Increased expression of actin-like contractile protein in preneoplastic and neoplastic lesions in rat liver

Toh, Ban‐Hock; Cauchi, Maurice N.; Cook, Peter C.; Muller, HK

doi:10.1038/bjc.1977.117

Cited by 6 publications

(2 citation statements)

References 14 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The changes in actin isoform levels in cells are often connected with pathological processes. The changed levels of β- and γ-actins were shown to accompany many tumor types such as chemically induced melanoma, hepatoma, lymphoma and breast cancer (Nowak and Malicka-Błaszkiewicz 1999 ; Toh et al 1977 ; Gabbiani et al 1984 ; Brittingham et al 1997 ). Cytoplasmic β-actin seems to be overexpressed in many tumors, especially in actively moving cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Effect of overexpression of β- and γ-actin isoforms on actin cytoskeleton organization and migration of human colon cancer cells

Simiczyjew

Mazur

Popow-Woźniak

et al. 2014

Histochem Cell Biol

View full text Add to dashboard Cite

Actins are eukaryotic proteins, which are involved in diverse cellular functions including muscle contraction, cell motility, adhesion and maintenance of cell shape. Cytoplasmic actin isoforms β and γ are ubiquitously expressed and essential for cell functioning. However, their unique contributions are not very well understood. The aim of this study was to determine the effect of β- and γ-actin overexpression on the migration capacity and actin cytoskeleton organization of human colon adenocarcinoma BE cells. In cells overexpressing β- or γ-actin, distinct cytoskeletal actin rearrangements were observed under the laser scanning confocal microscope. Overexpressed actins localized at the submembranous region of the cell body, especially near to the leading edge and on the tips of pseudopodia. The cells transfected with plasmids containing cDNA for β- or γ-actin were characterized by increased migration and invasion capacities. However, the migration velocity was statistically significantly higher only in the case of γ-actin overexpressing cells. In conclusion, the increased level of β- or γ-actin leads to actin cytoskeletal remodeling followed by an increase in migration and invasion capacities of human colon BE cells. These data suggest that expression of both actin isoforms has an impact on cancer cell motility, with the subtle predominance of γ-actin, and may influence invasiveness of human colon cancer.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of overexpression of β- and γ-actin isoforms on actin cytoskeleton organization and migration of human colon cancer cells

Simiczyjew

Mazur

Popow-Woźniak

et al. 2014

Histochem Cell Biol

View full text Add to dashboard Cite

show abstract

“…In experimental animals, phalloidin and cytochalasin B treatments make bile canalicular F-actin either overpolymerized or depolymerized, respectively, to cause intracanalicular MV disappearance and intrahepatic jaundice (4,10,19,23). Abnormality of bile canalicular F-actin was frequently observed in patients and animals with obstructive jaundice, drug induced liver injury and other hepatic disorders (9,31). Recently, the contraction motility of BC was observed both in vitro and in vivo (33)(34)(35).…”

mentioning

confidence: 99%

Development of the Bile Canalicular System in Rat Liver: with Special Reference to Bile Canalicular Actin Filaments and Mg2+, Ca2+-ATPase Activity.

Lee

Araki

Takashima

1993

Acta Histochem. Cytochem

View full text Add to dashboard Cite

Developmental changes of the bile canaliculi (BC) in rat (fetal 13th-21st day, postnatal and adult) livers were cytochemically examined by fluorescence, light and electron microscopy. Bile canalicular actin filaments (F-actin), consisting of the circumferential pericanalicular F-actin and microvilli (MV) core F-actin, were observed by 7-nitrobenz-2-oxa-1,3-diazole phallacidin (NBD-ph) fluorescence microscopy and electron microscopy. Mg2+, Ca2+-ATPase activity, which is presumed to be involved in bile acid secretion across the bile canalicular membrane, was detected by a modified Wachstein and Meisel method at both light and electron microscopic levels. Bile canalicular F-actin sparsely appeared from the 13th day of gestation. Since then, the amount of bile canalicular F-actin gradually increased accompanying the bile canalicular formation by 2 weeks after the birth when the development was almost completed. Mg2+, Ca2+-ATPase activity appeared on the fetal 19th day in less than 10% of the BC. The number of Mg2+, Ca2+-ATPasepositive BC rapidly increased around the time of birth (fetal 21st day-postnatal 1st day). The rate of ATPase positive BC reached 40-70% during this period. On the 5th day after birth, most BC showed Mg2+, Ca2+-ATPase activity. These findings indicated that the expression of bile canalicular F-actin and ATPase can be regarded as a structural marker and a functional marker for bile secretion, respectively. Structural development of BC began earlier but at a rather slower rate than functional maturation.

show abstract

Comparative immunohistochemical localization of fibronectin and actin in human breast tumor cells in vivo and in vitro

Gibert

Noe͏̈l

Faucon

et al. 1982

Virchows Archiv B Cell Pathol

View full text Add to dashboard Cite

Increased expression of actin-like contractile protein in preneoplastic and neoplastic lesions in rat liver

Cited by 6 publications

References 14 publications

Effect of overexpression of β- and γ-actin isoforms on actin cytoskeleton organization and migration of human colon cancer cells

Effect of overexpression of β- and γ-actin isoforms on actin cytoskeleton organization and migration of human colon cancer cells

Development of the Bile Canalicular System in Rat Liver: with Special Reference to Bile Canalicular Actin Filaments and Mg2+, Ca2+-ATPase Activity.

Comparative immunohistochemical localization of fibronectin and actin in human breast tumor cells in vivo and in vitro

Contact Info

Product

Resources

About