Soluble Nef antigen of HIV‐1 is cytotoxic for human CD4+ T cells

Fujii, Yoichi; Otake, Kaori; Tashiro, Masato; Adachi, Akio

doi:10.1016/0014-5793(96)00859-9

Cited by 126 publications

(176 citation statements)

References 20 publications

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“…100 ng/ml, could be present in tissue compartments such as lymph nodes where macrophages and infected lymphocytes tightly interact (67,68). The low amounts of exogenous Nef detected in the serum of HIV-infected subjects could also result from the presence of immune complexes Nef-antiNef as reported previously (48).…”

Section: Discussionsupporting

confidence: 64%

“…The amounts of exogenous Nef detected in the serum of HIV-infected subjects is around 10 ng/ml (48). At 10 ng/ml, we observed that recombinant Nef protein activates NF-kB, AP-1, and JNK (Figs.…”

Section: Discussionmentioning

confidence: 68%

“…Exogenous Nef protein has been detected in serum from HIV-infected subjects (48). However its significance in regard to HIV pathogenesis has not been studied.…”

Section: Discussionmentioning

confidence: 99%

“…Exogenous Nef protein is detected in the serum of HIVinfected subjects (48). Both antibodies and cytotoxic T lymphocytes (CTLs) directed against Nef have been found in a large proportion of infected individuals (49,50).…”

mentioning

confidence: 99%

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Exogenous Nef Protein Activates NF-κB, AP-1, and c-Jun N-Terminal Kinase and Stimulates HIV Transcription in Promonocytic Cells

Varin¹,

Manna²,

Quivy³

et al. 2003

Journal of Biological Chemistry

102

111

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 68%

“…Exogenous Nef protein has been detected in serum from HIV-infected subjects (48). However its significance in regard to HIV pathogenesis has not been studied.…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Exogenous Nef Protein Activates NF-κB, AP-1, and c-Jun N-Terminal Kinase and Stimulates HIV Transcription in Promonocytic Cells

Varin¹,

Manna²,

Quivy³

et al. 2003

Journal of Biological Chemistry

102

111

View full text Add to dashboard Cite

“…It was showed that majority of the nef protein was shown to be on the luminal side of these vesicles, although there was clearly nef protein on the vesicle surface as well. Nef has been shown to be secreted from infected cells and in one report nef was found to be secreted in association with small membrane-bound vesicles [19][20][21][22] . These identification was supported this study to analysis supernatant which may contains small membrane bounded vesicles contains nef protein.…”

Section: Purity Determination By Sds-page/western Blots Analysis Of Tmentioning

confidence: 99%

Stable Transfection Study for Cloning and Expression of Hiv-1 Nef Protein in Hek 293 Cells

2017

Rasayan J Chem

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Human immunodeficiency virus continues to be a major global populace health issue, there were approximately 34 million people living with HIV in 2011. However, the development of anti -viral has blunted the AIDS epidemic in the Western world but globally the epidemic has not been curtailed. Nef is one of these accessory genes that are only present within HIV and SIV genome and thought to play a in the progression to AIDS. The given its central purpose in HIV Pathogenesis, Nef considered as a potential anti-viral target for preventing or at least delaying Pathogenesis. The biologically active 27 kDa myristoylated Nef protein expressed from HEK 293 is a protein model to be used for significantly specific antibody production to lower the pathogenicity of HIV infection. Cultivated HEK 293 was transfected by 4ug/cubic centimetre of successful clone of pQBI-Nef-6His by stable transfection selected in 0.7 mg/cc of G418 antibiotic. It display that targeted 27 kDa HIV-1 Nef was not successfully expressed in HEK 293 cells either in stable transfection when transfected. However, non-targeted HIV-1 Nef was detected in western blot by anti-Nef (anti manufactured by Thermo scientific. It suggesting that, the Nef protein that was detected not identically synthesis through post-translation modification though it was expressed in the cytoplasm of HEK 293 cells. The ability of not expressing the targeted myristoylated 27 kDa nef protein was to various unpreventable factors due to time limitation and lacking of skills in the filed cloning and cell culture.

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Immunoregulatory effects of HIV‐1 Nef protein

et al. 2009

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During HIV infection, the perturbation of the adaptive and innate immune responses contributes to the progressive immunosuppression leading to an increased susceptibility to opportunistic infections and neoplastic diseases. Several impairments observed in HIV-infected patients include a gradual loss of CD4(+) T cells, CD8(+) T cell dysfunction, and a decreased number and function of natural killer (NK) cells. Moreover, a functional impairment and variation in the number of DC and B cells were observed during HIV infection. HIV-1 codes for proteins, including the accessory Nef proteins, that interacting with immune cells may contribute to AIDS pathogenesis. Here, we review the recent progress on the immunomodulatory effect of the accessory Nef protein and its role in the pathogenesis of HIV-1 infection. (c) 2009 International Union of Biochemistry and Molecular Biology, Inc.

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Soluble Nef antigen of HIV‐1 is cytotoxic for human CD4+ T cells

Cited by 126 publications

References 20 publications

Exogenous Nef Protein Activates NF-κB, AP-1, and c-Jun N-Terminal Kinase and Stimulates HIV Transcription in Promonocytic Cells

Exogenous Nef Protein Activates NF-κB, AP-1, and c-Jun N-Terminal Kinase and Stimulates HIV Transcription in Promonocytic Cells

Stable Transfection Study for Cloning and Expression of Hiv-1 Nef Protein in Hek 293 Cells

Immunoregulatory effects of HIV‐1 Nef protein

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