Soroprevalência de Borrelia burgdorferi latu sensu associada à presença de carrapatos em cães de áreas rurais do Estado do Rio de Janeiro, Brasil

O’Dwyer, Lúcia Helena; Soares, Cleber Oliveira; Massard, Carlos Luiz; Souza, José Carlos Pereira de; Flausino, Walter; Fonseca, Adivaldo Henrique da

doi:10.1590/s0103-84782004000100031

Cited by 9 publications

(8 citation statements)

References 19 publications

(13 reference statements)

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“…However, since 80% dogs' sera also demonstrated antibodies against p100 by WB (Table 1), an antigen for which no cross-reactivity has been reported, the antibodies detected here are likely a result of Borrelia infection. In addition, the present study corroborates previous studies (O'DWYER et al, 2004) in which no association was established between seropositivity to Borrelia spp. and age, gender, or presence of ticks in dogs.…”

Section: Discussionsupporting

confidence: 92%

“…Antibody seropositivity to Borrelia spp. was found by WB in 18% of dogs in the present study, which is a higher prevalence than has been reported previously in tick endemic areas (JOPPERT et al, 2001;O'DWYER et al, 2004). Previous studies have shown cross-reactivity with antigens of other spirochetes, such as Leptospira antigens p41 and OspC (BRUCKBAUER et al, 1992;LESCHNIK et al, 2010), and it is important to note the possibility that dog sera in this study (untested for anti-Leptospira antibodies) may have tested positive by WB as result of cross-reactivity.…”

Section: Discussioncontrasting

confidence: 46%

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Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil

Nascimento

Vieira

et al. 2016

Rev. Bras. Parasitol. Vet.

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The aims of the present study were to serosurvey dogs, horses, and humans highly exposed to tick bites for anti-Borrelia burgdorferi s.l. antibodies, identify tick species present, and determine risk factors associated with seropositivity in a rural settlement of Paraná State, southern Brazil. Eighty-seven residents were sampled, along with their 83 dogs and 18 horses, and individual questionnaires were administered. Immunofluorescence antibody test (IFAT) was performed on serum samples and positive samples were subjected to western blot (WB) analysis. Anti-B. burgdorferi antibodies were found in 4/87 (4.6%) humans, 26/83 (31.3%) dogs, and 7/18 (38.9%) horses by IFAT, with 4/4 humans also positive by WB. Ticks identified were mostly from dogs and included 45/67 Rhipicephalus sanguineus, 21/67 Amblyomma ovale, and 1/67 A. cajennense sensu lato. All (34/34) horse ticks were identified as A. cajennense s.l.. No significant association was found when age, gender, or presence of ticks was correlated to seropositivity to Borrelia sp. In conclusion, although anti-Borrelia antibodies have been found in dogs, horses and their owners from the rural settlement, the lack of isolation, molecular characterization, absence of competent vectors and the low specificity of the commercial WB kit used herein may have impaired risk factor analysis.

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Section: Discussionsupporting

confidence: 92%

Section: Discussioncontrasting

confidence: 46%

Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil

Nascimento

Vieira

et al. 2016

Rev. Bras. Parasitol. Vet.

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“…Também não houve diferença significativa (P < 0,05) em relação à função desempenhada pelos animais. No presente estudo, não foi observada diferença significativa entre as freqüencias de animais soropositivos dos diferentes municípios e o resultado corrobora com os encontrados em outras espécies de animais domésticos no Brasil, como bovinos (Fonseca et al 1996) e caninos (O'Dwyer et al 2004). Resultados semelhantes foram reportados nos Estados Unidos da América, onde foram observadas soropositividade que variavam entre 12 e 75% em eqüinos sem sintomatologia clínica para borreliose de Lyme (Bernard et al 1990, Cohen et al 1988, Cohen et al 1992, Marcus et al 1985, Parker & White 1992.…”

Section: Resultsunclassified

“…Palácios et al (1999) No presente trabalho não houve diferença significativa entre a distribuição da prevalência de anticorpos anti-B.burgdorferi quanto a raças e sexos. Estes resultados estão de acordo com os observados por Salles et al 2002, O'dwyer et al 2004e Madureira et al 2007. Marcus et al (1985) observaram que nos EUA a freqüência de B. burgdorferi em eqüinos foi maior em áreas endêmicas que em áreas não endêmicas com soropositividade de 24% e 2%, respectivamente, não havendo diferença significativa entre raças e sexo.…”

Section: Resultsunclassified

Freqüência de anticorpos homólogos anti-Borrelia burgdorferi em eqüinos na mesorregião metropolitana de Belém, Estado do Pará

et al. 2009

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Espiroquetas transmitidas por carrapatos são microrganismos de ampla distribuição geográfica e acometem animais silvestres, domésticos e seres humanos. Procedeu-se a análise sorológica de 300 soros de eqüinos onde 58 animais eram do município Ananideua, 61 eram de Belém, 131 de Castanhal e 50 eram do município de Santa Izabel do Pará para Borrelia burgdorferi através do teste ELISA indireto. Não foram observadas diferenças significativas (P < 0,05) entre os municípios, nem quanto à raça, sexo e função dos animais. Um total de 80 (26,7%) animais foram positivos para B. burgdorferi com os títulos de 1:800, 72 (90%) eqüinos; 1:1.600, 6 (7,5%) eqüinos; e 1:3.200, 2 (2,5%) eqüinos. Os resultados observados foram similares aos descritos nos EUA, onde foram relatadas freqüências de soropositivos variando entre 7 e 75% em eqüinos assintomáticos. A presença de anticorpos homólogos contra B. burgdorferi em eqüinos na mesorregião metropolitana de Belém é indicativo da ampla distribuição do agente e da possibilidade de ocorrerem casos humanos deste agente na região.

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“…The prevalence of antiBorrelia spp. antibodies in dogs is high in Brazil, varying from 9,0% to 83.9%, according to previous studies in different regions of the country (ALVES et al, 2004;CORREIA et al, 2012;JOPPERT et al, 2001;O'DWYER et al, 2004;SOARES et al, 2000). All these studies showed a close relationship between the ticks' infestation and high antibody rates against Lyme spirochetes.…”

Section: Introductionmentioning

confidence: 76%

First record of Borrelia burgdorferi sensu lato antibodies in stray dogs in the Northwest Region of Parana State, Brazil

et al. 2015

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The aim of this study was to determine the occurrence of B. burgdorferi sensu lato s.l. antibodies in stray dogs of the urban area of Umuarama Town, localized in the northwest region of Parana state, Brazil. Serum samples from 168 dogs were tested using indirect immunofluorescence assay (IFA) and Western Blot (WB) in order to detect anti-Borrelia burgdorferi s.l. antibodies. The IFI analysis was used as screening test and positive results were confirmed employing the WB technique. Sixty-five (38.69%) of 168 serum samples were positive in the IFI; 54 of them (83.07%) were confirmed by the WB. The overall data analysis confirmed the presence of anti-B. burgdorferi s.l. antibodies in 32.14% (54/168) dogs, what suggests that northwest region of Parana State may constitute a risk area for Lyme disease. Further studies are necessary to determine the epidemiological characteristics of this disease in the region studied. Key words: Borreliosis, canids, diagnostic, IFA, western blot ResumoO objetivo deste estudo foi determinar a ocorrência de anticorpos anti-B. burgdorferi sensu lato sl em cães errantes da área urbana da cidade de Umuarama, localizado na região noroeste do estado do Paraná, Brasil. Amostras de soro de 168 cães foram testados utilizando imunofluorescência indireta (IFI) e Western Blot (WB), a fim de detectar anticorpos anti-Borrelia burgdorferi sl. A IFI foi utilizada como teste de triagem e os resultados positivos foram confirmados empregando a técnica de WB. Sessenta e cinco (38,69%) de 168 amostras de soro foram positivas na IFI; 54 deles (83,07%) foram confirmados pela WB. A análise geral dos dados confirmaram a presença de anticorpos anti-B. burgdorferi sl em

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Soroprevalência de Borrelia burgdorferi latu sensu associada à presença de carrapatos em cães de áreas rurais do Estado do Rio de Janeiro, Brasil

Abstract: RESUMO Pesquisou-se a presença de anticorpos contra

Cited by 9 publications

References 19 publications

Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil

Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil

Freqüência de anticorpos homólogos anti-Borrelia burgdorferi em eqüinos na mesorregião metropolitana de Belém, Estado do Pará

First record of Borrelia burgdorferi sensu lato antibodies in stray dogs in the Northwest Region of Parana State, Brazil

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