Solvent system selectivities in countercurrent chromatography using Salicornia gaudichaudiana metabolites as practical example with off-line electrospray mass-spectrometry injection profiling

Preparative mass‐spectrometry profiling of minor concentrated metabolites in Salicornia gaudichaudiana Moq by high‐speed countercurrent chromatography and off‐line electrospray mass‐spectrometry injection

“…In a previous study , to help HSCCC solvent system selectivity, evaluation, and rapid identification of main compounds, obtained fractions were analyzed by off‐line ESI‐MS/MS. The methodology allowed distinguishing six major compounds: kaempferol‐7‐O‐ β ‐D‐glucopyranoside, quercetin‐3‐O‐ β ‐D‐galactopyranoside, isorhamnetin‐3‐O‐ β ‐D‐galactopyranoside, isomeric dicaffeoylquinic acids, isomeric dihydrocaffeoyl ‐ caffeoyl ‐ quinic acids and dihydrocaffeoyl ‐ caffeoyl ‐ feruloyl ‐ quinic acid.…”

“…The early study on S. gaudichaudiana EtOAcExt major compounds was used to guide the identification of the minor derivatives. Previously isolated compounds from Salicornia genus were listed with its molecular weights and fragmentation pattern as chemotaxonomic database.…”

“…Solvent system selectivity tests and its evaluation were taken from previous work . The system n ‐hexane/ethylacetate/methanol/water at ratio 0.5:6.0:0.5:6.0 v/v was employed for the preparative separation of 500 mg of EtOAcExt.…”

“…Salicornia has a long history of applications in human diet and recently has been introduced in the European market as a vegetable named ‘samphire’, ‘green asparagus’, or ‘sea asparagus’, not only for its salty taste, but also for its high nutritional value . Since then a number of studies have been carried out on its chemical composition .…”

“…Because of the increasing interest in Salicornia species for its valuable commercial products, minor compounds of S. gaudichaudiana were investigated by a combination of semi‐preparative countercurrent chromatography (CCC) with off‐line injection electrospray mass‐spectrometry (ESI‐MS/MS) metabolite detection. This approach was successfully applied to evaluate the effects of CCC solvent system selectivity for improved isolation protocol of S. gaudichaudiana Moq major compounds .…”