Small-extracellular vesicles and their microRNA cargo from porcine follicular fluids: the potential association with oocyte quality

Gad, Ahmed; Murín, Matej; Bartková, Alexandra; Kinterová, Veronika; Marcollová, Kateřina; Laurinčík, Jozef; Procházka, Radek

doi:10.1186/s40104-022-00723-1

Cited by 8 publications

(3 citation statements)

References 68 publications

(77 reference statements)

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“…24,25 Small EVs (sEVs) contain miRNAs and are widely used as reliable biomarkers. 26 In a recent study, compared with healthy controls, colorectal cancer patients showed significantly increased miR-222-3p levels in plasma, which decreased effectively after surgical tumor removal. 27 Circulating sEV-derived miR-222-3p can serve as a promising noninvasive diagnostic biomarker for inflammatory breast cancer.…”

Section: Discussionmentioning

confidence: 98%

Dysregulated miR-222-3p in plasma exosomes of preeclampsia patients and its In vitro effect on HTR8/SVneo extravillous trophoblast cells by targeting STMN1

et al. 2022

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Objective To investigate the diagnostic efficiency of miR-222–3p in plasma exosomes (Exos) and plasma for preeclampsia (PE) and the effect of miR-222–3p targeting STMN1 in PE Methods MiR-222–3p levels in total plasma and plasma Exos were detected in PE patients and healthy controls. A bioinformatics database and dual-luciferase reporter assay were employed to verify the targeting relationship between miR-222–3p and STMN1. Trophoblast HTR-8/Svneo cells were transfected with miR-222–3p inhibitors with/without STMN1 shRNA, followed by MTT, wound healing and Transwell invasion assays. The mRNA and protein expressions were measured by qRT‒PCR and Western blotting, respectively. Results MiR-222–3p levels in total plasma and plasma Exos were higher in PE patients than in healthy controls, particularly in severe PE patients. In addition, miR-222–3p levels in total plasma and plasma Exos from PE patients were positively correlated with diastolic and systolic blood pressure. The area under the curve (AUC) of miR-222–3p in total plasma for PE diagnostic efficiency was 0.798, with a sensitivity of 76.67% and specificity of 71.93%, while the AUC of miR-222–3p in plasma Exos was 0.708 (sensitivity: 61.67%; specificity: 78.95%). In vitro, miR-222–3p targeted STMN1 in HTR-8/Svneo cells. Low miR-222–3p expression reversed the inhibitory effect of STMN1 shRNA on the proliferation, invasion and migration of HTR/SVneo cells. Conclusion PE patients had increased miR-222–3p expression in total plasma and plasma Exos, which both have high diagnostic efficiency for PE. MiR-222–3p can target STMN1 to promote the proliferation, invasion and migration of HTR-8/Svneo cells and is a potential therapeutic target of PE.

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Section: Discussionmentioning

confidence: 98%

Dysregulated miR-222-3p in plasma exosomes of preeclampsia patients and its In vitro effect on HTR8/SVneo extravillous trophoblast cells by targeting STMN1

et al. 2022

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“…FF-EVs primarily reflect oocyte quality through its content ( Rodrigues et al, 2019 ; Battaglia et al, 2020 ). For example, because the amount and type of miRNA and lipid carried by FF-EVs differ between high and low quality follicles ( da Silveira et al, 2021 ; Gad et al, 2022 ; Zhang et al, 2021 ), miRNA expression in FF-EVs differ between oocytes with and without successful fertilization ( Martinez et al, 2018 ). Thus, we were able to assess oocyte developmental potential and fertilization ability using FF-EVs.…”

Section: Extracellular Vesicles’ Clinical Utility In Assisted Reprodu...mentioning

confidence: 99%

Messenger roles of extracellular vesicles during fertilization of gametes, development and implantation: Recent advances

Fan

Wang

et al. 2023

Front. Cell Dev. Biol.

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Extracellular vesicles (EVs) have become a research hotspot in recent years because they act as messengers between cells in the physiological and pathological processes of the human body. It can be produced by the follicle, prostate, embryo, uterus, and oviduct in the reproductive field and exists in the extracellular environment as follicular fluid, semen, uterine cavity fluid, and oviduct fluid. Because extracellular vesicles are more stable at transmitting information, it allows all cells involved in the physiological processes of embryo formation, development, and implantation to communicate with one another. Extracellular vesicles carried miRNAs and proteins as mail, and when the messenger delivers the mail to the recipient cell, the recipient cell undergoes a series of changes. Current research begins with intercepting and decoding the information carried by extracellular vesicles. This information may help us gain a better understanding of the secrets of reproduction, as well as assist reproductive technology as an emerging marker and treatment.

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“…As a result, maternal RNAs that accumulate in the oocyte are required for chromatin reorganization and transcription factor translation (Eckersley-Maslin et al, 2018;Østrup et al, 2012). Despite attempts to estimate oocyte potential using mural granulosa, cumulus cells, extracellular vesicles from follicular fluid, and other follicular niche components, results are conflicting and unable to correlate precisely which molecules in the ooplasm are responsible for the oocyte's ability to develop to the blastocyst stage (Gad et al, 2022;Martínez-Moro et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Retrospective model utilizing biopsies, granulosa cells, and polar body to predict oocyte competence in bovine

Ambrizi

Nociti

et al. 2023

Preprint

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Developmental competence is obtained by a series of morphological and molecular changes during mammalian oocyte growth within the ovulatory follicle. This entails the accumulation of cytoplasmic transcripts that will be used throughout the early stages of development prior to embryonic genome activation, a process known as ooplasm maturation. Furthermore, during follicular growth, epigenetic maturation occurs, which is essential for appropriate embryo development. We believe that transcripts and DNA methylation differ between blastocyst oocytes and those that cleaved but were arrested on day three. We devised a retrospective technique to identify transcripts in oocyte, cumulus, and granulosa cells, as well as DNA methylation connected with oocyte competence, in this work. We dissected and harvested ovarian follicles to achieve this purpose. We extracted and flash frozen the granulosa cells after rupturing them. The oocytes were put in maturation media droplets, and the cumulus cells and polar body were removed and kept the following day. To prevent spermatozoon interference, we chemically activated the oocytes and tracked their development (until they reached the blastocyst stage). We went back to their biopsies, cumulus cells, and polar bodies and did RNA-seq (biopsies and cumulus) and single polar body WGBS (polar bodies) when we collected the results 7 days later, i.e. 1-) embryos that cleaved but stopped development (termed CL) or 2-) embryos that cleaved and progressed until the blastocyst stage (termed BL). Additionally, after transcriptome results from oocyte-biopsy and cumulus cells, the granulosa cells from their individual oocytes were sequenced as a noninvasive strategy. This study is a follow-up to our previous work, "Assessment of Total Oocyte Transcripts Representation in bovine Using Single Ooplasm Biopsy with High Reliability." Following sequencing, we discovered that the two groups, BL x CL, were transcriptionally different in granulosa and biopsy samples, although cumulus cells were a poor predictor of oocyte competence. By analyzing the differentially expressed genes, we discovered multiple genes and pathways related to oocyte competency, demonstrating the efficacy of our method. Despite no change in morphology, these alterations in pathways and genes show that the oocyte CL group was transcriptionally and epigenetically delayed, with ferroptosis and necroptosis processes activated. The oocyte BL group demonstrated numerous molecular signaling, oocyte meiosis, GnRH signaling, G-protein cascade, and RNA stability pathways. In network analysis, we discovered GNAS, an imprinted gene and one of the most important essential genes. The transcripts from granulosa cells confirm the oocyte results. Nonetheless, transcriptional variations in granulosa cells were far greater than those in oocytes (97% x 34% variance), implying a completely distinct transcriptome in the follicular niche. In terms of the WGBS, we discovered differentially methylated areas linked with oocyte competency, as well as transcriptome results confirming the structure's ability to predict outcome. These findings might be beneficial in clinical settings for those undergoing infertility therapy. In the oocyte, we discovered a complex transcriptional and epigenetic regulation network. Furthermore, mature cumulus transcription produced information that differed from the true content of the MII oocyte and granulosa cells before maturity. Our findings underscore the significance of maternal transcripts and epigenetic maturation in early parthenogenesis, as well as the use of granulosa cells as early indicators of competence.

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Small-extracellular vesicles and their microRNA cargo from porcine follicular fluids: the potential association with oocyte quality

Cited by 8 publications

References 68 publications

Dysregulated miR-222-3p in plasma exosomes of preeclampsia patients and its In vitro effect on HTR8/SVneo extravillous trophoblast cells by targeting STMN1

Dysregulated miR-222-3p in plasma exosomes of preeclampsia patients and its In vitro effect on HTR8/SVneo extravillous trophoblast cells by targeting STMN1

Messenger roles of extracellular vesicles during fertilization of gametes, development and implantation: Recent advances

Retrospective model utilizing biopsies, granulosa cells, and polar body to predict oocyte competence in bovine

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