In Vitro Propagation of Anthurium adreanum cv. Nitta through Organogenesis

Abstract

Anthurium adreanum Lindl. cv. Nitta is an ornamental plant for cut flower industries. In vitro propagation enables a large scale production of high quality seedlings of A. adreanum quickly. The aim of this experiment was to overcome some problems in the in vitro propagation of A. adreanum, such as contamination, lack of plantlets vigor and low rate of survival in the acclimatization. In vitro propagation of A. adreanum was conducted via organogenesis from leaf explant. Explant sterilization using desogerm, antioxidant, alcohol, rifampicin, and NaOCl was the best because they reduced browning and contamination until 0 %. Organogenesis of A. adreanum was successfully initiated using MS medium added with 2,4-D and BAP at 1 mg L -1 , which produced 74 shoots per explant. Shoots were best maturated in MS medium with a half strength of macro minerals added with 1 mg L -1 paclobutrazol. Survival rate increased by 7 and 14 days hardening treatment using double layer medium in the light culture room. Survival rate reached 89.3 % at 8 weeks after acclimatization.

Evaluation of Culture Media for In Vitro Conservation of Gladiolus Cultivars

“…In addition, tissue culture systems are considered safe for germplasm exchange in international schemes. A smaller sample size has made the cultures can be transferred in sterile conditions (Rodrigues, Arruda, & Forti, 2018;Saptari, Sinta, & Budiani, 2017).…”

The in vitro multiplication of anthurium cv. Eidibel

“…Several protocols are available in the scientific literature for the production of micropropagated plantlets of Anthurium andraeanum via organogenesis (ISLAM et al, 2010;GU et al, 2012;CARDOSO;HABERMANN, 2014;AJDARBIN et al, 2015;BHATTACHARYA et al, 2015;PERERA et al . ;2016;GUADALUPE;MAYANIN, 2017;PRAKASHA et al, 2017;SAPTARI et al, 2017;THOKCHOM;MAITRA, 2017;ZHANG et al, 2017). Silva et al (2015) state that the MS growth medium (MURASHIGE;SKOOG, 1962) is the most often used in the in vitro propagation of anthurium.…”

“…Na literatura científica estão disponíveis vários protocolos para a produção de mudas micropropagadas de Anthurium andraeanum pela via organogênica (ISLAM et al, 2010;GU et al, 2012;CARDOSO;HABERMANN, 2014;AJDARBIN et al, 2015;BHATTACHARYA et al, 2015;PERERA et al;2016;GUADALUPE;MAYANIN, 2017;PRAKASHA et al, 2017;SAPTARI et al, 2017;THOKCHOM;MAITRA, 2017;ZHANG et al, 2017). Silva et al (2015) mencionam que o meio de cultivo MS (MURASHIGE;SKOOG, 1962) é o mais utilizado como meio basal na propagação in vitro de Anthurium.…”

“…The in vitro multiplication of anthurium cv. Eidibel Among the cytokinins used during the multiplication phase in the production of micropropagated anthurium plantlets, 6-benzylaminopurine (BAP) has been mentioned as the most efficient for shoot regeneration at concentrations ranging from 0.27 µM to 25.00 µM ( ISLAM et al, 2010;GU et al, 2012;CARDOSO;HABERMANN, 2014;AJDARBIN et al, 2015;BHATTACHARYA et al, 2015;PERERA et al, 2016;GUADALUPE;MAYANIN, 2017;PRAKASHA et al ., 2017;SAPTARI et al, 2017;THOKCHOM;MAITRA, 2017;ZANG et al, 2017;BHAVANA et al, 2018).…”

“…Studies on the production of micropropagated plantlets of anthurium 'Eidibel' are scarce, with recorded multiplication rates of from 3.95 to 6.11 (PINHEIRO et al, 2009). In addition, although the photoperiod of 12 h is used for the in vitro production of anthurium plantlets (GU et al, 2012;SAPTARI et al, 2017, ZANG et al, 2017, the most common is the photoperiod of 16 hours (ISLAM et al, 2010;CARDOSO;HABERMANN, 2014;AJDARBIN et al, 2015;BHATTACHARYA et al, 2015;GUADALUPE;MAYANIN, 2017;THOKCHOM;MAITRA, 2017;BHAVANA et al, 2018). The aim of this study, therefore, was to determine the most suitable relationship between growth medium, BAP concentration and photoperiod for increasing the in vitro multiplication rate of the 'Eidibel' cultivar.…”