Smooth Muscle Cells Differentiated From Reprogrammed Embryonic Lung Fibroblasts Through DKK3 Signaling Are Potent for Tissue Engineering of Vascular Grafts

Karamariti, Eirini; Margariti, Andriana; Winkler, Bernhard; Wang, Xiaocong; Hong, Xuechong; Baban, Dilair; Ragoussis, Jiannis; Huang, Yi; Han, Jing–Dong Jackie; Wong, Man Sau; Sag, Can Martin; Shah, Ajay M.; Hu, Yanhua; Xu, Qingbo

doi:10.1161/circresaha.111.300415

Cited by 86 publications

(104 citation statements)

References 41 publications

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“…Since 2000, several transcription factors were discovered and were successfully used to reprogram target cells such as pancreatic islet cells (Zhou et al, 2008), neurons (Fishman et al, 2015;Vierbuchen et al, 2010), hepatocytes (Huang et al, 2011;Sekiya and Suzuki, 2011), endothelial cells (Ginsberg et al, 2012;Han et al, 2014), smooth muscle cells (Karamariti et al, 2013), and hepatocyte like cells (Simeonov and Uppal, 2014).…”

Section: Direct Reprogrammingmentioning

confidence: 99%

Direct reprogramming of somatic cells: an update

Pham

2015

Biomed Res Ther

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Abstract-Direct epigenetic reprogramming is a technique that converts a differentiated adult cell into another differentiated cell-such fibroblasts to cardiomyocytes-without passage through an undifferentiated pluripotent stage. This novel technology is opening doors in biological research and regenerative medicine. Some preliminary studies about direct reprogramming started in the 1980s when differentiated adult cells could be converted into other differentiated cells by overexpressing transcription-factor genes. These studies also showed that differentiated cells have plasticity. Direct reprogramming can be a powerful tool in biological research and regenerative medicine, especially the new frontier of personalized medicine. This review aims to summarize all direct reprogramming studies of somatic cells by master control genes as well as potential applications of these techniques in research and treatment of selected human diseases.

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Section: Direct Reprogrammingmentioning

confidence: 99%

Direct reprogramming of somatic cells: an update

Pham

2015

Biomed Res Ther

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“…More recently, stem cells from various sources have been exploited for vascular tissue engineering applications. Indeed, a variety of stem cell types including embryonic stem cells 7 , induced pluripotent stem cells (iPSCs) 8,9 , PiPSC 10,11 , bone marrow-derived mononuclear cells 12 , mesenchymal stem cells 13 , endothelial progenitor cells and adult vessel wallderived stem cell antigen-1 (Sca-1)+ stem/progenitor cells 14,15 have all been demonstrated to be capable of differentiation into either functional endothelial or smooth muscle cells in response to defined media and culture conditions. Furthermore, the unlimited self renewal capacity of the stem cells make them better candidates unlike mature endothelial and smooth muscle cells which can only divide for a finite number of times before undergoing growth arrest and senescence.…”

Section: Introductionmentioning

confidence: 99%

“…To date, the most common materials used for scaffolds in vascular tissue engineering include polymers of polyglycolic acid, polylactic acid, and poly ε-caprolactone . 10,11 . Thereafter, we designed a decellularized vessel for seeding of PiPSC-derived vascular cells to closely mimic the matrix proteins that exists within a native vessel, thus enhancing grafting and survival efficacy.…”

Section: Introductionmentioning

confidence: 99%

“…Subsequent analysis showed that while mice grafted with decellularized vessels presented with markedly higher mortality rates as early as day 1, PiPSC vessel grafts conferred a survival rate of 60% 3 weeks after transplantation ( Figure 5A) 11 . Additionally, 3 week old PiPSC-derived grafts were fully characterized for the presence of human PiPSC-derived endothelial and smooth muscle cells and host macrophage infiltration ( Figure 5B and Table 1) …”

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confidence: 98%

“…Original magnification for images shown are 400X unless stated. This figure has been modified from Karamariti, E. et al 11 Please click here to view a larger version of this figure.…”

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confidence: 99%

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Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Wong

Hong

Karamariti

et al. 2015

JoVE

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The construction of vascular conduits is a fundamental strategy for surgical repair of damaged and injured vessels resulting from cardiovascular diseases. The current protocol presents an efficient and reproducible strategy in which functional tissue engineered vessel grafts can be generated using partially induced pluripotent stem cell (PiPSC) from human fibroblasts. We designed a decellularized vessel scaffold bioreactor, which closely mimics the matrix protein structure and blood flow that exists within a native vessel, for seeding of PiPSC-endothelial cells or smooth muscle cells prior to grafting into mice. This approach was demonstrated to be advantageous because immune-deficient mice engrafted with the PiPSC-derived grafts presented with markedly increased survival rate 3 weeks after surgery. This protocol represents a valuable tool for regenerative medicine, tissue engineering and potentially patient-specific cell-therapy in the near future. Video LinkThe video component of this article can be found at

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Challenges and Strategies for Endothelializing Decellularized Small‐Diameter Tissue‐Engineered Vessel Grafts

Ding,

Hou,

Gao

et al. 2024

Adv Healthcare Materials

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Vascular diseases are the leading cause of ischemic necrosis in tissues and organs, necessitating using vascular grafts to restore blood supply. Currently, small vessels for coronary artery bypass grafts are unavailable in clinical settings. Decellularized small‐diameter tissue‐engineered vessel grafts (SD‐TEVGs) hold significant potential. However, they face challenges, as simple implantation of decellularized SD‐TEVGs in animals leads to thrombosis and calcification due to incomplete endothelialization. Consequently, research and development focus has shifted toward enhancing the endothelialization process of decellularized SD‐TEVGs. This paper reviews preclinical studies involving decellularized SD‐TEVGs, highlighting different strategies and their advantages and disadvantages for achieving rapid endothelialization of these vascular grafts. We analyzed methods to improve the process while addressing potential shortcomings. This paper aims to contribute to the future commercial viability of decellularized SD‐TEVGs.This article is protected by copyright. All rights reserved

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Smooth Muscle Cells Differentiated From Reprogrammed Embryonic Lung Fibroblasts Through DKK3 Signaling Are Potent for Tissue Engineering of Vascular Grafts

Abstract: Conclusions:We developed a protocol to generate SMCs from PiPS cells through a dickkopf 3 signaling pathway, useful for generating tissue-engineered vessels. These findings provide a new insight into the mechanisms of SMC differentiation with vast therapeutic potential.

Cited by 86 publications

References 41 publications

Direct reprogramming of somatic cells: an update

Direct reprogramming of somatic cells: an update

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Challenges and Strategies for Endothelializing Decellularized Small‐Diameter Tissue‐Engineered Vessel Grafts

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