Microleakage in dentistry: new methods for investigating the gaps in biomaterials

Abstract

The method of laser micro spectral analysis is a method of punctual method of analysis, which permits to investigate small quantity of material, around 0.1 µg. This method permits to establish the content of atoms and molecules. The method permits to make semi-quantitative and quantitative analysis. By this method it is possible to establish trace elements, with concentration of order ppm (parts per million). For atomic UV spectral analysis, intense spectra are produced by the transitions of the metals. The halogens have poor spectra in the UV region. The method is low cost and can be made also with devices adapted for this kind of measurement. By this method we established the curves of concentration distribution for Ca, Mg, P and Zn. These distributions are different of the direction amalgam-nerve duct and on a perpendicular direction to the direction amalgam-nerve duct. We must underline that the method of laser micro spectral analysis gives the possibility to measure the infiltration of due substances in the porous regions of filled tooth.

Laser-Induced Breakdown Spectroscopy

“…According to Rehse et al, biomedical applications of LIBS can be broadly classified into two categories: (1) analysis of human clinical specimens (including hard tissue such as teeth and bones and soft tissue such as human hair and skin or tissue samples, human blood, or other fluid samples) and (2) analysis and detection of pathogenic microorganisms (e.g., bacteria, pollen, virus, fungus, yeasts) that can infect human subjects and cause disease. Table ,− summarizes the most recent LIBS biomedical applications.…”

Laser-induced breakdown spectroscopy (LIBS): a novel technology for identifying microbes causing infectious diseases

“…LIBS is a superb technology to investigate biological specimens listed above and has also proven its capability in forensic science (Miziolek et al 2006;Cremers and Radziemski, 2006;Pasquini et al 2007;Singh and Thakur, 2007;Gondal et al, 2016d, Almessiere et al, 2018. The most recent biomedical LIBS applications have been condensed in Table 1 (Sinescu et al 2008;Negrutiu et al 2008;Hamzaoui et al 2011;Singh et al 2008Singh et al , 2009aPathak et al 2011;Jaswal et al, 2016a, b;Anzano and Lasheras 2009;Yueh et al 2009;El-Hussein et al 2010;Wu et al 2008;Martin et al 2003Martin et al , 2005Melikechi et al 2008;Lee et al 2017;Samek et al 1999;Riberdy et al 2017). Figure 2 shows different LIBS applications to analyze different kinds of bacterial samples, plant samples, animal and human tissues, and skin samples to map of metal elements in tissues and to analyze bio minerals.…”

“…Figure 2 shows different LIBS applications to analyze different kinds of bacterial samples, plant samples, animal and human tissues, and skin samples to map of metal elements in tissues and to analyze bio minerals. Generally, high level interest in LIBS-based examination of biological materials is motivated by its native advantages including real-time analysis without any need for cumbersome sample preparation with In dentistry Teeth Ca, Mg, P, Zn, Cr, Co, and I Microleakage in dentistry; microleakage between infrastructure and veneer materials in dentures Sinescu et al 2008;Negrutiu et al 2008 Analysis of nails…”