Somatic embryogenesis, acclimatization and genetic homogeneity assessment of regenerated plantlets of Anoectochilus elatus Lindl., an endangered terrestrial jewel orchid

Sherif, N. Ahamed; Benjamin, J. H. Franklin; Kumar, T. Senthil; Rao, M. S.

doi:10.1007/s11240-017-1330-4

Cited by 50 publications

(25 citation statements)

References 47 publications

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“…This result supports the conclusion that the regenerated CCN51 and TSH565 plants produced via somatic embryogenesis had no variations with respect to field plants. Similar results, where a genetic homogeneity of close to 100%, have been obtained from other species derived from somatic embryogenesis, both directly and indirectly, from Hibiscus sabdariffa L (Konar et al, 2018), Abutilon indicum L. (Seth et al, 2017), Cucumis melo L. (Raji et al, 2018), Anoectochilus elatus Lindl (Sherif et al, 2018), Citrullus lanatus (Vinoth and Ravindhran, 2016) and Bacopa monnieri (Largia et al, 2015).…”

Section: Discussionsupporting

confidence: 82%

“…Plant tissue culture techniques cause genetic instability due to variations in ploidy level, point mutations, or translocations, leading to somaclonal variation, which occurs by using various growth regulators in the medium (Largia et al, 2015). Plants regenerated as explants using axillary buds, and meristematic tissue has shown the lowest genetic variation between 0-10% (Sherif et al, 2018;Vinoth and Ravindhran, 2016). However, many authors reported that plants regenerated through somatic embryogenesis exhibit genetic uniformity and integrity (Rai et al, 2012), some reports substantiate the presence of genetic modification in plants derived from somatic embryos (Viehmannova et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Determination of Genetic Stability in Cacao Plants (Theobroma Cacao L.) Derived from Somatic Embryogenesis Using Microsatellite Molecular Markers (SSR)

Ramírez

Duque

Calle-Tobón

et al. 2021

International Journal of Fruit Science

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The clonal propagation of T. cacao by somatic embryogenesis (SE) is a promising approach to multiply elite genotypes. Assessing clonal fidelity in plants regenerated from somatic embryos is the first step toward ensuring genetic uniformity in the mass production of planting material. This study assessed the genetic stability of cacao plantlets propagated by SE and conventional grafting for genotypes CCN51 and TSH565 using 13 SSR. The leaves of in vitro plantlets (IVL) were collected from 6-month-old plants and leaves of field plants (FPL) were selected from 3-year-old trees. The 13 analyzed loci revealed 25 alleles in genotype CCN51 and 24 alleles in genotype TSH565. The highest PIC value was observed for all SSR, only mTcCIR8 and mTcUNICAMP09 were intermediate, with PIC values of less than 0.250. IVL and FPL populations were genetically equal. According to the results, no differences in allelic composition were observed between FPL and IVL in each genotype. This indicates that plants propagated by SE did not show perceptible detriment to their genome with the used SSR. In addition, Jaccard's coefficient showed more than a 91% similarity for TSH565 and 92% for CCN51. The UPGMA and PCA showed that the populations tended to group within two genotypes. The SSR results obtained do not exclude the occurrence of other changes in the nuclear genome. However, considering the morphological stability of in vitro propagated plants, the results indicate that the protocol used is suitable and efficient for large scale, true-to-type propagation of genotypes CCN51 and TSH565 for commercial purposes.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Determination of Genetic Stability in Cacao Plants (Theobroma Cacao L.) Derived from Somatic Embryogenesis Using Microsatellite Molecular Markers (SSR)

Ramírez

Duque

Calle-Tobón

et al. 2021

International Journal of Fruit Science

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“…Somaclonal variation or genetic instability in plants could potentially be caused by long-term in vitro storage of plant germplasm (Sherif et al, 2018). Molecular markers such as amplified fragment length polymorphism (AFLP), RAPD, ISSRs, and start codon targeted (SCoT) all have been used to assess the genetic stability of in vitro versus in vivo plants (Bhattacharyya and Kumaria, 2015;Khilwani et al, 2016;Milella et al, 2011;Seth et al, 2017;Sherif et al, 2018). Some previous studies have reported 100% homogeneity among plantlets regenerated from somatic embryos.…”

Section: Resultsmentioning

confidence: 99%

Germination of Persian Walnut Somatic Embryos and Evaluation of their Genetic Stability by ISSR Fingerprinting and Flow Cytometry

Sadat-Hosseini¹,

Vahdati²,

Leslie³

2019

horts

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Somatic embryos (SEs) can play important roles in genetic manipulation and breeding. They can be used as targets for induced mutagenesis, as material for cryopreservation and germplasm conservation, and for transformation or gene editing in support of plant improvement and proof of gene function. However, germination rates of walnut (Juglans regia) SEs are low, and the genetic stability of plantlets regenerated from them has not been explored. Here, we studied first the effects of gibberellic acid (GA3) and low temperature storage (LTS) on germination of walnut somatic embryos. Second, we assessed the genetic fidelity of plantlets regenerated from these SEs by comparing them to each other and to their cultivar of origin. Results showed that GA3 and LTS increased the walnut SE germination rate. The best rate was observed when SEs were subjected to LTS for 60 d followed by culture on a medium with either 1 or 3 mg·L−1 GA3 (56.6% and 46.6% germination respectively). Genetic stability was evaluated, using flow cytometry and 15 sets of ISSR primers. Flow cytometry indicated that all samples (i.e., regenerated and parental counterpart) showed the same peak. Amplified fragments of inter simple sequence repeats (ISSR) primers ranged in size from ≈200 to 1800 bp. All ISSR profiles of regenerants were monomorphic. Results did not show any genetic differences among plantlets regenerated from SEs or from their parental counterpart. Due to this apparent genetic stability, walnut SEs can be useful for genetic transformation and germplasm conservation.

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“…The elimination of browning associated with in vitro culture is vital for successfully establishing cultures [ 24 ]. The PGR with the highest efficiency is TDZ, a phenyl urea derivative considered as the current number-one-ranking synthetic cytokinin for regeneration and somatic embryogenesis [ 27 , 28 ]. Our observations are in line with high-cytokinin emerging species beneficial for the somatic embryogenesis induction [ 21 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

A Novel and Efficient In Vitro Organogenesis Approach for Ajuga lupulina Maxim

Yang

et al. 2021

Plants

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This work was aimed at establishing an effective approach for in vitro propagation of Ajuga lupulina Maxim, a medicinal and ornamental plant mainly found in eastern Xizang, in the western Sichuan region of China. We report an optimum response in the proliferation of axillary shoots from nodal segment explants (10.2 shoots/explant) on MS medium containing 3.0 mg L−1 of 6-benzyladenine (BA). When BA and TDZ individually or in combination with NAA were employed for adventitious shoot regeneration, shoots and embryo-like structures (ELSs) were noted in the callus from leaf explants. The maximum response of 26.4 shoots /explant (81.6%) and 12.0 ELSs/explant were ascertained on MS medium with 4.0 mg L−1 TDZ and 0.1 mg L−1 NAA. The leaf despite browning still demonstrated a high regeneration capacity. TDZ (2.0 mg L−1) and BA (2.0 mg L−1) along with NAA (0.01 mg L−1) were found to perform well for shoot regeneration via callus from shoot tip explants. The best for rooting was MS medium (half-strength) containing indole-3-butyric acid (IBA: 1.5 mg L−1) and (NAA: 0.5 mg L−1) with the maximum number of roots (25.8 per shoot) and the highest rooting frequency (81.71%). The survival of the plantlets in the greenhouse was 78.2% indicative of successful acclimatization. This work is the first report of a consistent, definitive, and unique protocol for A. lupulina regeneration, paving the way for the in vitro preservation of such significant genetic resources and also further allied systems based on such callus-based or embryo-based approaches.

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Somatic embryogenesis, acclimatization and genetic homogeneity assessment of regenerated plantlets of Anoectochilus elatus Lindl., an endangered terrestrial jewel orchid

Cited by 50 publications

References 47 publications

Determination of Genetic Stability in Cacao Plants (Theobroma Cacao L.) Derived from Somatic Embryogenesis Using Microsatellite Molecular Markers (SSR)

Determination of Genetic Stability in Cacao Plants (Theobroma Cacao L.) Derived from Somatic Embryogenesis Using Microsatellite Molecular Markers (SSR)

Germination of Persian Walnut Somatic Embryos and Evaluation of their Genetic Stability by ISSR Fingerprinting and Flow Cytometry

A Novel and Efficient In Vitro Organogenesis Approach for Ajuga lupulina Maxim

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