Snapshot imprinting: rapid identification of cancer cell surface proteins and epitopes using molecularly imprinted polymers

Piletsky, Stanislav S.; Piletska, Elena; Poblocka, Marta; Macip, Salvador; Jones, Donald J. L.; Braga, Marta; Cao, Thong Huy; Singh, Randhir; Spivey, Alan C.; Aboagye, Eric O.; Piletsky, Sergey A.

doi:10.1016/j.nantod.2021.101304

Cited by 30 publications

(24 citation statements)

References 39 publications

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“… 18 The polymerisation of nanoparticles was performed in relatively mild conditions that did not cause cell lysis. 19 …”

Section: Resultsmentioning

confidence: 99%

“…The epitopes sequences were analysed providing information about types of the proteins exposed on cell membranes, and which contributed to the formation of nanoMIPs, and their abundance. 19 …”

Section: Resultsmentioning

confidence: 99%

“…18 The polymerisation of nanoparticles was performed in relatively mild conditions that did not cause cell lysis. 19 In theory, since monomers and initiators cannot penetrate inside cells during the short time of the polymerisation reaction, only epitopes of the proteins exposed on the cell surface contributed to the formation of nanoMIPs. Trypsin was used to digest the proteins, releasing from the cells nanoMIPs with fragments of proteins protected by their interactions with the polymeric nanoparticle.…”

Section: Resultsmentioning

confidence: 99%

“…The NCI-H23, NCI-H460, NCI-H522 and A549 cell lines were analysed individually as described earlier. 19 A monomeric mixture was prepared by dissolving 19.5 mg N -isopropylacrylamide (NIPAm), 3 mg N , N ′-methylene-bis-acrylamide (MBAA), 15 mg N-tert -butylacrylamide (TBAm), and 3 mg 3-aminopropylmethacrylate in 50 mL of PBS and purged with nitrogen for 20 min. The growth media was removed from 250 mL cell culture flasks containing lung cancer cells and cells washed 4 times with 4 × 20 mL of PBS.…”

Section: Methodsmentioning

confidence: 99%

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Molecular imprinting as a tool for determining molecular markers: a lung cancer case

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“… 18 The polymerisation of nanoparticles was performed in relatively mild conditions that did not cause cell lysis. 19 …”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Molecular imprinting as a tool for determining molecular markers: a lung cancer case

et al. 2022

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“…27 This latter approach has proved particularly useful for obtaining high affinity protein-imprinted nanopolymers, characterized by high selectivity for the template and complete compatibility with aqueous environments. [28][29][30][31][32][33][34][35][36] Moreover, as the template is covalently grafted onto the solid phase, the isolation and purification of nanoMIPs is an easy task, and no residual protein remains trapped in the nanoparticles, avoiding product contamination.…”

Section: Introductionmentioning

confidence: 99%

Rabbit IgG-imprinted nanoMIPs by solid phase synthesis: the effect of cross-linkers on their affinity and selectivity

et al. 2022

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A High‐Throughput Screening Strategy for Synthesizing Molecularly Imprinted Polymer Nanoparticles Selectively Targeting Tumors

Song,

Li,

et al. 2024

Adv Healthcare Materials

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Molecularly imprinted polymers (MIPs) show significant promise as effective alternatives to antibodies in disease diagnosis and therapy. However, the challenging process of screening extensive libraries of monomer combinations and synthesis conditions to identify formulations with enhanced selectivity and affinity presents a notable time constraint. The need for expedient methods becomes clear in accelerating the strategic development of MIPs tailored for precise molecular recognition purposes. In this study, an innovative high‐throughput screening methodology designed to identify the optimal MIP formulation for targeting tumors is presented. Employing a microtiter plate format, over 100 polymer syntheses are conducted, incorporating diverse combinations of functional monomers. Evaluation of binding performance utilizes fluorescence‐based assays, focusing on an epitope of the epidermal growth factor receptor (EGFR). Through this meticulously structured screening process, synthesis conditions that produced MIP nanoparticles exhibiting substantial specificity for EGFR targeting (KD = 10−12 m) are identified. These “bionic antibodies” demonstrate selective recognition of cancer cells in whole blood samples, even at concentrations as low as 5 cells mL−1. Further validation through fluorescent imaging confirms the tumor‐specific localization of the MIPs in vivo. This highly efficient screening approach facilitates the strategic synthesis of imprinted polymers functioning as precision bioprobes.

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Snapshot imprinting: rapid identification of cancer cell surface proteins and epitopes using molecularly imprinted polymers

Cited by 30 publications

References 39 publications

Molecular imprinting as a tool for determining molecular markers: a lung cancer case

Molecular imprinting as a tool for determining molecular markers: a lung cancer case

Rabbit IgG-imprinted nanoMIPs by solid phase synthesis: the effect of cross-linkers on their affinity and selectivity

A High‐Throughput Screening Strategy for Synthesizing Molecularly Imprinted Polymer Nanoparticles Selectively Targeting Tumors

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