Some remarks about quantitative characterization of small particles by the electron mcroprobe

Wieser, P.; Wurster, R.

doi:10.1002/sca.4950020103

Cited by 7 publications

(3 citation statements)

References 4 publications

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“…The mechanism responsible for the large enhancement of Pb ion yield in LAMMA vs. IM measurements compared to other metals is not well understood at this time. The high Pb ion yield in LAMMA, however, is consistent with the results of Wieser et al (14). A simple local thermodynamic equilibrium (LTE) model failed to explain experimental relative elemental sensitivity factors.…”

supporting

confidence: 86%

Comparison of Laser and Ion Microprobe Detection Sensitivity for Lead in Biological Microanalysis

Linton¹,

Bryan²,

Schmidt³

et al. 1985

Anal. Chem.

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Lead detection sensitivity In doped epoxy standards was compared for the Leybold Heraeus LAMMA 500 laser microprobe vs. the Cameca IMS-3f Ion microscope (modified for high sensitivity digital Imaging). For comparable lateral spatial resolutions of about 1 µ , the LAMMA relative detection limit of 5 µ /g Is superior to that of the IMS-3f by more than 2 orders of magnitude. However, the analytical volume of the latter (0.02 µ 3) was only about 0.05 that of LAMMA measurement as the result of differences In analysis depth (300 A vs. 5000 A). Both higher Ion sputtering rates and greater analysis depths may be used to improve the relative Pb detection limit for the IMS-3f. The estimated useful Ion yield, however, remains about 2 orders of magnitude better In LAMMA (3 X 10 3 vs. 5 X 10 s). This Is consistent with experimental LAMMA studies showing enhanced Pb sensitivity compared to LTE calculations. Differences In sensitivity also are Illustrated In a comparative study of Pb distributions In atherosclerotic human aorta.

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supporting

confidence: 86%

Comparison of Laser and Ion Microprobe Detection Sensitivity for Lead in Biological Microanalysis

Linton¹,

Bryan²,

Schmidt³

et al. 1985

Anal. Chem.

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“…Polyatomic ions (fragment or recombination ions) of either the organic matrix, embedding material or supporting foil, and the major inorganic species present in the sample can interfere with the atomic mass signals elements. 5,9,50-55,85-89, 109 to be measured. For this reason the overall detection limit of the element of interest may be reduced in practical analysis.…”

Section: Detection Limits and Sensitivitymentioning

confidence: 99%

Laser microprobe mass analysis: A review of applications in the life sciences

Verbueken

Bruynseels

Grieken

1985

Biol. Mass Spectrom.

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“…Standards made by dissolving organic compounds in the resin used for sample embedding (7)(8)(9)(11)(12)(13) and thin films of vacuum deposited metals or dielectric materials (7,8) are most widespread. Another interesting possibility for LAMMA calibration could be the use of anionic surfactant films loaded with metal cations, as proposed by Gijbels et al (14).…”

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confidence: 99%

Embedded ion exchange beads as standards for laser microprobe mass analysis of biological specimens

Verbueken¹,

Grieken²,

Paulus³

et al. 1984

Anal. Chem.

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A new Internal standard system based upon Chelex-100 Ion chelating resin beads loaded with various elements Is proposed for laser microprobe mass analysis (LAMMA) of tissue-bound elements In thin sections of biological material. The beads can be coembedded with the biological specimen and cut to the desired thickness. In this way, the standard material is available together with and close to the tissue In the same section, thus avoiding section thickness measurements and specimen exchange. Chelex-100 beads can be loaded with various elements In a controlled way and the spatial elemental distribution Is sufficiently homogeneous for use In microanalysis. The signal reproducibility, the proportionality between the LAMMA response and the elemental concentration, and the Influence of the specimen composition on the LAMMA mass spectra were studied. Chelex-100 was also compared to other types of Ion exchange beads. This versatile standardization technique opens new vistas for quantitative laser microprobe applications In biological and biomedical research.Considerable interest is currently being focused on laser microprobe mass analysis (LAMMA), primarily due to the commercial advent of the LAMMA-500 instrument. A description of this new microanalytical instrument and discussions of its advantages, drawbacks, and applications can readily be found in the literature (1-3). The technique was originally developed for biomedical purposes, but hitherto its impact remained mainly limited to qualitative biological aspects.Absolute quantification of subcellular elemental distributions in biological specimens is, of course, not trivial at all. Structural localization in the uncolored and unstained histological sections is often problematic. Moreover, the LAM-MA spectra of biological preparations can be highly complex, variable, and difficult to interpret: mass interferences arising from organic molecules or fragment ions, both from the specimen itself and from the embedding or supporting media, are often important, while the laser interaction is intricate and complete understanding about laser-induced microplasma

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Some remarks about quantitative characterization of small particles by the electron mcroprobe

Cited by 7 publications

References 4 publications

Comparison of Laser and Ion Microprobe Detection Sensitivity for Lead in Biological Microanalysis

Comparison of Laser and Ion Microprobe Detection Sensitivity for Lead in Biological Microanalysis

Laser microprobe mass analysis: A review of applications in the life sciences

Embedded ion exchange beads as standards for laser microprobe mass analysis of biological specimens

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