Solvent Extraction and GC-MS Analysis of Sesame Seeds for Determination of Bioactive Antioxidant Fatty Acid/Fatty Oil Components

Qadir, Abdul; Ali, Athar; Arif, Muhammad; Alrohaimi, Abdulmohsen H; Singh, Satya Prakash; Ahmad, Usama; Khalid, Mohd Ikhmal Hanif Abdul; Kumar, Arun

doi:10.1055/s-0043-123466

Cited by 24 publications

(10 citation statements)

References 10 publications

(8 reference statements)

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“…Acylglycerols provide a key energy reserve in many organisms and are some of the common components of many seed oils [57][58][59] , with TAGs being the most common acylglycerol in such oils 21 . In mature seeds, the TAGs are stored in lipid bodies surrounded by oleosin proteins that occupy close to 60% of the cell volume of the cotyledons 60 .…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analyses reveals the dynamic nature of oil accumulation during seed development of Plukenetia volubilis L.

Guo

Yan

et al. 2020

Sci Rep

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Sacha inchi (Plukenetia volubilis L.) is a shrub native to Amazon rainforests that’s of commercial interest as its seeds contain 35–60% edible oil (dry weight). This oil is one of the healthiest vegetable oils due to its high polyunsaturated fatty acid content and favourable ratio of omega-6 to omega-3 fatty acids. De novo transcriptome assembly and comparative analyses were performed on sacha inchi seeds from five stages of seed development in order to identifying genes associated with oil accumulation and fatty acid production. Of 30,189 unigenes that could be annotated in public databases, 20,446 were differentially expressed unigenes. A total of 14 KEGG pathways related to lipid metabolism were found, and 86 unigenes encoding enzymes involved in α-linolenic acid (ALA) biosynthesis were obtained including five unigenes encoding FATA (Unigene0008403), SAD (Unigene0012943), DHLAT (Unigene0014324), α-CT (Unigene0022151) and KAS II (Unigene0024371) that were significantly up-regulated in the final stage of seed development. A total of 66 unigenes encoding key enzymes involved in the synthesis of triacylglycerols (TAGs) were found, along with seven unigenes encoding PDCT (Unigene0000909), LPCAT (Unigene0007846), Oleosin3 (Unigene0010027), PDAT1 (Unigene0016056), GPDH (Unigene0022660), FAD2 (Unigene0037808) and FAD3 (Unigene0044238); these also proved to be up-regulated in the final stage of seed development.

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Section: Discussionmentioning

confidence: 99%

Transcriptome analyses reveals the dynamic nature of oil accumulation during seed development of Plukenetia volubilis L.

Guo

Yan

et al. 2020

Sci Rep

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“…Sesamin (see Fig 6 ), which exists in the oil of sesame seeds and some other plants, was one of the major components, as shown in Table 1 . It exhibits a variety of biological activities, such as lipid-lowering [ 13 ], antihypertensive [ 14 ], antioxidant [ 15 ], and anticancer effects. [ 16 ] In regard to its antitumor effects, Sesamin has already been confirmed to be active against several cancer cell types, including prostate cancer, breast cancer, colon cancer, and human lung cancer[ 17 – 19 ].…”

Section: Resultsmentioning

confidence: 99%

Sesamin from Cuscuta palaestina natural plant extracts: Directions for new prospective applications

Abu‐Lafi

Makhamra

Rayan³

et al. 2018

PLoS ONE

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The aim of this study is to disclose the potential bioactive components of Cuscuta palaestina, a native parasitic natural plant of flora palaestina and to open direction towards new prospective application. GC-MS analysis identified 18 components in the methanolic extract of C. palaestina for the first time. The most appealing among them are Sesamin and two other phytosterols (Campesterol and Stigmasterol), all of which are documented in the scientific literature for their anticancer activity. Quantitation of Sesamin extracted from C. palaestina by HPLC-PDA with the use of three organic solvents showed that the Sesamin content in the methanolic extract was the highest. Following the disclosure of Sesamin presence in C. palaestina, we raised the question of whether it is produced naturally in C. palaestina or acquired from the host plant. The quantitation of Sesamin in C. palaestina was performed while being with five different host plants, and was compared with the amount of Sesamin in C. palaestina grown alone. The findings reveal that Sesamin is an endogenous secondary metabolite in C. palaestina. Thus, further studies are required to prove if C. palaestina can be used as an alternative source of anticancer phytochemicals, mainly Sesamin, and if proteins in the Sesamin production pathway could be valid biological targets for the development of novel and selective pesticides for control/ eradication of C. palaestina and maybe some other Cuscuta species. As well, the findings from this study raise a big question of whether inferring Sesamin production in C. palaestina could reduce its attack ability to host plants.

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“…Vitamin E ( α -tocopherol) was used as the standard with the same concentrations as the test samples. A placebo solution with equal volume of methanol and DPPH was made and this solution mixture was incubated at room temperature for 30 min [13]. The antioxidant activity was quantified using the following equation: % Scavenging activity = ((Ab -As)/Ab) ×100, where Ab denotes the absorbance of the blank and As denotes the absorbance of the sample.…”

Section: Antioxidant Activitymentioning

confidence: 99%

GC-MS analysis of the methanolic extracts of Smilax china and Salix alba and their antioxidant activity

Qadir¹,

Aqil²,

Ali³

et al. 2020

Turk J Chem

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Smilax china L. (family Smilacaceae) and Salix alba L. (family Salicaceae) are plants that have been traditionally used to treat various ailments in Indian and Chinese medicine. A quantitative estimation of the methanolic extracts of these plants was performed by GC-MS analysis to obtain insight into its phytoconstituents responsible for therapeutic action. The antioxidant potential of the methanol extracts of Smilax china (MESC) and Salix alba (MESA) were assessedwith DPPH by using a UV spectrophotometer at a wavelength of 517 nm. The prevailing compounds found in MESC were lactam sugars including 2,5-dimethyl-2,4-dihydroxy-3(2H)-furanon (1.40%), 1,5-anhydro-6-deoxyhexo-2,3-diulose (4.33%), and alpha-methyl-1-sorboside (1.80%); the two alkaloids found were 1,4-methane-4,4a,5,6,7,8,9,9a-octahydro-10,10-dimethyl cyclohepta[d] pyridazine (0.87%) and 1,3,7-trimethyl-2,6-dioxopurine(0.54%); terpenes included deltacadinene (0.39%), terpineol, ( +) -cedrol (22.13%), 3-thujanol (0.77%), and 9,10-dehydro-cycloisolongifolene (0.34%); fatty acids included cis-vaccenic acid (4.98%) and telfairic acid (1.10%); esters included 1,2,3-propanetriol diacetate (7.56%), 7-hexadecenoic acid, methyl ester (1.77%), eicosanoic acid, and methyl ester (0.95%); and glycerol included 1,2,3-propanetriol (28.75%). The interesting compounds found in MESA were reducing sugars like D-allose (4.40%) and pyrogallol (10.48%), alkaloids like caffeine (63.49%), and esters like methyl octadecanoate (0.53%). Both fractions revealed considerable antioxidant activity. The reported existing phenolic compounds and terpenes are responsible for the antioxidant activity of the plant extracts.

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Solvent Extraction and GC-MS Analysis of Sesame Seeds for Determination of Bioactive Antioxidant Fatty Acid/Fatty Oil Components

Cited by 24 publications

References 10 publications

Transcriptome analyses reveals the dynamic nature of oil accumulation during seed development of Plukenetia volubilis L.

Transcriptome analyses reveals the dynamic nature of oil accumulation during seed development of Plukenetia volubilis L.

Sesamin from Cuscuta palaestina natural plant extracts: Directions for new prospective applications

GC-MS analysis of the methanolic extracts of Smilax china and Salix alba and their antioxidant activity

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