Electrophoresis

Abstract

This review extends the coverage of a previous one (227) by citing articles published between the latter half of 1969 and the end of 1973. The areas of discussion will be biased toward significant developments in methodology and theory. Applications are mentioned only for their illustrative value. Where possible, this deficiency is compensated for by references to books or reviews.METHODS AND APPARATUS Particle Electrophoresis. Continuous free curtain electrophoresis has been used to separate 16 classes of lymphoid cells (275), subcellular particles (197), and microsomes (113). Both the methodology (98) and the limitations (266) of the method have been reviewed. Factors af-

Electrophoresis

“…Neither the phosphonic nor the sulfonic acid groups offered advantages over the carboxylates of Ampholine, and the new ampholyte tended to form complexes both with itself and with proteins (109). Several papers cited in the previous review (137) suggested that Ampholine could form complexes with proteins, but it has been shown that this does not occur with albumin, ferritin, ßglucuronidase, or serum proteins (34) or with human growth hormone or ovine prolactin (8). Recently, Ampholine has contained materials that are stained by the commonly used dyes.…”