2021
DOI: 10.3390/plants10091918
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A Novel and Efficient In Vitro Organogenesis Approach for Ajuga lupulina Maxim

Abstract: This work was aimed at establishing an effective approach for in vitro propagation of Ajuga lupulina Maxim, a medicinal and ornamental plant mainly found in eastern Xizang, in the western Sichuan region of China. We report an optimum response in the proliferation of axillary shoots from nodal segment explants (10.2 shoots/explant) on MS medium containing 3.0 mg L−1 of 6-benzyladenine (BA). When BA and TDZ individually or in combination with NAA were employed for adventitious shoot regeneration, shoots and embr… Show more

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Cited by 4 publications
(5 citation statements)
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References 33 publications
(57 reference statements)
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“…It is reported that hyperhydricity is related to cytokinin concentration and type [23][24][25]. In the present study, Kin causes shoot hyperhydration, this result aligning with our previous discoveries on Ajuga lupulina Maxim [26].…”
Section: Effect Of Pgrssupporting
confidence: 92%
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“…It is reported that hyperhydricity is related to cytokinin concentration and type [23][24][25]. In the present study, Kin causes shoot hyperhydration, this result aligning with our previous discoveries on Ajuga lupulina Maxim [26].…”
Section: Effect Of Pgrssupporting
confidence: 92%
“…Although the aseptic seeding leaves brown easily, they still sustained high differentiation capacity. The phenomenon has been reported in species of Ajuga lupulina [26]. We suspect that light intensity or light quality may cause browning, but this is not enough to adversely affect the differentiation ability of leaf explants.…”
Section: Leaf As Explant Source For Regenerationmentioning
confidence: 56%
“…It is interesting that compound 16 was firstly isolated from the nature. Five abietane diterpenoids (7)(8)(9)(10)(11) show strong inhibitory ferroptosis in HT22 cells.…”
Section: Discussionmentioning
confidence: 97%
“…[5] The folk usage of the whole plants was to address hypertension, epilepsy, sore throat, and urinary calculi. [6,7] It is reported that the plants contain abundant bioactive metabolites, including diterpenoids, steroids, iridoids, and flavonoids, which have extensive biological effects, such as antioxidative, insect anti-feed, cytotoxic, and antibacterial activities. [6,8] However, the anti-ferroptosis activity of constituents from A. lupulina has not been reported yet.…”
Section: Introductionmentioning
confidence: 99%
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