Solicitation of HPLC and HPTLC Techniques for Determination of Rutin from Polyalthia longifolia Thwaites

Doshi, Gaurav Mahesh; Zine, Sandip; Chaskar, Pratip K.; Une, Hemant D.

doi:10.4103/0974-8490.132601

Cited by 13 publications

(11 citation statements)

References 5 publications

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“…The standard and extract samples were dissolved in the methanol (10mg in 1ml) and were filtered through Whatman filter paper no.1 before spotting on the plates, exposed to ammonia and visualized under UV lamp. 9,10 HPTLC Standard (7mg/7ml of rutin and 5mg/5ml of quercetin) and fruit extracts (200mg/5ml) were dissolved in methanol. Quantification was done by application of the spots on HPTLC plate which were coated with silica gel (plate size of 20.0 x 10.0cm).…”

Section: Inmentioning

confidence: 99%

Quantification of rutin and quercetin by HPTLC/HPLC and in vitro immunomodulatory and anticancer activities of Capparis moonii fruits extracts

Doshi

Pawar

Chavda

2017

Int J Basic Clin Pharmacol

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Background: The current research was undertaken on dried fruits of Capparis moonii to screen its potential for immunomodulatory and cancer indications with identification of phytoconstituents by chromatographic techniques.Methods: Methanolic (MECN), hydro-methanolic (HMECN) and aqueous extracts (AQCN) of Capparis moonii were subjected to high performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC) after studying the total phenolic and flavonoid content by using rutin and gallic acid as standards respectively as well as undertaking powder characteristics and preliminary phytochemical screening. Immunomodulatory activities covered were hemagglutination antibody titre and delayed-type hypersensitivity reaction with the aid of sheep red blood cells (0.5×109) as antigens. The extracts were studied for antioxidant potential. Anticancer prospects were focusing on in vitro cell lines screening (MCF 7 and HCT 15) by Sulforhodamine B assay method and potato disc assay.Results: The total phenolic and flavonoid content of MECM, HMECM and AQCM fruits extracts were found to be 0.20, 0.11 and 0.47 mg of gallic acid/g and 78.3, 18.8 and 64.4 mg of rutin/g respectively. Rutin and quercetin were confirmed by HPTLC and HPLC showing well resolved peaks. IC50 values in antioxidant studies were found to be significant with all the extracts. Significant immunomodulatory effect was noticed at 200mg/kg in both models (high antibody titre levels and decrease paw volume after 48 h). Unsatisfactory results were observed with selected cell lines and disc assay.Conclusions: Thus, selected fruits may probably have immunomodulatory potential due to presence of flavonols (rutin and quercetin).

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Section: Inmentioning

confidence: 99%

Quantification of rutin and quercetin by HPTLC/HPLC and in vitro immunomodulatory and anticancer activities of Capparis moonii fruits extracts

Doshi

Pawar

Chavda

2017

Int J Basic Clin Pharmacol

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“…[18] • Rutin: Mobile phase used was methanol:glacial acetic acid:formic acid:water (3:0.9:0.9:0.5) • Quercetin: Mobile phase used was methanol:glacial acetic acid:formic acid:water (3:0.9:0.9:0.5).…”

Section: Part B: Analytical Studiesmentioning

confidence: 99%

Untitled

Doshi

2016

IJGP

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Background: Herbal therapy is eventually based on the knowledge and practitioner skills. In India, local plants, as well as shrubs, can be procured easily due to influence of ancient Ayurveda. Taking into due considerations, we investigated Cestrum nocturnum (CN) Linn. commonly known as Raat rani. The shrub has been reported to have a wide arena of ethnopharmacological relevance. Objective: In the current research studies, our aim was to develop controlled release herbal tablets from the CN extract followed by in vitro screening on colon cancer cell line and correlate the quantified phytoconstituents such as rutin and quercetin by high performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC). Materials and Methods: The leaves were macerated in methanol. The extract after preliminary phytochemical screening was subjected to sophisticated methods such as HPTLC and HPLC to elucidate rutin and quercetin which were found to positive by thin layer chromatography. To develop herbal tablets, the extract was subjected to excipient profile compatibility which was confirmed by Fourier transmission infrared (FTIR) spectrometry. Wet granulation method was used to develop the formulation. The developed herbal tablets were screened for colon cancer targeted cell line (HCT 15) using sulforhodamine B assay method with four dose levels of different concentrations. The results were analyzed as a percentage of growth inhibition 50%. Results: HPTLC confirmed the presence of spots of rutin (4.15% w/w) and quercetin (10.61% w/w) in comparison to the standard at R f value 0.47 and 0.29, respectively. HPLC chromatogram showed the presence of rutin and quercetin with retention time 5.391 min and 6.698 min, respectively, in comparison to standard at 360 nm. The FTIR revealed no interaction of drug and excipients since the peaks of both are observed to be identical. Unsatisfactory results were obtained for the formulation on the selected cell line. Conclusion: In future, the world needs to explore, screen and develop herbal formulations which can be found to be compatible for cancer chemotherapy.

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“…[24][25][26] The procedure comprises of Methanol: Phosphate Buffer (pH 3) as mobile phase in ratio 70:30. 2 mg of standard and 5 mg of the sample were dissolved in 2 ml and 5 ml of solvent respectively.…”

Section: Methodsmentioning

confidence: 99%

Revelation of β-sitosterol from Benincasa hispida Seeds, Carissa congesta Roots and Polyalthia longifolia Leaves by High Performance Liquid Chromatography

Doshi¹,

Chaskar²,

Une³

2016

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Background: Fruit juice of Benincasa hispida (BH) is regarded as Vrindamadhava that is recommended for internal use in snake's bites. The fruits have been reported to contain good amount of proteins, enzymes, Vitamin B 1 and C, flavonoid C-glycoside, terpenes, phenolic acids and free sugars such as glucose, rhamnose, mannitol, uronic acid, astilbin, catechin, naringenin, pectic polysaccharides and even some trace metals. Polyalthia longifolia (PL) has been well known for its ayurvedic preparations such as Sitaphala kula, Kaphapitashama, Anulomak and Krimighna. PL leaves has been reported to contain phytoconstituents like polylongine, Aporphine-Noxide alkaloids, allo-aromadendrene, caryophyllene oxide, β-caryophyllene, β-selinene, α-humulene, α-pinene and camphene. Carissa congesta (CC), known as Bengal currant, have yielded 2-acetyl phenol, carissone, carindone and Des-n-methylnoracronycine. β-sitosterol, an important phytoconstituent recognized from all these selected plants has been well known for its ethnopharmacological importance. In this new study, the research team members has focused on determining the percentage of the β-sitosterol present in the BH seeds, CC roots and PL leaves by subjecting the ex-

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Solicitation of HPLC and HPTLC Techniques for Determination of Rutin from Polyalthia longifolia Thwaites

Cited by 13 publications

References 5 publications

Quantification of rutin and quercetin by HPTLC/HPLC and in vitro immunomodulatory and anticancer activities of Capparis moonii fruits extracts

Quantification of rutin and quercetin by HPTLC/HPLC and in vitro immunomodulatory and anticancer activities of Capparis moonii fruits extracts

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Revelation of β-sitosterol from Benincasa hispida Seeds, Carissa congesta Roots and Polyalthia longifolia Leaves by High Performance Liquid Chromatography

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