Solid state fermentation with recovery of Amyloglucosidase from extract by direct immobilization in cross linked enzyme aggregate for starch hydrolysis

Gupta, Kapish; Jana, Asim Kumar; Kumar, Sandeep; Jana, Mithu Maiti

doi:10.1016/j.bcab.2015.07.007

Cited by 20 publications

(8 citation statements)

References 46 publications

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“…An excellent growth of A. niger NRRL3 was achieved on soy hull pellets under SSF and an appropriate endoglucanase activity was obtained. Besides, SSF has the advantage of using agro-industrial waste for the production of enzymes at low cost, leading to high yields (Boggione et al, 2016;Gupta et al, 2015).…”

Section: Endoglucanase Precipitation From Fungal Extractmentioning

confidence: 99%

Single method of purification for endoglucanase from Aspergillus niger by polyelectrolyte precipitation

Boggione

Becher

Farruggia

2016

Biocatalysis and Agricultural Biotechnology

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The precipitation of endoglucanase from Aspergillus niger with synthetic and natural electrically charged polymers-poly vinyl sulfonate (PVS) and chitosan (CHS)-was characterized and applied to a simple method of purification of an enzymatic extract obtained from fungal culture under solid-state fermentation (SSF). The kinetics of complex formation was determined. The results of the kinetic profile obtained for CHS and PVS indicated an exothermic mechanism for the formation of the non-soluble complex. CHS exhibited a marked stabilizing effect on endoglucanase. The enzyme precipitated successfully with both polymers. The precipitation method applied to commercial endoglucanase and the fungal extract showed similar patterns with high purification factors. The recovery of the activity in the re-dissolved precipitate from the fungal extract was close to 40% at pH 5.3 using PVS (1% w/w) as precipitating agent and the purification factor was near 9. The purification factor of endoglucanase in the precipitate of the enzymatic extract from SSF with CHS (0.05% w/v) was around 7. These parameters make this precipitation method appropriate to be included in the last stages of a downstream process, with advantages such as simplicity, scalability and ability to concentrate and stabilize the enzyme.

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Section: Endoglucanase Precipitation From Fungal Extractmentioning

confidence: 99%

Single method of purification for endoglucanase from Aspergillus niger by polyelectrolyte precipitation

Boggione

Becher

Farruggia

2016

Biocatalysis and Agricultural Biotechnology

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“…In addition, these steps also incur extra downstream processing costs. Ideally, the enzyme purification and stabilization should be achieved simultaneously, and recently a few related techniques have been reported, such as support surface activation [16,21,22], protein-inorganic composite formation [23], and enzyme selfcrosslinking [24][25][26]. As discussed above, the functionalized TiO 2 nanoparticle can be a promising candidate for enzyme immobilization.…”

Section: Introductionmentioning

confidence: 99%

Direct immobilization of laccase on titania nanoparticles from crude enzyme extracts of P. ostreatus culture for micro-pollutant degradation

Nguyen

Hou

et al. 2017

Separation and Purification Technology

135

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“…In previous works the use of the proven technology of submerged fermentation has been widely reported (Meinicke et al, 2012;Prajapati et al, 2014;Rustiguel et al, 2015;Vendruscolo et al, 2013) due to better monitoring and ease of handling. However, SSF (Solid-state fermentation) offers an alternative for low-cost enzyme production and it has different advantages such as greater yields, lower investment costs and lower energy demand (Gupta et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Potential use of soybean hulls and waste paper as supports in SSF for cellulase production by Aspergillus niger

Boggione

Belén

Bassani

et al. 2016

Biocatalysis and Agricultural Biotechnology

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Cellulase has by vast applications in the biofuel, pulp and paper, detergent and textile industries. The three components of the enzyme complex (endoglucanase, exoglucanase and β-glucosidase) can effectively depolymerize the cellulose chains in lignocellulosic substrate. Solid-state fermentation (SSF) by fungi is a preferable production route for cellulase because of its low cost, among other advantages. This work describes the cellulase production by Aspergillus niger NRRL3 grown on SSF. SSF was carried out on soybean hulls and waste paper as supports. The effect of the support on cellulase production was assessed under a completely randomized factorial design. The support-time interaction was significant for all the variables studied. Both materials were characterized in terms of water absorption index and critical humidity point. Samples of culture were analyzed with scanning electron microscopy (SEM) to study spores and fungal growth. Maximum endoglucanase activity was found at 96 h using soybean hulls as support (5914.29 U L-1), being four times higher than that obtained using waste paper at the same fermentation time. The exoglucanase activity in soybean hulls was maximal at 96 h (4551.19 U L-1), being 9.6 times higher than that obtained in waste paper at the same time. The maximum β-glucosidase activity in soybean hulls (984.01 U L-1) was reached at 96 h, being 1.7 times greater than that obtained in waste paper. Besides, the use of soybean hulls provided high volumetric productivities at shorter times, which may decrease production costs considering a scaled process.

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Solid state fermentation with recovery of Amyloglucosidase from extract by direct immobilization in cross linked enzyme aggregate for starch hydrolysis

Cited by 20 publications

References 46 publications

Single method of purification for endoglucanase from Aspergillus niger by polyelectrolyte precipitation

Single method of purification for endoglucanase from Aspergillus niger by polyelectrolyte precipitation

Direct immobilization of laccase on titania nanoparticles from crude enzyme extracts of P. ostreatus culture for micro-pollutant degradation

Potential use of soybean hulls and waste paper as supports in SSF for cellulase production by Aspergillus niger

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