The 8th International Conference on Counter-current Chromatography held at Brunel University, London, UK, July 23–25, 2014

Ignatova, Svetlana; Sutherland, Ian

doi:10.1016/j.chroma.2015.10.096

Cited by 34 publications

(4 citation statements)

References 30 publications

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“…Equation (20) describes the elution profile of a compound K D after the sample, containing Q = x s F τ s amount of the compound, which has been introduced into the column with a feed stream (x s is the compound concentration in the feed stream; τ s is the sample loading time).…”

Section: Conventional Elution Modementioning

confidence: 99%

“…It should be recalled that Equations (20) and (21) are expressed in normalized form by using the average concentration of the compound in the column and the mean residence time (the mean time for of the compound K D =1): The MDM CCC separations are carried out as described above in Section 2.3. The only difference is in sample loading conditions: instead of a pulse injection, the sample solution is introduced into a column during a certain time.…”

Section: Conventional Elution Modementioning

confidence: 99%

“…To address this issue, new, highly efficient separation and purification methods are being developed. Countercurrent chromatography (CCC) [9][10][11][12][13][14][15][16][17][18][19][20][21] is a separation technology that due to the combination of the features of liquid-liquid extraction and partition chromatography [22][23][24][25][26] offers a variety of operating modes and allows for high adaptability to different separation tasks. These operating modes, many of which can be implemented with minor changes of the equipment used in elution, make it possible to significantly improve the separation performance compared to the conventional isocratic batch injection mode.…”

Section: Introductionmentioning

confidence: 99%

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Analytical, Preparative, and Industrial-Scale Separation of Substances by Methods of Countercurrent Liquid-Liquid Chromatography

2020

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Countercurrent liquid-liquid chromatographic techniques (CCC), similar to solvent extraction, are based on the different distribution of compounds between two immiscible liquids and have been most widely used in natural product separations. Due to its high load capacity, low solvent consumption, the diversity of separation methods, and easy scale-up, CCC provides an attractive tool to obtain pure compounds in the analytical, preparative, and industrial-scale separations. This review focuses on the steady-state and non-steady-state CCC separations ranging from conventional CCC to more novel methods such as different modifications of dual mode, closed-loop recycling, and closed-loop recycling dual modes. The design and modeling of various embodiments of CCC separation processes have been described.

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Section: Conventional Elution Modementioning

confidence: 99%

Section: Conventional Elution Modementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analytical, Preparative, and Industrial-Scale Separation of Substances by Methods of Countercurrent Liquid-Liquid Chromatography

2020

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“…This can be simply explained by a wider distribution of CCC instruments across the international research community, especially in China, and their lower cost and ease of manufacture. There is almost equal number of CPC and CCC instruments used in industry these days but access to their applications is rather limited (Ignatova and Sutherland 2015). Out of 22 common solvent systems for PhGs separations with CCC, 64% were ethyl acetate-nbutanol-water family with ratios depending on anatomical part of a plant (Fig.…”

Section: Ccs Application To Phenylethanoid Glycosides and Iridoids Pumentioning

confidence: 99%

An overview of the two-phase solvent systems used in the countercurrent separation of phenylethanoid glycosides and iridoids and their biological relevance

2019

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Phenylethanoid glycosides (PhGs) and iridoids are two vast groups of water soluble secondary metabolites widely distributed in plant kingdom and well known for their important biological activities. Their purification by conventional chromatography is time consuming, uses large amounts of organic solvents and requires repeated steps. Moreover, the sample recovery is low, because the hydroxyl groups of PhGs and iridoids make them strongly adsorbed onto the solid support (silica gel, Sephadex LH-20) during separation. Being a liquid-liquid based technology, countercurrent separation (CCS) comes as an alternative tool to overcome the issues associated with solid-phase adsorbents. It has been successfully used for the separation of many groups of specialized plant metabolites. This is the first extensive review describing the application of CCS for purification of iridoids and PhGs, based on the research papers from the last 20 years (1998-2018) that used countercurrent chromatography and centrifugal partition chromatography technologies. In total, 65 papers described the isolation of 84 different phytochemicals (28 PhGs and 56 iridoids) from 42 plant species belonging to 16 distinct families that were separated with 59 different biphasic solvent systems. Since PhGs and iridoids are highly polar molecules, more than half of the employed systems (56%) were composed of various ratios of ethyl acetate-alcohol-water, as well as binary systems, such as ethyl acetate-water and n-butanolwater. The current review may be used as starting point for CCS users on their sinuous road of isolating known or waiting to be discovered PhGs and iridoids.

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Continuous Synthesis and Purification by Coupling a Multistep Flow Reaction with Centrifugal Partition Chromatography

et al. 2017

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Continuous-flowm ultistep synthesis is combined with quasi-continuous final-product purification to produce pure products from crude reaction mixtures.I nt he nucleophilic aromatic substitution of 2,4-difluoronitrobenzene with morpholine followed by aheterogeneous catalytic hydrogenation, the desired monosubstituted product can be continuously separated from the co-and by-products in apurity of over 99 % by coupling af low reactor sequence to am ultiple dual-mode (MDM) centrifugal partition chromatography (CPC) device. This purification technique has many advantages over HPLC, such as higher resolution and no need for column replacement or silica recycling,a nd it does not suffer from irreversible adsorption.

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The 8th International Conference on Counter-current Chromatography held at Brunel University, London, UK, July 23–25, 2014

Cited by 34 publications

References 30 publications

Analytical, Preparative, and Industrial-Scale Separation of Substances by Methods of Countercurrent Liquid-Liquid Chromatography

Analytical, Preparative, and Industrial-Scale Separation of Substances by Methods of Countercurrent Liquid-Liquid Chromatography

An overview of the two-phase solvent systems used in the countercurrent separation of phenylethanoid glycosides and iridoids and their biological relevance

Continuous Synthesis and Purification by Coupling a Multistep Flow Reaction with Centrifugal Partition Chromatography

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