Some Virus Isolations From Common Colds

Biological characteristics and propagation of human rhinovirus-C in differentiated sinus epithelial cells

“…One other notable characteristic is that most HRV grow optimally at 33-34°C and have reduced replication at 37°C (Schroth et al, 1999; Taylor-Robinson & Tyrrell, 1962; Tyrrell & Parsons, 1960; Papadopoulos et al, 1999). Although the mechanism of temperature sensitivity is not completely understood, there has been a speculation that the lower GC content of HRV compared to other enteroviruses is an adaptation for replication at lower temperatures (McIntyre et al, 2010).…”

"Hand-Foot-and-Mouth Disease" in Birmingham in 1959

“…Furthermore, their viruses were isolated in tissue culture; we were unable to do this. This difference is probably explicable on technical grounds; the recent paper on the isolation of the common-cold virus by Tyrrell and Parsons (1960) illustrates the effect that a small difference in medium may make in producing a cytopathic effect. Clinically, the lesions differ from those of herpes simplex and herpangina in occurring neither on the outside surface of the lips nor on the posterior pharyngeal wall.…”

A Collaborative Study of the Aetiology of Acute Respiratory Infections in Britain 1961-4

“…Adenoviruses were recognized by their cytopathic effect and by complement-fixation tests on the culture fluid with known positive human serum; they were identified by neutralization tests with specific antisera. Human-embryo-kidney cultures were maintained in a medium containing 2 % calf serum and 0.25 % lactalbumin hydrolysate of a pH 7.4 to 6.8 and were rolled at 330 C. (Tyrrell and Parsons, 1960). Human diploid-cell strains were maintained in 2 % calf serum with Eagle's medium and were slightly more alkaline.…”