SmbHLH53 is relevant to jasmonate signaling and plays dual roles in regulating the genes for enzymes in the pathway for salvianolic acid B biosynthesis in Salvia miltiorrhiza

Peng, Jingjing; Wu, Yucui; Wang, Shiqiang; Niu, Junfeng; Cao, Xiaoyan

doi:10.1016/j.gene.2020.144920

Cited by 18 publications

(18 citation statements)

References 46 publications

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“…Two-month-old S. miltiorrhiza plantlets were treated with 0.1 mM IAA, 0.1 mM GA 3 , 5 mM MeJA, or 0.1 mM ABA as previously described [ 53 ], which were then collected for RNA extraction following treatment for 10 min, 30 min, 1 h, 2 h, 3 h, and 9 h, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The 1146 bp promoter fragment of SmCYP98A14 was inserted into the Sma I and Mlu I (TaKaRa, Beijing, China) sites of the pHIS vector to generate the recombinant pHIS2- pCYP98A14 . The pHIS2- p4CL9 was obtained in an earlier study [ 53 ]. The recombinant vector pairs were co-transformed into Saccharomyces cerevisiae strain Y187 (Weidi Biotechnology, Shanghai, China) via a lithium acetate mediated method [ 58 ].…”

Section: Methodsmentioning

confidence: 99%

“…The promoter fragment of SmCYP98A14 was cloned into the Hin dIII and Bam HI (TaKaRa, Beijing, China) sites of pGreenII 0800-LUC vector as a report vector. The recombinant vector of pSm4CL9 -pGreenII 0800 LUC was synthesized as per our previous report [ 53 ]. The synthesized effector and reporter vectors were subsequently co-transferred into tobacco leaves via an Agrobacterium -mediated transformation.…”

Section: Methodsmentioning

confidence: 99%

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SmSPL6 Induces Phenolic Acid Biosynthesis and Affects Root Development in Salvia miltiorrhiza

Cao

Chen

Wang

et al. 2021

IJMS

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Salvia miltiorrhiza is a renowned model medicinal plant species for which 15 SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) family genes have been identified; however, the specific functions of SmSPLs have not been well characterized as of yet. For this study, the expression patterns of SmSPL6 were determined through its responses to treatments of exogenous hormones, including indole acetic acid (IAA), gibberellic acid (GA3), methyl jasmonic acid (MeJA), and abscisic acid (ABA). To characterize its functionality, we obtained SmSPL6-ovexpressed transgenic S. miltiorrhiza plants and found that overexpressed SmSPL6 promoted the accumulation of phenolic acids and repressed the biosynthesis of anthocyanin. Meanwhile, the root lengths of the SmSPL6-overexpressed lines were significantly longer than the control; however, both the fresh weights and lateral root numbers decreased. Further investigations indicated that SmSPL6 regulated the biosynthesis of phenolic acid by directly binding to the promoter regions of the enzyme genes Sm4CL9 and SmCYP98A14 and activated their expression. We concluded that SmSPL6 regulates not only the biosynthesis of phenolic acids, but also the development of roots in S. miltiorrhiza.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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SmSPL6 Induces Phenolic Acid Biosynthesis and Affects Root Development in Salvia miltiorrhiza

Cao

Chen

Wang

et al. 2021

IJMS

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“…The 1449-bp and 1652-bp promoter regions of SmHPPR1 and SmC4H1, respectively, were amplified and inserted into the pHIS2. The pHIS2-SmTAT1 and pHIS2-Sm4CL9 were constructed in our earlier study [29,50].…”

Section: Y1h Assaysmentioning

confidence: 99%

“…The promoter regions of SmHPPR1 and SmC4H1 were then inserted into the pGreenII 0800 empty vector. The recombinant vectors of pSmTAT1-pGreenII 0800 LUC and pSm4CL9-pGreenII 0800 LUC were constructed in our previous reports [29,50].…”

Section: Dual-luciferase Assaymentioning

confidence: 99%

R2R3-MYB Transcription Factor SmMYB52 Positively Regulates Biosynthesis of Salvianolic Acid B and Inhibits Root Growth in Salvia miltiorrhiza

Yang

Wang

Zou

et al. 2021

IJMS

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The dried root of Salvia miltiorrhiza is a renowned traditional Chinese medicine that was used for over 1000 years in China. Salvianolic acid B (SalB) is the main natural bioactive product of S. miltiorrhiza. Although many publications described the regulation mechanism of SalB biosynthesis, few reports simultaneously focused on S. miltiorrhiza root development. For this study, an R2R3-MYB transcription factor gene (SmMYB52) was overexpressed and silenced, respectively, in S. miltiorrhiza sterile seedlings. We found that SmMYB52 significantly inhibited root growth and indole-3-acetic acid (IAA) accumulation, whereas it activated phenolic acid biosynthesis and the jasmonate acid (JA) signaling pathway. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses revealed that SmMYB52 suppressed the transcription levels of key enzyme-encoding genes involved in the IAA biosynthetic pathway and activated key enzyme-encoding genes involved in the JA and phenolic acid biosynthesis pathways. In addition, yeast one-hybrid (Y1H) and dual-luciferase assay showed that SmMYB52 directly binds to and activates the promoters of several key enzyme genes for SalB biosynthesis, including SmTAT1, Sm4CL9, SmC4H1, and SmHPPR1, to promote the accumulation of SalB. This is the first report of a regulator that simultaneously affects root growth and the production of phenolic acids in S. miltiorrhiza.

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The Combined Effects of Rhizobacteria and Methyl Jasmonate on Rosmarinic Acid Production and Gene Expression Profile in Origanum Vulgare l. Under Salinity Conditions

Jafari

Arani

Esfahani

2022

J Plant Growth Regul

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SmbHLH53 is relevant to jasmonate signaling and plays dual roles in regulating the genes for enzymes in the pathway for salvianolic acid B biosynthesis in Salvia miltiorrhiza

Cited by 18 publications

References 46 publications

SmSPL6 Induces Phenolic Acid Biosynthesis and Affects Root Development in Salvia miltiorrhiza

SmSPL6 Induces Phenolic Acid Biosynthesis and Affects Root Development in Salvia miltiorrhiza

R2R3-MYB Transcription Factor SmMYB52 Positively Regulates Biosynthesis of Salvianolic Acid B and Inhibits Root Growth in Salvia miltiorrhiza

The Combined Effects of Rhizobacteria and Methyl Jasmonate on Rosmarinic Acid Production and Gene Expression Profile in Origanum Vulgare l. Under Salinity Conditions

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