Somatic Embryogenesis: Polycomb Complexes Control Cell-to-Embryo Transition

Duarte-Aké, Fátima; Nic-Can, Geovanny I.; De‐la‐Peña, Clelia

doi:10.1007/978-3-030-14760-0_13

Cited by 9 publications

(7 citation statements)

References 67 publications

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“…Depending on the target lysine and the degree of methylation, activation, or inactivation of genes could be induced [42]. The most frequent histone methylation marks, H3K27me3 and H3K4me3, are catalyzed by, respectively, the Polycomb-group (PcG) and Trithorax-group (TrxG) proteins, of documented roles in regulating plant developmental plasticity, including stem cell maintenance, cellular reprogramming, and plant responses to environmental cues (reviewed in [20], [43]). The PcG proteins are classified into two complexes, POLYCOMB REPRESSIVE COMPLEX 1 and 2 (PRC1 and PRC2), which cooperate to repress the genes via histone methylation during plant development [44].…”

Section: Introductionmentioning

confidence: 99%

Epigenetic Regulation of Auxin-Induced Somatic Embryogenesis in Plants

Wójcikowska

Wójcik

Gaj

2020

IJMS

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Somatic embryogenesis (SE) that is induced in plant explants in response to auxin treatment is closely associated with an extensive genetic reprogramming of the cell transcriptome. The significant modulation of the gene transcription profiles during SE induction results from the epigenetic factors that fine-tune the gene expression towards embryogenic development. Among these factors, microRNA molecules (miRNAs) contribute to the post-transcriptional regulation of gene expression. In the past few years, several miRNAs that regulate the SE-involved transcription factors (TFs) have been identified, and most of them were involved in the auxin-related processes, including auxin metabolism and signaling. In addition to miRNAs, chemical modifications of DNA and chromatin, in particular the methylation of DNA and histones and histone acetylation, have been shown to shape the SE transcriptomes. In response to auxin, these epigenetic modifications regulate the chromatin structure, and hence essentially contribute to the control of gene expression during SE induction. In this paper, we describe the current state of knowledge with regard to the SE epigenome. The complex interactions within and between the epigenetic factors, the key SE TFs that have been revealed, and the relationships between the SE epigenome and auxin-related processes such as auxin perception, metabolism, and signaling are highlighted.

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Section: Introductionmentioning

confidence: 99%

Epigenetic Regulation of Auxin-Induced Somatic Embryogenesis in Plants

Wójcikowska

Wójcik

Gaj

2020

IJMS

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“…PRC1 recognizes H3K27me3 alterations and promotes chromatin compaction via histone H2A lysine ubiquitination ( Salaün et al, 2021 ). A recent study showed that PRC1 and PRC2 repress the expression of embryo-specific genes, including LAFL , AGL15 , WOX5 , BBM , and PIN1 ( Duarte-Aké et al, 2019 ). Additionally, pickle (PKL), a member of the chromodomain helicase DNA-binding protein 3 family of chromatin ATPase remodelers, is another epigenetic factor that plays a key role in preventing somatic cells from producing embryonic traits ( Ogas et al, 1999 ).…”

Section: Molecular Mechanisms Of Somatic Embryogenesismentioning

confidence: 99%

New Insights Into Tissue Culture Plant-Regeneration Mechanisms

et al. 2022

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Plant regeneration occurs when plants repair or replace damaged structures based on the totipotency and pluripotency of their cells. Tissue culture is one of the most widely used regenerative technologies. Recently, a series of breakthroughs were made in the study of plant regeneration. This review summarizes two regenerative pathways in tissue culture: somatic embryogenesis and de novo organogenesis. Furthermore, we review the environmental factors influencing plant regeneration from explant sources, basal culture medium, plant growth regulators, and light/dark treatment. Additionally, we analyse the molecular mechanisms underlying two pathways. This knowledge will promote an understanding of the fundamental principles of plant regeneration from precursor cells and lay a solid foundation for applying plant micropropagation and genetic modification.

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“…One of the interesting groups of proteins related to histone methylation marks that are involved in regulating plant cellular reprogramming are the Polycomb-group (PcG). (reviewed in Duarte-Aké et al, 2019 ). The PcG proteins are classified into two complexes, POLYCOMB REPRESSIVE COMPLEX 1 and 2 (PRC1 and PRC2), which are involved in repression of genes via histone methylation (Grossniklaus and Paro, 2014 ; Mozgová et al, 2015 ).…”

Section: Agl15 and Potential Links To Epigenetic Regulation Of Somati...mentioning

confidence: 99%

AGL15 Promotion of Somatic Embryogenesis: Role and Molecular Mechanism

et al. 2022

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Plants have amazing regenerative properties with single somatic cells, or groups of cells able to give rise to fully formed plants. One means of regeneration is somatic embryogenesis, by which an embryonic structure is formed that “converts” into a plantlet. Somatic embryogenesis has been used as a model for zygotic processes that are buried within layers of maternal tissues. Understanding mechanisms of somatic embryo induction and development are important as a more accessible model for seed development. We rely on seed development not only for most of our caloric intake, but also as a delivery system for engineered crops to meet agricultural challenges. Regeneration of transformed cells is needed for this applied work as well as basic research to understand gene function. Here we focus on a MADS-domain transcription factor, AGAMOUS-Like15 (AGL15) that shows a positive correlation between accumulation levels and capacity for somatic embryogenesis. We relate AGL15 function to other transcription factors, hormones, and epigenetic modifiers involved in somatic embryo development.

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Somatic Embryogenesis: Polycomb Complexes Control Cell-to-Embryo Transition

Cited by 9 publications

References 67 publications

Epigenetic Regulation of Auxin-Induced Somatic Embryogenesis in Plants

Epigenetic Regulation of Auxin-Induced Somatic Embryogenesis in Plants

New Insights Into Tissue Culture Plant-Regeneration Mechanisms

AGL15 Promotion of Somatic Embryogenesis: Role and Molecular Mechanism

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