Laser microprobe mass analysis: A review of applications in the life sciences

Verbueken, A.H.; Bruynseels, F.; Grieken, R.E. Van

doi:10.1002/bms.1200120903

Cited by 58 publications

(11 citation statements)

References 137 publications

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“…The theoretical principles of in situ analytical techniques are beyond the scope of the present review; interested readers can find more information about: EDXMA in Chandler (1979); SIMS in Linton et al (1980); LAMMA in Denoyer et al (1982) and Verbeuken, Bruynseels & Van Grieken (1985). Briefly, all these techniques rely on bombardment of the specimen with a beam of either ions (SIMS and PIXE), electrons (EDXMA) or photons (LAMMA), causing either ejection of surface atoms/ ions, which are analysed by mass spectrometry (SIMS and LAMMA), or knocking out of certain shell electrons that give rise to element-specific primary X-rays (EDXMA and PIXE).…”

Section: In Situ Techniques For Measurement Of Aluminiummentioning

confidence: 99%

Uptake of aluminium by plant cells

1996

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SUMMARY The question of intracellular or extracellular primary lesion in the Al toxicity syndrome is unresolved. One of the crucial points for answering that question is quantification of Al fluxes across the plasma membrane within seconds or minutes of exposure of roots to Al, i.e. concurrent with or preceding the first symptoms of Al toxicity. A review of the available literature on Al uptake shows that there is abundant information on Al accumulation in root tissues only after the relatively prolonged uptake period, ranging from 30 min to over 24 h. Most of these reports either assume or claim explicitly that intracellular Al was measured, even though Al ions are bound strongly to negative charges in the apoplasm. Therefore, an effective and complete desorption of apoplasmic Al after the uptake period is crucial for measurements of intracellular Al. However, published studies do not seem to have desorbed cell‐wall‐bound Al appropriately. Recent studies with giant algal cells of Chara corallina, where physical separation of cell wall and cytoplasm after the uptake period can be achieved surgically, showed that desorption of Al from the apoplasm, even employing a wider variety of desorbents and more stringent conditions than in any of the previously published reports, cannot be achieved completely within 5 h. Consequently, measured rates of uptake of Al across the plasma membrane of intact Chara cells employing the cell wall/cytoplasm separation technique are up to several orders of magnitude lower than previously published values in which cell‐wall Al was attributed to the transmembrane uptake component. Although there is no doubt that Al does cross the plasma membrane, no information exists about which Al species or complexes take part in the transmembrane flux, mainly because of complexities inherent in Al speciation at the solution/ion exchanger (i.e. the cell wall and the membrane) interface. Similarly, it is not known which membrane transporters are involved in transport of Al across the plasma membrane. The Al‐resistant plant genotypes generally accumulate less Al in the root tips than do the Al‐sensitive genotypes. No direct relationship, however, appears to exist between increased organic acid extrusion as a mechanism of resistance to Al and decreased transmembrane flux of Al. Al‐accumulator plant species accumulate relatively large amounts of Al in their tissues without having a greater Al uptake rate than non‐accumulator genotypes. Progress in deciphering structural and functional aspects of transport of Al across the plasma membrane of intact plant cells will rely on using giant algal cells in which physical separation of the cell wall and the cytoplasm can be achieved, because, at present, there is no reliable quantitative method which can overcome problems presented by a relatively large apoplasmic Al pool remaining after desorption of intact root cells of higher plants.

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Section: In Situ Techniques For Measurement Of Aluminiummentioning

confidence: 99%

Uptake of aluminium by plant cells

1996

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“…This method is also at low cost, with respect to the other point-like methods as X-ray fluorescence spectroscopy, or neutron activation method. The method was used early in the metallurgy [3], inorganic material investigation [3,4], archeology and museum investigation [5,6,7], analysis of the paintings and sculptures [8,9], medicine and biology [10][11][12][13][14][15]. In the medical field it was studied the composition of the dental implants [10], of the super thin layers [10], of the bones in different regions [11], of hair [12], and so on.…”

Section: Laser Microspectral Analysis In Dentistrymentioning

confidence: 99%

Microleakage in dentistry: new methods for investigating the gaps in biomaterials

et al. 2008

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The method of laser micro spectral analysis is a method of punctual method of analysis, which permits to investigate small quantity of material, around 0.1 µg. This method permits to establish the content of atoms and molecules. The method permits to make semi-quantitative and quantitative analysis. By this method it is possible to establish trace elements, with concentration of order ppm (parts per million). For atomic UV spectral analysis, intense spectra are produced by the transitions of the metals. The halogens have poor spectra in the UV region. The method is low cost and can be made also with devices adapted for this kind of measurement. By this method we established the curves of concentration distribution for Ca, Mg, P and Zn. These distributions are different of the direction amalgam-nerve duct and on a perpendicular direction to the direction amalgam-nerve duct. We must underline that the method of laser micro spectral analysis gives the possibility to measure the infiltration of due substances in the porous regions of filled tooth.

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“…There are several methods which may be classified as LDI MS approaches that have unique names, including laser microprobe mass analysis (LAMMA) [45] and laser microprobe mass spectrometry (LMMS) [46]. For many years, LDI MS has been successfully applied to profiling the biochemical composition of different tissues and cells [47][48][49][50][51] with several examples of LDI MSI being used to examine nervous system-related specimens [35,52]. In one of these, Todd et al [35] demonstrated that the antipsychotic drug, clozapine, which interacted specifically with the 5-HT 2 receptors after its injection into the tail vein of a rat, accumulated in the striatum and cerebral cortex but not in the corpus callosum.…”

Section: Desi Ms Ldi Ms La-icp Ms and Maldi Ms Imagingmentioning

confidence: 99%

Mass Spectrometric Imaging of the Nervous System

Rubakhin¹,

Hatcher²,

Monroe³

et al. 2007

CPD

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Mass spectrometric imaging (MSI) integrates multiple fields of analytical and biomedical research with the goal of generating chemical maps that present the identity and location of the elements, molecules, and molecular complexes that comprise biological structures. Rapid advances in the development of MSI, which include a broad range of sampling and mass spectrometry strategies, allow the increasingly information-rich creation of chemical images of structurally complex tissues, individual cells, and even single chromosomes. Here we describe a variety of MSI techniques available to investigate the nervous system, with particular focus on the capability of MSI to examine both normal and diseased brain function. An important investigative tool, MSI offers tremendous potential in fundamental studies of brain chemistry, localization of pharmaceutical compounds, and the discovery of biomarkers for different neuropathologies.

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Laser microprobe mass analysis: A review of applications in the life sciences

Cited by 58 publications

References 137 publications

Uptake of aluminium by plant cells

Uptake of aluminium by plant cells

Microleakage in dentistry: new methods for investigating the gaps in biomaterials

Mass Spectrometric Imaging of the Nervous System

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