [16], optical force switching [17] and other methods, some of and other methods, some of which can be found in detail in the review presented in [18]. In this spirit, hydrodynamic phenomena, which carry microenvironmental physical impacts on cells, are critical in almost all physiological functions and bodily systems.…”

Section: Microfluidic Systems For Cellular Flow Manipulationmentioning

confidence: 99%

Advances in Computational Fluid Mechanics in Cellular Flow Manipulation: A Review

2019

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Recently, remarkable developments have taken place, leading to significant improvements in microfluidic methods to capture subtle biological effects down to single cells. As microfluidic devices are getting sophisticated, design optimization through experimentations is becoming more challenging. As a result, numerical simulations have contributed to this trend by offering a better understanding of cellular microenvironments hydrodynamics and optimizing the functionality of the current/emerging designs. The need for new marketable designs with advantageous hydrodynamics invokes easier access to efficient as well as time-conservative numerical simulations to provide screening over cellular microenvironments, and to emulate physiological conditions with high accuracy. Therefore, an excerpt overview on how each numerical methodology and associated handling software works, and how they differ in handling underlying hydrodynamic of lab-on-chip microfluidic is crucial. These numerical means rely on molecular and continuum levels of numerical simulations. The current review aims to serve as a guideline for researchers in this area by presenting a comprehensive characterization of various relevant simulation techniques.

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Sorting algal cells by morphology in spiral microchannels using inertial microfluidics

Abstract: simplicity of inertial microfluidics make it appropriate for the high-throughput pre-sorting of algae cells upstream of other integrated sensing modalities in a field-deployable device.

Cited by 42 publications

References 41 publications

Sheathless Shape-Based Separation of Candida Albicans Using a Viscoelastic Non-Newtonian Fluid

Sheathless Shape-Based Separation of Candida Albicans Using a Viscoelastic Non-Newtonian Fluid

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Advances in Computational Fluid Mechanics in Cellular Flow Manipulation: A Review

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