Solid‐phase microextraction, an alternative method for the study of airborne insect pheromones (Metamasius hemipterus, coleoptera, curculionidae)

Abstract

Short Coimnunicationslimits for the test amino acids were estimated to be in the range bctween 100 femtomoles (for leucine) and 300 femtomoles (for aspartic acid). These detection limits are approximately three orders of magnitude lower than those obtained in LC of amino acids with indirect CL detection 1141, but about two orders of magnitude higher than those found in CE of labeled amino acids using direct CL detection [5].The indirect CL detection scheme can be used for the determination of a wide variety of analytes provided that such analytes complex strongly and rapidly with Cu(I1). FiFure 3 shows the electropherogram of an opioid peptide, [D-ah ,D-leu 1-enkepha h , obtained with indirect CL detection. Other possible candidates include amines, catechol, catecholamines, and proteins. 4 ConclusionThe feasibility of using indirect CL detection in CE has been demonstrated. This detection mode is simpler than direct CL detection because pre-or postcapillary dcrivatization of non-CL analytes is not required. Detection is based on suppression of the Cu(1l) catalysis of the luminol CL reaction. Work is now in progress to investigate the optimization of the overall system in order to enhance the detection sensitivity for the determination of protein.

Cuticular compounds of workers and queens in two Leptothorax ant species — a comparison of results obtained by solvent extraction, solid sampling, and SPME

“…c) Solid-Phase-Microextraction (SPME). Cuticular substances of individual workers were sampled with two different types of Supelco polydimethylsiloxane (PDMS) fibers (Berlardi & Pawliszyn 1989;Arthur & Pawliszyn 1990;Arthur et al 1992;Zhang & Pawliszyn 1993;Malosse et al 1995;Frèrot et al 1997;Monnin et al 1998): a fiber with a 7 mm layer designed to extract compounds of high molecular weight, and a 30 mm fiber suitable for the extraction of non-polar semivolatiles. The fiber was carefully rubbed against the two last tergites of the abdomen of one individual live ant for 15 min, then desorbed in the injection port of a gas chromatograph for 5 min (splitless mode).…”

Headspace solid-phase microextraction (HS-SPME): a microscale sampling technique for determination of monoterpene hydrocarbons in coniferous needles by gas chromatography/mass spectrometry (GC/MS)

“…They are subsequently thermally desorbed from the fibre in the hot injector block of a gas chromatograph [3]. SPME has become very popular in the recent years, especially in environmental analysis [16,17,18,19,20,21], flavour and fragrance analyses [22,23], identification of insect pheromones [24], and the analyses of plant volatiles, including those from conifer needles [3]. The application of SPME can reduce the limitations associated with the current methodologies [22,25,26].…”

Quantitative Analysis of aliphatic aldehydes by headspace SPME sampling and ion-trap GC-MS

“…Because the method is fast, economic, and simple to use it can be used as an alternative to conventional headspace analysis and solvent extraction. SPME has already been used widely in environmental [3,4], biochemical [5,6], and food and beverage [7,8] analysis. The principle of SPME is an equilibrium partitioning of the analyte between the coating of a silica fiber and sample solution or headspace.…”