Solamargine: The Main Glycoalkaloid from the Fruits of<i>Solanum paludosum</i>

Valverde, Michèle; Lavaud, Catherine; Boustié, Joël; Badaoui, H. El; Muguet, Bruno; Henry, Max

doi:10.1055/s-2006-959743

Cited by 13 publications

(11 citation statements)

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“…The extraction and the purification of solamargine were described previously (Valverde et al, 1993). The method was derived from Cham & Wilson (1987).…”

Section: Extraction Purification and Quantitative Determination Of Smentioning

confidence: 99%

“…Previously, Valverde et al (1993) reported the presence of about 3% of solamargine, based on the dry weight, in the unripe fruits of a north-eastern brasilian shrub, Solanum paludosum Moric. The presence of steroidal alkaloids was established from a work performed on unripe fruits of S. paludosum by identification of solasodine, the aglykone obtained by hydrolysis of the crude extract and not directly from the glycoalkaloids (Battacharyya, 1984).…”

Section: Introductionmentioning

confidence: 99%

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Stimulation of the growth and solamargine production by Solanum paludosum multiple shoot cultures using a new culture medium

Badaoui

Morard

Henry

1996

Plant Cell Tiss Organ Cult

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Organogenic callus cultures of Solanum paludosum were obtained from root, hypocotyle and cotyledon explants of plantlets cultured in sterile conditions. These callus cultures developed multiple shoots which proliferated in Murashige and Skoog basal liquid medium. These multiple shoots produced solamargine, the main steroidal glycoalkaloid present in the unripe fruits.The optimization of the macronutrient composition of the liquid medium was performed by a method derived from the plant composition. This approach results in the establishment of an appropriate medium (SPOM medium) suitable for the improvement of both growth and solamargine production by multiple shoot cultures ofS. paludosum.

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“…The extraction and the purification of solamargine were described previously (Valverde et al, 1993). The method was derived from Cham & Wilson (1987).…”

Section: Extraction Purification and Quantitative Determination Of Smentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Stimulation of the growth and solamargine production by Solanum paludosum multiple shoot cultures using a new culture medium

Badaoui

Morard

Henry

1996

Plant Cell Tiss Organ Cult

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“…The plates were heated at 120°C for 10 min and were read on a Vernon densitometer at 420 nm. Solamargine was conclusively detected by comparison of its migration with that of an authentic sample prepared previously (Valverde et al, 1993) and run under identical conditions. The determination of solarmargine was achieved by comparison with a calibration graph.…”

Section: Chemical Analysismentioning

confidence: 94%

“…The extraction and the purification of solamargine has been described previously (Valverde et al, 1993). The method was derived from Cham & Wilson (1987).…”

Section: Chemical Analysismentioning

confidence: 99%

“…Recently, Valverde et al (1993) reported the presence of about 2.5 to 3% solamargine (dry weight) in the unripe fruits of a north-eastern brasilian shrub, S. paludosum Moric. The presence of steroidal alkaloids was established in work (Battacharyya et al, 1984) performed also on unripe fruits of S. paludosum, but this was estimated from the aglykone, solasodine, after hydrolysis of the crude extract, and not directly from the glycoalkaloids.…”

Section: Introductionmentioning

confidence: 99%

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Production of solamargine by in vitro cultures of Solanum paludosum

Badaoui

Muguet

Henry

1996

Plant Cell Tiss Organ Cult

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Callus cultures of Solanum paludosum were established from roots, hypocotyles, cotyledons and leaf limbs of plantlets cultivated in sterile conditions on a Murashige and Skoog's modified medium. Non organogenous calluses were obtained with addition of BA or kinetin (10-SM to 10-6M) as the cytokinin and 2,4-D or NAA (10-SM to 10-6M) as the auxin. These calluses permitted the establishment of a cell suspension culture with BA (10-6M) and 2,4-9 (10-6M). Zeatin (10-6M) with IAA (10-6M) gave rise to organogenous calluses. These organogenous callus cultures developed multiple shoots which either proliferated if they were cultivated on a medium containing zeatin with IAA or IBA or were able to regenerate into whole plants when zeatin was used as the only hormone. The different plant material produced solamargine, the main steroidal glycoalkaloid present in the unripe fruits. The best production was obtained with the fruits of regenerated plants from organogenous callus cultures after reintroduction of these plants in their brasilian biotope. The solamargine content of the two types of plant materials was about 0.06% and 2.5% (dry weight) respectively for the callus cultures and the fruits from in vitro plants. The fruits were harvested a year after the beginning of the plantlet regeneration step.Abbreviations: HPTLC -high performance thin layer chromatography; HPLC -high performance liquid chromatography; 2,4-D -2,4-dichlorophenoxyacetic acid; BA -benzylaminopurine; IAA -3-indolebutyric acid; NAA -a-naphthaleneacetic acid; IBA -3-indolebutyric acid; IPA -isopentenyladenine

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The Genus Solanum: An Ethnopharmacological, Phytochemical and Biological Properties Review

Kaunda

Zhang

2019

Nat. Prod. Bioprospect.

103

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Over the past 30 years, the genus Solanum has received considerable attention in chemical and biological studies. Solanum is the largest genus in the family Solanaceae, comprising of about 2000 species distributed in the subtropical and tropical regions of Africa, Australia, and parts of Asia, e.g., China, India and Japan. Many of them are economically significant species. Previous phytochemical investigations on Solanum species led to the identification of steroidal saponins, steroidal alkaloids, terpenes, flavonoids, lignans, sterols, phenolic comopunds, coumarins, amongst other compounds. Many species belonging to this genus present huge range of pharmacological activities such as cytotoxicity to different tumors as breast cancer (4T1 and EMT), colorectal cancer (HCT116, HT29, and SW480), and prostate cancer (DU145) cell lines. The biological activities have been attributed to a number of steroidal saponins, steroidal alkaloids and phenols. This review features 65 phytochemically studied species of Solanum between 1990 and 2018, fetched from SciFinder, Pubmed, ScienceDirect, Wikipedia and Baidu, using “Solanum” and the species’ names as search terms (“all fields”).

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Solamargine: The Main Glycoalkaloid from the Fruits ofSolanum paludosum

Cited by 13 publications

References 1 publication

Stimulation of the growth and solamargine production by Solanum paludosum multiple shoot cultures using a new culture medium

Stimulation of the growth and solamargine production by Solanum paludosum multiple shoot cultures using a new culture medium

Production of solamargine by in vitro cultures of Solanum paludosum

The Genus Solanum: An Ethnopharmacological, Phytochemical and Biological Properties Review

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