Somatic embryogenesis, secondary somatic embryogenesis, and shoot organogenesis in Rosa

Li, Xiangqian; Krasnyanski, Sergei F.; Korban, Schuyler S.

doi:10.1078/0176-1617-00688

Cited by 67 publications

(31 citation statements)

References 16 publications

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“…This AMP has shown inhibition to all 12 tested plant pathogenic fungi, and exhibits a higher antimicrobial activity than RS-ns-LTP-like protein extracted from radish seeds (Terras et al 1992;Cammue et al 1995;Tassin et al 1998). Therefore, in this study, the Ace-AMP1 coding sequence has been selected as a candidate gene for introducing disease resistance into rose using an Agrobacterium-mediated transformation protocol recently developed in our laboratory (Li et al 2002a(Li et al , 2002b.…”

Section: Introductionmentioning

confidence: 97%

Transgenic rose lines harboring an antimicrobial protein gene, Ace-AMP1 , demonstrate enhanced resistance to powdery mildew ( Sphaerotheca pannosa )

Gasic

Cammue

et al. 2003

Planta

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An antimicrobial protein gene, Ace-AMP1, was introduced into Rosa hybrida cv. Carefree Beauty via Agrobacterium-mediated transformation. A total of 500 putative transgenic plants were obtained from 100 primary embryogenic calli co-cultivated with A. tumefaciens following selection on a regeneration medium containing 100 mg/l kanamycin. Polymerase chain reaction analysis of these putative transgenic lines, using primers for both Ace-AMP1 and neomycin phosphotransferase ( npt II) genes, showed that 62% of these plants were positive for both transgenes. These lines were further confirmed for stable integration of Ace-AMP1 and npt II genes by Southern blotting. Transcription of the Ace-AMP1 transgene in various transgenic rose lines was determined using Northern blotting. Transgenic rose lines inoculated with conidial spores of Sphaerotheca pannosa (Wallr.: Fr.) Lev. var. rosae showed enhanced resistance to powdery mildew using both a detached-leaf assay and an in vivo greenhouse whole-plant assay.

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Section: Introductionmentioning

confidence: 97%

Transgenic rose lines harboring an antimicrobial protein gene, Ace-AMP1 , demonstrate enhanced resistance to powdery mildew ( Sphaerotheca pannosa )

Gasic

Cammue

et al. 2003

Planta

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“…other plant species (Little et al, 2000;Li et al, 2002). Differentiation of the globular structures and the appearance of shoot apex and primordial leaves (Fig.…”

Section: Franklin and Diasmentioning

confidence: 99%

Organogenesis and embryogenesis in several Hypericum perforatum genotypes

Franklin

Dias

2006

In Vitro Cell. Dev. Biol. - Plant

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SummarySt John's wort (Hypericum perforatum) is a valuable plant used as a herbal remedy or in phytopharmaceutical drugs to treat a variety of physical ailments. Much research has been performed to study the biochemical production of secondary metabolites of in vitro cultured plants or organs. However, all of these studies have looked at the regeneration of plants from explants in only one genotype. In addition, no study has revealed the mechanism of plant regeneration in H. perforatum, i.e. organogenesis or somatic embryogenesis. We found that different genotypes Helos, Topas, Elixir, and Numi responded similarly to regeneration medium. The regeneration responses (i.e. callus, root, or shoot production) of identical explants from different genotypes were similar. However, the source of explant material (leaves, hypocotyls, and roots) from the same genotype had significant effects on the response to media and plant regeneration frequency. Using scanning electron microscopy and light microscopy, the progress of organogenesis and embryogenesis under similar culture conditions was recorded. Root segments were the most responsive explants, producing the maximum number of shoots per explant of all the genotypes.

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“…Such genotype specificity could also explain the differences observed in root formation, which was only noticed with landraces Ci3 and MB. Such variability among genotypes within a given species has been observed in the past in terms of callus proliferation and root formation ability in Primula vulgaris (Schween and Schwenkel, 2003) and in Rosa (Li et al, 2002). probably due to the use of inadequate hormones.…”

Section: Discussionmentioning

confidence: 99%

In vitro culture of Bambara groundnut [<i>Vigna subrerranea</i> (L.) Verdc., Favaceae ]: Effect of plant growth regulators, explant type and genotype on callus induction and differentiation

Koné¹,

Kouakou²,

Kone³

et al. 2009

Agronomie Africaine

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A technique was developed to induce callus formation and differentiation using leaf, petiole and root explants from Bambara groundnut [Vigna subterranea (L.) Verdc.]. The combinations and concentrations of different growth regulators were shown to be critical factors in the frequency of callus formation, as well as, in the potential for callus differentiation. It was established that the best responses in callus induction (75 %) were obtained from petiole on a MS (Murashige and Skoog, 1962) basal medium, supplemented with 3 -5 mg/l N-benzylaminopurine (BAP) + 0.5 mg/l α-naphthaleneacetic acid (NAA). Callus growth (GI) was more significant with Ci1 (3.52) and Ci4 (2.76) landraces, while root formation was especially observed with the Ci3 (24.81 %) landrace. Here, for the first time, induction of embryogenic and organogenic callus from petiole, leaf and root of Vigna subterranea (L.) was reported. Both adventitious buds and somatic embryos were initiated after 3 months of subculture on a callus induction medium containing 3-5mg/l BAP + 0.1 -0.5 mg/l ANA. Somatic embryogenesis (50 -68 %) occur more frequently than organogenesis (11 -27 %).Keywords : Callus, bambara groundnut (Vigna subterranea L.), somatic embryogenesis, organogenesis. Ci1 (3,52) et Ci4 (2,76) RESUME FACTEURS INFLUENÇANT L'INDUCTION ET LA DIFFERENCIATION DE CALS Un protocole a été développé pour induire la formation de cals, à partir des explants de feuille, de pétiole et de racine chez le pois de bambara [Vigna subterranea (L.)]. Les combinaisons et les concentrations de différents régulateurs de croissance se sont avérées être des facteurs déterminants dans la fréquence d'induction des cals, de même que pour le potentiel de différenciation des cals. Les résultats montrent également que l'induction des cals a été plus importante avec le pétiole (75 %) sur le milieu de base MS (Murashige et Skoog, 1962) supplémenté avec 3 -5 mg/l de la N-benzylaminopurine (BAP) + 0,5 mg/l de l'acide α-naphthalène acétique (ANA). La croissance des cals (IC) a été plus importante avec les écotypes 16M. KONE et al.

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Somatic embryogenesis, secondary somatic embryogenesis, and shoot organogenesis in Rosa

Cited by 67 publications

References 16 publications

Transgenic rose lines harboring an antimicrobial protein gene, Ace-AMP1 , demonstrate enhanced resistance to powdery mildew ( Sphaerotheca pannosa )

Transgenic rose lines harboring an antimicrobial protein gene, Ace-AMP1 , demonstrate enhanced resistance to powdery mildew ( Sphaerotheca pannosa )

Organogenesis and embryogenesis in several Hypericum perforatum genotypes

In vitro culture of Bambara groundnut [<i>Vigna subrerranea</i> (L.) Verdc., Favaceae ]: Effect of plant growth regulators, explant type and genotype on callus induction and differentiation

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