Somatic embryogenesis of sago palm (<i>Metroxylon sagu</i> Rottb.) from different origins in Indonesia

Sinta, Masna Maya; Riyadi, Imron; Sumaryono, Sumaryono

doi:10.1088/1755-1315/183/1/012008

Cited by 3 publications

(3 citation statements)

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“…In this experiment, plastic wrap was used as culture closures. Based on the experiment by Sinta, Riyadi, & Sumaryono (2011), the use of plastic wrap as culture closures provided the best aeration in the in vitro culture of oil palm. It made gas exchange from the inside to the outside of culture vessels to be more intense, so that the moisture could be reduced.…”

Section: Survival Ratementioning

confidence: 99%

In Vitro Propagation of Anthurium adreanum cv. Nitta through Organogenesis

Saptari¹,

Sinta²,

Budiani³

2017

Agrivita.J.Agr.Sci

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Anthurium adreanum Lindl. cv. Nitta is an ornamental plant for cut flower industries. In vitro propagation enables a large scale production of high quality seedlings of A. adreanum quickly. The aim of this experiment was to overcome some problems in the in vitro propagation of A. adreanum, such as contamination, lack of plantlets vigor and low rate of survival in the acclimatization. In vitro propagation of A. adreanum was conducted via organogenesis from leaf explant. Explant sterilization using desogerm, antioxidant, alcohol, rifampicin, and NaOCl was the best because they reduced browning and contamination until 0 %. Organogenesis of A. adreanum was successfully initiated using MS medium added with 2,4-D and BAP at 1 mg L -1 , which produced 74 shoots per explant. Shoots were best maturated in MS medium with a half strength of macro minerals added with 1 mg L -1 paclobutrazol. Survival rate increased by 7 and 14 days hardening treatment using double layer medium in the light culture room. Survival rate reached 89.3 % at 8 weeks after acclimatization.

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Section: Survival Ratementioning

confidence: 99%

In Vitro Propagation of Anthurium adreanum cv. Nitta through Organogenesis

Saptari¹,

Sinta²,

Budiani³

2017

Agrivita.J.Agr.Sci

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“…Selisih berkas cahaya dalam botol dengan cahaya luar yaitu sekitar 0,82-1,09 µmol foton/m 2 /detik. Penelitian yang dilaporkan oleh Sinta et al (2011) pada planlet kelapa sawit dengan penggunaan tutup botol yang berbeda menunjukkan adanya selisih intensitas cahaya antara di luar dan di dalam botol sebesar 1,7-8,7 µmol foton/m 2 /detik. Semakin kecil selisih antara intensitas cahaya di luar dan di dalam botol, maka semakin banyak jumlah foton yang tersedia di Tabel 2.…”

Section: Intensitas Cahaya DI Dalam Botol Kulturunclassified

Modifikasi sistem kultur in vitro untuk meningkatkan vigor planlet stevia (Stevia rebaudiana Bert.) [Modification of in vitro culture system to increase the vigor of stevia (Stevia rebaudiana Bert.) plantlets]

Saptari¹,

Sumaryono²

2017

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Stevia (Stevia rebaudiana Bert.), a sweetener plant, has been mass propagated by tissue culture technique. Optimal conditions to increase vigor of stevia plantlets are needed to support the sustainability of in vitro plantlet stocks and increase plantlet survival rate during acclimatization. The aim of this research was to investigate the effect of different media, culture vessel sizes, and vessel closure types on the vigor of stevia plantlets. The plant material was derived from apical shoot cuttings of sterile stevia plantlets grown on WP medium without growth regulator. Several treatments used in this study were solid or double layer media; short or tall culture vessel; and polypropile screw cap or plastic film closures. Growth of plantlets was determined after 3 weeks of culture. Temperature and light intensity inside the vessels were also observed. The results showed that the best treatment to increase the vigor of stevia plantlets was a double-layer medium in a tall culture vessel (diameter 7 cm and height 11 cm) with either screw cap or plastic film. It was exhibited by significantly bigger stem diameter, more and bigger leaves, longer roots, and higher biomass fresh weight than those of other treatments. Higher temperature was observed on tall culture vessel, whereas all treatments did not significantly affect light intensity inside the vessels.[Keywords: stevia, plantlet vigor, double-layer medium, culture vessel size, vessel closure]AbstrakStevia (Stevia rebaudiana Bert.), tanaman pemanis, telah diperbanyak melalui teknologi kultur jaringan. Kondisi kultur optimal untuk meningkatkan vigor planlet stevia masih diperlukan untuk mendukung keberlanjutan tanaman stock in vitro dan untuk meningkatkan daya hidup planlet ketika diaklimatisasi. Penelitian yang dilakukan bertujuan untuk menentukan pengaruh penggunaan jenis media, ukuran botol kultur, dan jenis penutup botol yang berbeda terhadap vigor planlet stevia. Material tanaman yang digunakan didapat dari potongan tunas apikal plantlet stevia steril yang ditumbuhkan pada media WP tanpa zat pengatur tumbuh. Perlakuan jenis media terdiri atas media padat dan media dua-lapis (double-layer), ukuran botol pendek dan tinggi, serta jenis tutup ulir berbahan polipropilen dan lembaran plastik transparan. Pengamatan pertumbuhan planlet dilakukan setelah 3 minggu, juga dilakukan pengamatan terhadap suhu dan intensitas cahaya di dalam botol kultur. Hasil penelitian memperlihatkan bahwa perlakuan terbaik untuk meningkatkan vigor planlet stevia adalah dengan menggunakan media dua-lapis dalam botol kultur (diameter 7 cm, dan tinggi 11 cm), baik dengan tutup ulir maupun plastik. Hal ini ditunjukkan dari diameter batang lebih besar, daun lebih banyak dan besar, akar lebih panjang, serta bobot segar biomassa lebih tinggi dibandingkan dengan perlakuan lainnya. Suhu lebih tinggi terukur pada perlakuan botol tinggi, sedangkan semua perlakuan tidak mempengaruhi secara nyata intensitas cahaya di dalam botol kultur.[Kata kunci: stevia, vigor, media dua-lapis, ukuran botol kultur, tutup botol]

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“…attention and find the solutions to solve these problems and make appropriate interventions in solving it, so that the development process can be carried out well and have a positive impact on the people in Papua. Collaboration of various parties in the development process in Papua is an important matter that must be considered and planned properly by the government and other stakeholders that are directly related to this case.Environmental Aspects of Development inPapuaThe environment is another aspect that must be considered in the development ofPapua, where the land of Papua is known as a land that is rich in various types of resources and has a strong cultural environment(Muchtar & Yunus, 2019;Sinta et al, 2018;Tambaip et al, 2020). Currently, environmental problems are also a challenge for development in Papua, where deforestation and environmental destruction are still common in some areas(Abood et al, 2015;Brockhaus et al, 2014;Krystof Obidzinski et al, 2012;Suryatmojo et al, 2019).…”

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confidence: 99%

The Direction of Papua Development: Is A New Autonomous Region the Answer?

Fauzi¹,

Mayor

Liauw

2023

pgr

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This study aims to show what kind of problems and challenges occur in the middle of Papua Province development, as well as the extent of complexity of these development problems and the challenges of the New Autonomous Region to answer it. We use the systematic literature review method by showing a collection of literature from the Scopus database that discusses the development of Papua and analyzing the literature collection into several clusters. Bibliometric analysis is used to generate statistical models that show the relationship between each study. 151 literature sources were used in this study and produced three clusters namely development process, environment, and communication. From these aspects, several development efforts have been carried out by the government such as collaboration with the private sector, community capacity building through local value development, and the involvement of indigenous peoples. Moreover, there are several things that need to be considered such as land acquisition issues, the difficulty of access, planning and control functions, political intervention and interests, along with the communication system that has not been integrated. The New Autonomous Region can have a narrow span of control so it will optimize supervision and monitoring functions, but there are several things that need to be considered when implementing it such as planning, existing human resources capacity, and financial aspects. There are some limitations in this paper, where we cannot reach some highland areas to directly observe the challenges that occur there. Future research can develop discussions about Papua development through direct observations in those areas.

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Somatic embryogenesis of sago palm (Metroxylon sagu Rottb.) from different origins in Indonesia

Cited by 3 publications

References 0 publications

In Vitro Propagation of Anthurium adreanum cv. Nitta through Organogenesis

In Vitro Propagation of Anthurium adreanum cv. Nitta through Organogenesis

Modifikasi sistem kultur in vitro untuk meningkatkan vigor planlet stevia (Stevia rebaudiana Bert.) [Modification of in vitro culture system to increase the vigor of stevia (Stevia rebaudiana Bert.) plantlets]

The Direction of Papua Development: Is A New Autonomous Region the Answer?

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