Sodium dodecyl sulphate electrophoresis of wheat gliadins

Ewart, J. A. D.

doi:10.1002/jsfa.2740240608

Cited by 21 publications

(6 citation statements)

References 18 publications

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“…This range was in agreement with values (44K-78K) reported by Tatham and Shewry (1990). In addition, Ewart (1973) estimated the polypeptide chain weights of R-, β-, and γ-gliadin fractions that were in the range of 32K-44K. Spring spelt accessions SK0505, SK0263, and RL5407 had identical protein subunit patterns based on SDS-PAGE (Figure 7A).…”

Section: Resultsmentioning

confidence: 99%

Electrophoretic Characterization of Spring Spelt Wheat Gliadins

Abdel-Aal

Salama

Hucl

et al. 1996

J. Agric. Food Chem.

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Gliadin compositions of 95 spring spelts, 5 winter spelts, and Neepawa Hard Red Spring (HRS) wheat were evaluated by acid−polyacrylamide gel electrophoresis (A-PAGE). Five spring spelts grown in three environments over 2 years were compared to the HRS cultivar Katepwa using A-PAGE and sodium dodecyl sulfate−polyacrylamide gel electrophoresis (SDS-PAGE). On the basis of the scoring of 13 bands, the spring and winter spelts diverged widely from Neepawa HRS wheat. None of the spring spelts evaluated had a gliadin protein profile identical to that of commercially available winter spelt. Spring and winter spelts were differentiated from Katepwa HRS wheat based on ω-gliadin composition, in which common wheat was characterized by the presence of a slow-moving ω-gliadin and a strong-staining fast-moving ω-gliadin. Common wheat and spring spelt accessions SK0505, SK0263, and RL5407 (black-hulled spelts) had a distinct strong band in the γ-gliadin region with MW of about 64K which was absent in winter spelts and the spring spelts SK0021 and PGR8801 (white-hulled spelts). The molecular weights of gliadin proteins were in the range of 34K−75K. Keywords: Spelt; wheat; gliadin; electrophoresis; A-PAGE; SDS-PAGE, NTSYS-PC

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Section: Resultsmentioning

confidence: 99%

Electrophoretic Characterization of Spring Spelt Wheat Gliadins

Abdel-Aal

Salama

Hucl

et al. 1996

J. Agric. Food Chem.

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“…Zones c, d and e of Fig. 6 correspond to gliadins (MW between 30,000 and 45,000) (Bietz and Wall 1972;Sexson and Wu 1972;Ewart 1973). The relative area of these zones increased in the first day of germination in the four lots of seeds (Fig.…”

Section: Resultsmentioning

confidence: 95%

DRYING AND NaF ALTER VIABILITY AND SOLUBLE PROTEINS OF GERMINATING WHEAT SEEDS

Lupano

Añón

1990

J Food Biochemistry

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The effect of heat drying and NaF on the viabili9 and soluble proteins of wheat seeds was analyzed. Protein content measurements and sodium dodecyl sulfate polyacrylamide gel electrophoresis were carried out on germ (plantlet) and endosperm during the first four days of germination. Results showed that the viability of both normal and heated seeds decreased exponentially with NaF concentration. The parameter most affected by both NaF and heating was plantlet size. Although some differences in the electrophoretic patterns corresponding to different treatments were found, results showed similar trends in most of them, and the main changes appeared between different days of germination. Additive effects of both NaF and heating were observed in some cases.

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“…Sodium dodecyl sulfate has been very widely used to solubilize proteins. Examples include mycoplasma protein (49), wheat gliadins (65), cell protein membranes (66), erythrocyte membranes (67), proteins of retina photoreceptors (213), cell membrane proteins (165), glomerular basement membranes substances (168), polypeptides from zymogen granule membranes (151), ribonucleic acids (99), and envelope proteins from Escherchi coli (108). The nonionic detergent Triton X-100 is useful for solubilizing membrane proteins prior to their identification by Immunoelectrophoresis (52).…”

Section: Conventional Electrophoresismentioning

confidence: 99%

Electrophoresis

Strickland¹

1974

Anal. Chem.

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This review extends the coverage of a previous one (227) by citing articles published between the latter half of 1969 and the end of 1973. The areas of discussion will be biased toward significant developments in methodology and theory. Applications are mentioned only for their illustrative value. Where possible, this deficiency is compensated for by references to books or reviews.METHODS AND APPARATUS Particle Electrophoresis. Continuous free curtain electrophoresis has been used to separate 16 classes of lymphoid cells (275), subcellular particles (197), and microsomes (113). Both the methodology (98) and the limitations (266) of the method have been reviewed. Factors af-

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Sodium dodecyl sulphate electrophoresis of wheat gliadins

Cited by 21 publications

References 18 publications

Electrophoretic Characterization of Spring Spelt Wheat Gliadins

Electrophoretic Characterization of Spring Spelt Wheat Gliadins

DRYING AND NaF ALTER VIABILITY AND SOLUBLE PROTEINS OF GERMINATING WHEAT SEEDS

Electrophoresis

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