Smooth muscle associated antigen in astrocytes and astrocytomata

Abstract

Summary.-Four human and 4 rat astrocytomata and mammalian adult brain and spinal cord were examined by indirect immunofluorescence with human serum containing smooth muscle antibody. Cryostat sections of astrocytomata showed staining of the tumour cell cytoplasm and processes while in normal adult brain and spinal cord the entire astrocyte stained. Impression film and tissue culture monolayers of astrocytomata showed staining of cell processes and a fine, filamentous network in the cell body. The reaction with astrocytoma tumour cells was stronger than that with the corresponding normal astrocytes. Specificity of the staining reaction was established by its prevention on neutralization absorptions of the serum with -extracts or homogeneous of smooth muscle. The presence of smooth muscle-associated antigen in astrocytes and astrocytomata is indicative of contractile protein providing a mechanism of cell movement in vivo.

Increased expression of actin-like contractile protein in preneoplastic and neoplastic lesions in rat liver

“…We have previously shown that SMA serum also reacts with leukaemic cells (Toh, Muller and Cauchi, 1976c) and with experimental skin Toh and Muller, 1975) glial (Toh, Muller and Elrick 1976a) and renal mesenchymal tumours (Toh et al, 1976b); the reaction with the solid tumours is more intense, than that observed with the corresponding normal tissue. Similar observations have been made by Gabbiani, Trenchev and Holborow (1975) with human skin and breast tumours.…”

Léiomyomes multiples traités par nifédipine

“…Cependant, Toh et al [14] montraient en 1976 la présence d'un antigè ne associé au muscle lisse, plus abondant dans les astrocytomes que dans les astrocytes normaux. Harmer et al [7] émettaient par contre en 1983 l'hypothèse d'une augmenta tion non quantitative mais qualitative de l'actine, qui se présenterait alors sous une forme plus polymérisée dans certaines cellu les malignes.…”

Ultrastructure and Actin Distribution in Neoplastic Neurogenic Cells in Culture

“…A characteristic feature is that they often assemble into bundles, which can be visualized by antibodies against actin. myosin, or tropomyosin (Cabbiani,et al 1973, Toh et al 1976, Botazzo et af. 1976).…”